Effects of manganese on maturation of porcine oocytes &lt;i&gt;in vitro&lt;/i&gt; and their subsequent embryo development after parthenogenetic activation and somatic cell nuclear transfer

Qasim, Muhammad; Jin, Jun-Xue; Lee, Sanghoon; Taweechaipaisankul, Anukul; Setyawan, Erif Maha Nugraha; Lee, Byeong Chun

doi:10.1262/jrd.2019-001

Cited by 6 publications

(3 citation statements)

References 53 publications

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“…A previous study suggested that SOD1 deficiency increased superoxide generation, thereby exhibiting abnormal embryo development in murine oocytes [ 36 , 37 ]. Therefore, our results were consistent with most experiments on antioxidant supplementation, which indicated that antioxidants provided advantageous microenvironment to improve oocyte maturation by protecting oocytes against ROS in pigs [ 19 , 33 , 38 , 39 ]. Indeed, supplementation of melatonin during IVM could improve oocyte maturation and their subsequent embryo development by reducing oxidative stress and DNA damage in various species [ 40 , 41 , 42 , 43 ].…”

Section: Discussionsupporting

confidence: 90%

Ramelteon Reduces Oxidative Stress by Maintenance of Lipid Homeostasis in Porcine Oocytes

et al. 2022

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This study aimed to determine the underlying mechanism of ramelteon on the competence of oocyte and subsequent embryo development in pigs during in vitro maturation (IVM). Our results showed that the cumulus expansion index was significantly lower in the control group compared to the ramelteon groups (p < 0.05). Moreover, supplementation of 10−11 and 10−9 M ramelteon significantly increased the cumulus expansion and development-related genes expression, and reduced apoptosis in cumulus cells (p < 0.05). In oocytes, the nuclear maturation rate was significantly improved in 10−11, 10−9, and 10−7 M ramelteon groups compared to the control (p < 0.05). Additionally, the level of intracellular GSH was significantly increased and ROS was significantly decreased in ramelteon-supplemented groups, and the gene expression of oocyte development and apoptosis were significantly up- and down-regulated by 10−11 and 10−9 M ramelteon (p < 0.05), respectively. The immunofluorescence results showed that the protein levels of GDF9, BMP15, SOD1, CDK1, and PGC1α were significantly increased by 10−11 M ramelteon compared to the control (p < 0.05). Although there was no significant difference in cleavage rate, the blastocyst formation rate, total cell numbers, and hatching/-ed rate were significantly improved in 10−11 M ramelteon group compared to the control (p < 0.05). Furthermore, embryo development, hatching, and mitochondrial biogenesis-related genes were dramatically up-regulated by 10−11 M ramelteon (p < 0.05). In addition, the activities of lipogenesis and lipolysis in oocytes were dramatically increased by 10−11 M ramelteon compared to the control (p < 0.05). In conclusion, supplementation of 10−11 M ramelteon during IVM improved the oocyte maturation and subsequent embryo development by reducing oxidative stress and maintenance of lipid homeostasis.

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Section: Discussionsupporting

confidence: 90%

Ramelteon Reduces Oxidative Stress by Maintenance of Lipid Homeostasis in Porcine Oocytes

et al. 2022

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“…The effect of enrichment on the follicular environment can be two-sided. Mn was found to be beneficial to the development of oocytes, ,− and the Ni 2+ binding protein pNiXa can induce oocyte maturation in Xenopus . These enrichment phenomena suggest the special demands of the metal balance during oocyte development.…”

Section: Discussionmentioning

confidence: 99%

Using Machine Learning to Construct the Blood–Follicle Distribution Models of Various Trace Elements and Explore the Transport-Related Pathways with Multiomics Data

Zhang,

Lin,

Gao

et al. 2024

Environ. Sci. Technol.

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Permeabilities of various trace elements (TEs) through the blood−follicle barrier (BFB) play an important role in oocyte development. However, it has not been comprehensively described as well as its involved biological pathways. Our study aimed to construct a blood−follicle distribution model of the concerned TEs and explore their related biological pathways. We finally included a total of 168 women from a cohort of in vitro fertilization-embryo transfer conducted in two reproductive centers in Beijing City and Shandong Province, China. The concentrations of 35 TEs in both serum and follicular fluid (FF) samples from the 168 women were measured, as well as the multiomics features of the metabolome, lipidome, and proteome in both plasma and FF samples. Multiomics features associated with the transfer efficiencies of TEs through the BFB were selected by using an elastic net model and further utilized for pathway analysis. Various machine learning (ML) models were built to predict the concentrations of TEs in FF. Overall, there are 21 TEs that exhibited three types of consistent BFB distribution characteristics between Beijing and Shandong centers. Among them, the concentrations of arsenic, manganese, nickel, tin, and bismuth in FF were higher than those in the serum with transfer efficiencies of 1.19−4.38, while a reverse trend was observed for the 15 TEs with transfer efficiencies of 0.076−0.905, e.g., mercury, germanium, selenium, antimony, and titanium. Lastly, cadmium was evenly distributed in the two compartments with transfer efficiencies of 0.998−1.056. Multiomics analysis showed that the enrichment of TEs was associated with the synthesis and action of steroid hormones and the glucose metabolism. Random forest model can provide the most accurate predictions of the concentrations of TEs in FF among the concerned ML models. In conclusion, the selective permeability through the BFB for various TEs may be significantly regulated by the steroid hormones and the glucose metabolism. Also, the concentrations of some TEs in FF can be well predicted by their serum levels with a random forest model.

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“…However, there was no substantial difference in the cleavage rate and total cell numbers in blastocysts compared to the untreated group. Manganese improved the developmental competence of cloned porcine embryos by increasing GSH and decreasing ROS levels [232].…”

Section: Alternative Methods For Cloning Efficiency Improvementmentioning

confidence: 98%

Strategies to Improve the Efficiency of Somatic Cell Nuclear Transfer

Srirattana

Kaneda

Parnpai

2022

IJMS

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Mammalian oocytes can reprogram differentiated somatic cells into a totipotent state through somatic cell nuclear transfer (SCNT), which is known as cloning. Although many mammalian species have been successfully cloned, the majority of cloned embryos failed to develop to term, resulting in the overall cloning efficiency being still low. There are many factors contributing to the cloning success. Aberrant epigenetic reprogramming is a major cause for the developmental failure of cloned embryos and abnormalities in the cloned offspring. Numerous research groups attempted multiple strategies to technically improve each step of the SCNT procedure and rescue abnormal epigenetic reprogramming by modulating DNA methylation and histone modifications, overexpression or repression of embryonic-related genes, etc. Here, we review the recent approaches for technical SCNT improvement and ameliorating epigenetic modifications in donor cells, oocytes, and cloned embryos in order to enhance cloning efficiency.

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Effects of manganese on maturation of porcine oocytes <i>in vitro</i> and their subsequent embryo development after parthenogenetic activation and somatic cell nuclear transfer

Cited by 6 publications

References 53 publications

Ramelteon Reduces Oxidative Stress by Maintenance of Lipid Homeostasis in Porcine Oocytes

Ramelteon Reduces Oxidative Stress by Maintenance of Lipid Homeostasis in Porcine Oocytes

Using Machine Learning to Construct the Blood–Follicle Distribution Models of Various Trace Elements and Explore the Transport-Related Pathways with Multiomics Data

Strategies to Improve the Efficiency of Somatic Cell Nuclear Transfer

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