Effects of maternal uninephrectomy on the development of fetal rat kidney: apoptosis and the expression of oncogenes

Okada, Toshiya; Mitsuoka, Koji; Mino, Masaki; Mukamoto, Masafumi; Nakamura, Jun; Morioka, Hiroshi; Morikawa, Yoshio

doi:10.1111/j.1741-4520.2006.00101.x

Cited by 3 publications

(5 citation statements)

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“…In this experiment, we proposed an accelerated renal development in the melamine‐affected neonatal kidney. Accelerated renal development is shown to be associated with maternal renal dysfunction caused by bilateral ureteral ligation and maternal uninephrectomy . Maternal uninephrectormy‐related accelerated glomerular development and the accelerated glomerular function will lead to stimulation on the development of the proximal tubules, dilation of the tubular lumen and differentiation of the tubular epithelium of the loops of Henle .…”

Section: Discussionmentioning

confidence: 99%

“…In this experiment, we proposed an accelerated renal development in the melamine-affected neonatal kidney. Accelerated renal development is shown to be associated with maternal renal dysfunction caused by bilateral ureteral ligation and maternal uninephrectomy 46,47. Maternal uninephrectormy-relatedFIG URE 1 Histological change of maternal melamine nephrotoxicity.…”

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confidence: 99%

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Effects of low‐dose melamine exposure during pregnancy on maternal and fetal kidneys in rats

Chu

Fung

Wang

2017

Environmental Toxicology

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Despite the previous reports on melamine contamination in high concentrations some years ago, there were not many studies on low-level exposure in daily life, particularly in pregnancy. We investigated the effect of low-dose melamine on the kidneys of the pregnant rats and their developing embryos/fetuses during various gestational stages namely implantation, gastrulation, organogenesis, maturation and whole pregnancy. Our results showed that the repeated low level of melamine (12.5, 25, and 50 mg/kg bw/d) during pregnancy did not cause obstruction of renal tubules although more precipitating crystals were found in the early gestational periods. Simple hyperplasia in the maternal tubules and pelvic epithelium were more prominent after exposed to melamine during the whole gestational period. Neonatal kidneys significantly suffered more from congestion in glomeruli and interstitium, dilated tubules and interstitial edema after melamine administration to the mother in the late and the whole gestational periods. A trend of advance of glomerular development in fetuses was also observed. We conclude that in utero exposure of low-level melamine could post a risk on the kidneys of the pregnant mother as well as the developing fetuses, which may further increase the possibility of other health problems later in life.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Effects of low‐dose melamine exposure during pregnancy on maternal and fetal kidneys in rats

Chu

Fung

Wang

2017

Environmental Toxicology

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“…Recently, we have observed the increment of apoptosis associated with decreased expression of bcl-2 in the kidney of fetuses from uninephrectomized mothers [18]. Therefore, the present study suggests that EGF may play an important role in reduction of TUNEL positive cells in the Lp rats.…”

Section: Discussionmentioning

confidence: 66%

Effects of Low Protein Intake on the Development of the Remaining Kidney in Subtotally Nephrectomized Immature Rats: Apoptosis and Epidermal Growth Factor

Mino

Nakamura²,

Nakamuta

et al. 2007

The Journal of Veterinary Medical Science

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ABSTRACT. Effects of low protein intake on the development of the remaining kidney in subtotally (5/6) nephrectomized immature rats were examined. Three week-old weaning rats were kept on a diet containing either 12% (Lp rats) or 18% (Np rats) protein for 4 or 8 weeks after subtotal nephrectomy. Blood urea nitrogen (BUN) concentration was determined at 2, 4, 6, and 8 weeks after 5/6 nephrectomy. At 4 or 8 weeks after the operation, glomerular sclerosis and tubulointerstitial damage were assessed by a standard semiquantitative analysis and were expressed as the glomerular sclerosis index (GSI) and interstitial fibrosis score (IFS), respectively. The localization of DNA fragmented cells in the kidney was examined by the terminal deoxynucleotidyl transferase (TdT) -mediated d-UTPbiotin nick end labeling (TUNEL) method and the localization of the epidermal growth factor (EGF) by immunohistochemical methods. BUN concentration was significantly lower in the Lp rats compared with that in the Np rats. Both 4 and 8 weeks after subtotal nephrectomy, GSI and incidence of TUNEL positive cells in the distal tubules were significantly lower in the Lp rats than in the Np rats. Four weeks after the operation, IFS was significantly lower in the Lp rats than in the Np rats. Four and 8 weeks after the operation, EGF positive cells in the distal tubules were more observed in the Lp rats than in the Np rats. These findings reveal that protein restriction is effective in preventing renal tubular scarring in immature rats and that EGF is involved in the process of this prevention. KEY WORDS: EGF, immature rat, subtotal nephrectomy, TUNEL.J. Vet. Med. Sci. 69(3): 247-252, 2007 Subtotal (5/6) nephrectomy has been performed on laboratory animals to achieve a model for chronic renal failure [13,25]. The remaining kidney of 5/6 nephrectomized animals was utilized as a model for renal fibrosis [9,15,24] and a model for glomerular sclerosis [2,5]. In the remaining kidney glomerular sclerosis is observed following increased proliferative activity in glomeruli [2] and renal fibrosis is accompanied with apoptotic cells [12]. In this model, restriction of protein intake improves renal failure [6]. On the other hand, the epidermal growth factor (EGF) plays an important role in proliferative activity [16] and apoptosis [17] of the developing kidney in perinatal rats. These findings lead us to believe that both subtotal nephrectomy and restriction of protein intake greatly influence the developing kidney. Friedman and Pityer [3] have reported that low protein intake improves survival over normal protein intake in 3/4 nephrectomized immature rats. However, there is very little information available on the development of the remaining kidney in 5/6 nephrectomized immature rats. The present study was designed to clarify the effect of low protein intake on the development of the remaining kidney of subtotally nephrectomized immature rats with special reference to apoptosis and EGF. MATERIALS AND METHODS Animals and tissue processing:Three week-old m...

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“…Furthermore, we previously reported that maternal uninephrectomy induces lowered proliferative activity in mature glomerulus and enhanced positive reactions to both EGF and EGFR antibodies in proximal tubular cells in the fetal kidney (Okada et al, 2000). Recently, we have revealed that an increase in apoptosis in the collecting ducts of fetal kidney is induced by maternal uninephrectomy and that the increase is related to the decreased expression of bcl-2, an apoptotic suppressor gene (Okada et al, 2006).…”

Section: Effects Of Maternal Uninephrectomy On the Development Of Fetmentioning

confidence: 99%

“…Maternal bilateral ligation of the uterine artery, protein restriction, smoking, nephrotoxic medication as well as salt loading cause intrauterine growth retardation (IUGR) to fetuses (Bentz & Amann, 2010), inducing fetal programing of renal function. Previously, we investigated the development of the fetal kidney during maternal renal dysfunction induced by bilateral ureteral ligation (Okada et al, 1997) and uninephrectomy (Okada et al, 2000(Okada et al, , 2006, and we found that the development of the fetal kidney is accelerated by the operations. Furthermore, we found that maternal bilateral ureteral ligation (Okada & Morikawa, 1988) and uninephrectomy (Okada et al, 1998) decrease fetal body weight.…”

Section: Introductionmentioning

confidence: 99%

Effects of Maternal Renal Dysfunction on Fetal Development

Okada¹,

Kitano-Amahori²,

Mino³

et al. 2012

Renal Failure - The Facts

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Effects of maternal uninephrectomy on the development of fetal rat kidney: apoptosis and the expression of oncogenes

Cited by 3 publications

References 18 publications

Effects of low‐dose melamine exposure during pregnancy on maternal and fetal kidneys in rats

Effects of low‐dose melamine exposure during pregnancy on maternal and fetal kidneys in rats

Effects of Low Protein Intake on the Development of the Remaining Kidney in Subtotally Nephrectomized Immature Rats: Apoptosis and Epidermal Growth Factor

Effects of Maternal Renal Dysfunction on Fetal Development

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