Effects of Maternal Marginal Iodine Deficiency on Dendritic Morphology in the Hippocampal CA1 Pyramidal Neurons in Rat Offspring

Min, Hui; Wang, Yi; Dong, Jing; Wang, Yuan; Yu, Yong; Shan, Zhongyan; Xi, Qi; Wang, Teng; Chen, Jie

doi:10.1007/s12017-016-8391-0

Cited by 7 publications

(4 citation statements)

References 57 publications

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“…And each litter was culled to about eight pups on PN4 (same number of males and females in each group, if possible). These marginal, mild, and severe ID animal models were successfully obtained in our previous researches (Dong et al, 2016; Min et al, 2016b, 2017). …”

Section: Methodsmentioning

confidence: 88%

Maternal Different Degrees of Iodine Deficiency during Pregnant and Lactation Impair the Development of Cerebellar Pinceau in Offspring

et al. 2017

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Aims: Iodine is critical for synthesis of thyroid hormones (TH). And iodine deficiency (ID) is one of the most significant reasons of intellectual disability and motor memory impairment, although the potential mechanisms are still under investigation. Presently, mild ID and marginal ID are largely ignored problems for women of child bearing age. Mild ID is a subtle form of TH deficiency, which shows low levels of free thyroxine (FT4) and relatively normal free triiodothyronine (FT3) or thyroid stimulation hormone (TSH). And marginal ID is a milder form of ID with decreased total T4 (TT4) but relatively normal FT3, FT4, and TSH. Therefore, we investigated the effects of maternal different degrees of ID on the development of pinceau in cerebellar purkinje cells (PCs) and studied the expression of pinceau related protein, which is crucial for the development and maturation of pinceau.Methods and Results: Three developmental iodine deficient rat models were created by feeding dam rats with an iodine-deficient diet and deionized water supplemented with potassiumiodide. Our study showed that different degrees of ID inhibited cerebellar pinceau synapse development and maturation on postnatal day (PN) 14 and PN21. What's more, mild and severe ID reduced the expression of AnkG, β4-spectrin, neurofascin186 and NrCAM on PN7, PN14, and PN21. However, marginal ID rarely altered expression of these proteins in the offspring.Conclusion: These results suggested that maternal mild and severe ID impaired the development and maturation of cerebellar pinceau, which may be attributed to the decrease of AnkG, β4-spectrin, neurofascin 186, and NrCAM. And the alteration of development and maturation in cerebellar pinceau in the offspring were also observed following maternal marginal ID, which is slighter than that of mild ID.

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Section: Methodsmentioning

confidence: 88%

Maternal Different Degrees of Iodine Deficiency during Pregnant and Lactation Impair the Development of Cerebellar Pinceau in Offspring

et al. 2017

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“…However, for higher risk groups i.e . pregnant women and unborn children, a negative impact may be expected such as hypothyroidism and related CNS impairment, decrease in TSH and T4 and change in behaviour were noted 8,25–28 . The molecular mechanisms that drive these AO are currently unknown and need further investigation to propose adapted/optimised KI administration.…”

Section: Discussionmentioning

confidence: 99%

Effects of repetitive Iodine Thyroid Blocking on the Development of the Foetal Brain and Thyroid in rats: a Systems Biology approach

Cohen

Benadjaoud

Lestaevel

et al. 2019

Preprint

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A single dose of potassium iodide (KI) against repeated exposure to radioactive iodine, such as the one of the Fukushima accident, might not be effective enough to protect the thyroid.Our group showed that repetitive dose of KI for eight days offers efficient protection without toxic effects in adult rats. However, the effect of repetitive KI on the developing foetus still unknown especially on brain development, but a correlation between the impaired maternal thyroid status and a decrease in intelligence quotient has been observed. In this study, gene expression analysis of the progeny of repetitive KI-administered pregnant rats performed by our group showed distinct gene expression profile from two different organs: thyroid and cortex. To understand how these differentially expressed genes are implicated in the observed behaviour change, a systems biology approach was used to construct networks using three different techniques; Bayesian statistics using ShrinkNet, sPLS-DA on the DIABLO platform using mixOmics and manual construction of a Process Descriptive network. For each organ, we were able to construct gene expression network, to select genes that are most contributing to either control or KI-treated groups, respectively, and to construct the PD network from differentially expressed (DE) gene enriched with data from publications. Furthermore, we were able to connect DE genes from both organs into one network with genes from both organ participating in the same cellular processes that affect mitophagy and neuronal outgrowth.This work may help to evaluate the doctrine for using KI in case of repetitive or prolonged exposure to radioactive particles upon nuclear accidents.

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“…However, for groups at higher risk i.e. pregnant women and unborn children, a negative impact may be expected such as hypothyroidism and related CNS impairment, decrease in TSH and T4, as well as a change in behaviour [8][9][10][11][12] . The molecular mechanisms that drive these AO are currently unknown and need further investigation to propose adapted/optimised KI prophylaxis.…”

Section: Discussionmentioning

confidence: 99%

Effects of repetitive Iodine thyroid blocking on the foetal brain and thyroid in rats: a systems biology approach

Cohen

Benadjaoud

Lestaevel

et al. 2020

Sci Rep

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A single administration of an iodine thyroid blocking agent is usually sufficient to protect thyroid from radioactive iodine and prevent thyroid cancer. Repeated administration of stable iodine (rKI) may be necessary during prolonged or repeated exposure to radioactive iodine. We previously showed that rKI for eight days offers protection without toxic effects in adult rats. However, the effect of rKI administration in the developing foetus is unknown, especially on brain development, although a correlation between impaired maternal thyroid status and a decrease in intelligence quotient of the progeny has been observed. This study revealed distinct gene expression profiles between the progeny of rats receiving either rKI or saline during pregnancy. To understand the implication of these differentially expressed (DE) genes, a systems biology approach was used to construct networks for each organ using three different techniques: Bayesian statistics, sPLS-DA and manual construction of a Process Descriptive (PD) network. The PD network showed DE genes from both organs participating in the same cellular processes that affect mitophagy and neuronal outgrowth. This work may help to evaluate the doctrine for using rKI in case of repetitive or prolonged exposure to radioactive particles upon nuclear accidents. During a nuclear emergency, large amounts of radioactive particles may be released and may contaminate the environment and populations. Isotopes present in the atmospheric discharges include radioactive iodine-131. To prevent health consequences, one of the main protective methods is the Iodine Thyroid Blocking (ITB) agent which aims to saturate the thyroid gland with non-radioactive iodine and thus avoiding the fixation of radioactive iodine (Wolff-Chaikoff effect) 1. However, the recent 2011 Fukushima Daiichi disaster suggested that the World Health Organization's "ITB guidelines" 2 that so far supported a single intake of 130 mg potassium iodide (KI) tablet for individuals older than 12 years, cannot adequately protect populations in case of prolonged (beyond 24 h) or repeated exposure. In such situations, a second intake of stable iodine is possible, although the "ITB guidelines" so far give no indication on how to implement such repeated administration of KI 2,3. Therefore, more knowledge about repetitive stable iodine administration is essential to prevent the side effects of repeated ITB on specific sensitive sub-populations such as pregnant women, foetusses, and elderly 3. Recently, preclinical data, obtained by our group, have showed that repeated ITB procedure with a daily administration of 1 mg/kg/day 4,5 for eight days of KI does not induce adverse outcome (AO) in adult rats 6,7. However, for higher risk groups such as pregnant women and unborn children, a negative impact may be expected, such as hypothyroidism and related CNS impairment, decrease in TSH and T 4-thyroid hormone, and change in behaviour 8-12. Repeated ITB administration for eight consecutive days in gestational rats, as a model for pregn...

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Effects of Maternal Marginal Iodine Deficiency on Dendritic Morphology in the Hippocampal CA1 Pyramidal Neurons in Rat Offspring

Cited by 7 publications

References 57 publications

Maternal Different Degrees of Iodine Deficiency during Pregnant and Lactation Impair the Development of Cerebellar Pinceau in Offspring

Maternal Different Degrees of Iodine Deficiency during Pregnant and Lactation Impair the Development of Cerebellar Pinceau in Offspring

Effects of repetitive Iodine Thyroid Blocking on the Development of the Foetal Brain and Thyroid in rats: a Systems Biology approach

Effects of repetitive Iodine thyroid blocking on the foetal brain and thyroid in rats: a systems biology approach

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