Effects of mechanical and chemical methods on denture biofilm accumulation

Abstract

The aim of this study was to quantify biofilm on the internal surface of upper complete dentures following six possible cleansing methods. Thirty-six edentulous subjects were submitted to a time-series trial and dentures were cleansed according to six methods: (i) rinsing with water; (ii) soaking in an alkaline peroxide solution (Bonyplus); (iii) brushing with dentifrice (Dentu-Creme) and soft Johnson and Johnson's toothbrush; (iv) combination between soaking and brushing according to methods 2 and 3; (v) brushing with dentifrice (Dentu-Creme) and soft Oral B toothbrush; (vi) combination between soaking and brushing according to methods 2 and 5. Each method was randomly used throughout 21 days. Denture biofilm was disclosed by 1% neutral red solution and quantified by means of digital photos taken from the internal surface. The six methods presented significant differences in percentage of biofilm coverage (repeated measures anova, P < 0.0001). Method 1 showed the highest values, 2 was intermediate and other results were the lowest. The most efficacious approach was 6. Biofilm tended to accumulate predominantly over specific zones of the denture base, but this pattern did not change regardless of the method employed. It can be concluded that brushing alone was more effective than the chemical method employed. The best results were obtained by a combination of methods.

Color Stability, Surface Roughness and Flexural Strength of an Acrylic Resin Submitted to Simulated Overnight Immersion in Denture Cleansers

“…Several agents are indicated for denture biofilm removal, classified into mechanical and chemical agents (1). Cleaning by chemical method consists in immersing the denture in solutions with solvent, detergent, antibacterial and antifungal actions, and such solutions can be employed alone or in association with brushing or ultrasonic devices (2,3). There are two main classes of immersion denture cleansers: NaOCl and alkaline peroxides (4).…”

Denture Essential Kit: An Introduction

“…6 Various denture hygiene procedures are mentioned in the literature and these can be divided into mechanical and chemical methods. [7][8] A study conducted by Abhishek Apratim et al concluded that most common method of cleaning was mechanical either with water or with soap. 9 Frequency of cleaning with cleansing solutions was very low.…”

Effect of repeated cycles of chemical disinfection in microhardness of acrylic resins of complete denture base

“…However, many elderly subjects are affected by diseases that limit their manual ability [3][4] , thus requiring complementation of the mechanical cleaning with chemical substances in order to avoid the accumulation of dental microbial plaque on the oral mucosa membrane and on the surfaces of dentures, what may lead to the development of several diseases, such as candidiasis [5][6] .…”

“…An effective alternative method that has been currently used is microwave irradiation to fight several diseases and eliminate colonization by Candida albicans 4,[8][9][10][11][12] .…”