Effects of mechanical stimulation on the proliferation of bone marrow-derived human mesenchymal stem cells

Choi, Kyung-Min; Seo, Young-Kwon; Yoon, Hee-Hoon; Song, Kye‐Yong; Kwon, Soon-Yong; Lee, Hwa-Sung; Park, Jung-Keug

doi:10.1007/bf02931075

Cited by 23 publications

(20 citation statements)

References 44 publications

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“…Presently, little is known about the effects of tensile strain on the proliferation or apoptosis in MSCs. Several works have reported that an appropriate level of mechanical stimuli can promote or inhibit cell proliferation [17]. In this experiment, we demonstrated that higher cyclic tensile stress (43.5) for lasting 30 min could promote the proliferation of MSCs proliferation significantly and it seemed that the tensile strains possibly lead to an increase in collagen synthesis in MSCs in early stage.…”

Section: Mscs Viability and Proliferation Under Different Tensile Stressmentioning

confidence: 80%

“…The mechanical force was observed to play an important role in directing stem cell fate toward osteogenic, chondrogenic and myogenic lineages in MSCs [6]. Among the various mechanical forces, compressive loading [11,12,13], shear stress [14,15] and tensile stress [16,17] are commonly used as the bio-mimetic loadings that are related to physiological environment such as blood vessel and bone tissue. However, few studies are devoted to the investigation of tensile strain in guiding MSCs in proliferation and differentiation.…”

Section: Introductionmentioning

confidence: 99%

“…However, few studies are devoted to the investigation of tensile strain in guiding MSCs in proliferation and differentiation. Although a few methods were proposed to study the influence of mechanical stretch [16,17], they are still limited by the requirement of large instrumental set-up with less flexibility. Especially, these approaches are not suitable for performing parallel assays under multiple experimental conditions in advanced applications.…”

Section: Introductionmentioning

confidence: 99%

“…For a long time, biochemical factors such as growth factors, cytokines and other regulatory molecules have been well documented [7,8]. Despite such progresses in vivo and in vitro evidences, the extrinsic biophysical signals conducive to guide MSCs toward specific cell lineages have been investigated as well, including mechanical force [11][12][13][14][15][16][17], nanostructures [9,10], physical matrix characteristics, etc. The mechanical force was observed to play an important role in directing stem cell fate toward osteogenic, chondrogenic and myogenic lineages in MSCs [6].…”

Section: Introductionmentioning

confidence: 99%

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A simple elastic membrane‐based microfluidic chip for the proliferation and differentiation of mesenchymal stem cells under tensile stress

et al. 2011

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This work presents a simple membrane-based microfluidic chip for the investigation of proliferation and differentiation of mesenchymal stem cells (MSCs) under mechanical stimuli. The cyclic tensile stress was generated by the deformation of elastic PDMS membrane sandwiched between the two layer microfluidic chip via actuated negative pressure, and the cultured MSCs on membrane were subjected to different orders of tensile stress. The results suggest that mechanical stimuli are attributed to the different phenomena of MSCs in cell proliferation and differentiation. The higher tensile stress (>3.5) promoted obvious proliferation, osteogenesis and reduced adipogenesis in MSCs, indicating the possible regulative role of tensile stress in modifying the osteogenesis/adipogenesis balance in the development of tissue organ.

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Section: Mscs Viability and Proliferation Under Different Tensile Stressmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A simple elastic membrane‐based microfluidic chip for the proliferation and differentiation of mesenchymal stem cells under tensile stress

et al. 2011

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“…Tissue engineering is gaining popularity in Korea [18][19][20], as in the rest of the world [21]. The key to tissue engineering would be the utilization of knowledge gained on animal cell culture in addition to stem cell technology and oxygen transfer into dense tissues.…”

Section: Recent Trends In Animal Cell Culture and Tissue Engineering mentioning

confidence: 99%

Two-stage depth filter perfusion culture for recombinant antibody production by recombinant Chinese hamster ovary cell

Lee

Kim

et al. 2008

Biotechnol Bioproc E

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A rCHO cell line of DUKX origin 26*-320, producing recombinant antibody against the human platelet, was cultivated in a two-stage depth filter perfusion system (DFPS) for 20 days in order to attain high recombinant antibody concentration. The productivity of the first stage DFPS bioreactor reached 53 times that of the batch culture in a controlled stirred tank reactor and was showed 12.1 mg/L antibody concentration at a perfusion rate of 6.0 d −1 . Glucose concentration in the first DFPS was maintained at 1.5 g/L to avoid cell damage in the perfusion culture. A second stage DFPS system was attached to the first DFPS, which resulted in a low glucose concentration of 0.02 g/L and a high antibody concentration of 23.9 mg/L. The two-stage depth filter perfusion culture yielded 60% higher product concentration than the batch and 49-fold higher productivity of 69.3 mg/L/d in comparison with that (1.4 mg/L/d) in a batch system. Furthermore, antibody concentration of the second stage was 97% higher than that of the first stage, and the antibody productivities were comparable to that of the first stage. This two-stage DFPS system also showed potential for higher titer production of recombinant antibody and high volumetric productivity for long-term culture of bio-pharmaceutical substances. © KSBB hÉóïçêÇëW=`el=ÅÉääëI=éÉêÑìëáçå=ÅìäíìêÉI=êÉÅçãÄáå~åí=~åíáÄçÇóI=íïçJëí~ÖÉ=ÇÉéíÜ=ÑáäíÉê=éÉêÑìëáçå=ëóëíÉã= = = = =

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Dynamic Stimulations with Bioengineered Extracellular Matrix‐Mimicking Hydrogels for Mechano Cell Reprogramming and Therapy

Shou

Teo

et al. 2023

Advanced Science

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Cells interact with their surrounding environment through a combination of static and dynamic mechanical signals that vary over stimulus types, intensity, space, and time. Compared to static mechanical signals such as stiffness, porosity, and topography, the current understanding on the effects of dynamic mechanical stimulations on cells remains limited, attributing to a lack of access to devices, the complexity of experimental set-up, and data interpretation. Yet, in the pursuit of emerging translational applications (e.g., cell manufacturing for clinical treatment), it is crucial to understand how cells respond to a variety of dynamic forces that are omnipresent in vivo so that they can be exploited to enhance manufacturing and therapeutic outcomes. With a rising appreciation of the extracellular matrix (ECM) as a key regulator of biofunctions, researchers have bioengineered a suite of ECM-mimicking hydrogels, which can be fine-tuned with spatiotemporal mechanical cues to model complex static and dynamic mechanical profiles. This review first discusses how mechanical stimuli may impact different cellular components and the various mechanobiology pathways involved. Then, how hydrogels can be designed to incorporate static and dynamic mechanical parameters to influence cell behaviors are described. The Scopus database is also used to analyze the relative strength in evidence, ranging from strong to weak, based on number of published literatures, associated citations, and treatment significance. Additionally, the impacts of static and dynamic mechanical stimulations on clinically relevant cell types including mesenchymal stem cells, fibroblasts, and immune cells, are evaluated. The aim is to draw attention to the paucity of studies on the effects of dynamic mechanical stimuli on cells, as well as to highlight the potential of using a cocktail of various types and intensities of mechanical stimulations to influence cell fates (similar to the concept of biochemical cocktail to direct cell fate). It is envisioned that this progress report will inspire more exciting translational development of mechanoresponsive hydrogels for biomedical applications.

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Effects of mechanical stimulation on the proliferation of bone marrow-derived human mesenchymal stem cells

Cited by 23 publications

References 44 publications

A simple elastic membrane‐based microfluidic chip for the proliferation and differentiation of mesenchymal stem cells under tensile stress

A simple elastic membrane‐based microfluidic chip for the proliferation and differentiation of mesenchymal stem cells under tensile stress

Two-stage depth filter perfusion culture for recombinant antibody production by recombinant Chinese hamster ovary cell

Dynamic Stimulations with Bioengineered Extracellular Matrix‐Mimicking Hydrogels for Mechano Cell Reprogramming and Therapy

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