Effects of melatonin on Leydig cells in pinealectomized rat: an immunohistochemical study

Kuş, İlter; Akpolat, Nusret; Özen, Oğuz Aslan; Songür, Ahmet; Kavakli, Ahmet; Sarsılmaz, Mustafa

doi:10.1078/0065-1281-00618

Cited by 20 publications

(7 citation statements)

References 24 publications

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“…The originality of the present study is based on our investigation and confirmation of both the damaging effect of pulsed digital EMF as well as the neuroprotective effects of Mel and ω3 s on the EMF-exposed hippocampal pyramidal neurons and granule cell numbers in the DG. Mel, which is secreted from the pineal gland, is a direct radical scavenger and antioxidant [ 67 ]. Since it is highly lipophilic, Mel can be easily transferred to all tissues.…”

Section: Discussionmentioning

confidence: 99%

“…Another study showed that Mel significantly decreased brain damage caused by ischemic reperfusion. Thus, the antioxidant efficiency of Mel and its free radical scavenging activities are undeniable [ 67 69 ]. A study by Sutcu et al found that in addition to the anticancer and antioxidant activity of Mel, it also increased the NMDA (N-methyl- D -aspartic acid) receptor activity in the hippocampus [ 70 ].…”

Section: Discussionmentioning

confidence: 99%

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Protective effects of melatonin and omega-3 on the hippocampus and the cerebellum of adult Wistar albino rats exposed to electromagnetic fields

Altun

Kaplan

Deniz

et al. 2017

J Microsc Ultrastruct

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The purpose of the study was to investigate the effects of pulsed digital electromagnetic radiation emitted by mobile phones on the central nervous system of the adult Wistar albino rats. The study evaluated structural and functional impacts of four treatment arms: electromagnetic field (EMF) exposed; EMF exposed + melatonin treated group (EMF + Mel); EMF exposed + omega-3 (ω3) treated group (EMF + ω3); and control group (Cont). The 12-weeks-old rats were exposed to 900 MHz EMF for 60 min/day (4:00–5:00 p.m.) for 15 days. Stereological, biochemical and electrophysiological techniques were applied to evaluate protective effects of Mel and ω3. Significant cell loss in the CA1 and CA2 regions of hippocampus were observed in the EMF compared to other groups (p < 0.01). In the CA3 region of the EMF + ω3, a significant cell increase was found compared to other groups (p < 0.01). Granular cell loss was observed in the dentate gyrus of the EMF compared to the Cont (p < 0.01). EMF + ω3 has more granular cells in the cerebellum than the Cont, EMF + Mel (p < 0.01). Significant Purkinje cell loss was found in the cerebellum of EMF group compared to the other (p < 0.01). EMF + Mel and EMF + ω3 showed the same protection compared to the Cont (p > 0.05). The passive avoidance test showed that entrance latency into the dark compartment was significantly shorter in the EMF (p < 0.05). Additionally, EMF had a higher serum enzyme activity than the other groups (p < 0.01). In conclusion, our analyses confirm that EMF may lead to cellular damage in the hippocampus and the cerebellum, and that Mel and ω3 may have neuroprotective effects.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Protective effects of melatonin and omega-3 on the hippocampus and the cerebellum of adult Wistar albino rats exposed to electromagnetic fields

Altun

Kaplan

Deniz

et al. 2017

J Microsc Ultrastruct

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“…following pinealectomy could be reversed by Mel administration (Kuş et al, 2002). Previous studies also suggested that melatonin may play a role in suppressing the synthesis or release of testosterone, either indirectly via the hypothalamohypophysial axis (Hattori et al, 1995;McGuire et al, 2011), or directly at the gonadal level by inhibiting the synthesis of testosterone in the testes (Ahmad and Haldar, 2010).…”

Section: Figurementioning

confidence: 96%

Inhibitory effect of melatonin on testosterone synthesis is mediated via GATA-4/SF-1 transcription factors

Qin

Zhang

Liu

et al. 2015

Reproductive BioMedicine Online

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The aim of the present study was to elucidate whether the GATA-4/SF-1 signalling pathway is involved in the inhibitory effects of melatonin on testosterone production in both the TM3 Leydig cell line and in C57BL/6J mice. In-vitro experiments demonstrated that melatonin treatment significantly reduced testosterone levels in cell culture medium (P < 0.05 or P < 0.01); and decreased intracellular cyclic adenosine monophospha accumulation (P < 0.05 or P < 0.01) and mRNA/protein expression of GATA-4, SF-1 (NR5A1), StAR, P450SCC (CYP11A1) and 3β-HSD (P < 0.05 or P < 0.01). These effects were blocked by N-acetyl-2-benzyltryptamin, a melatonin receptor antagonist. Similar effects of melatonin on testosterone production (P < 0.05 or P < 0.01) and down-regulation of transcription factors GATA-4 and SF-1 (P < 0.01) were also observed in mice treated with intratesticular injections of melatonin. Overall, the data suggest that the inhibitory effects of melatonin on testosterone production are mediated via down-regulation of GATA-4 and SF-1 expression.

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“… 25 Studies have shown that MT could regulate a variety of physiological processes which include endocrine rhythms, 26 reproductive cycle, 27 immunomudulatory and vasomotor effects. 28 MT is able to prevent oxidative stress through its free radical scavenging effect and by directly increasing other antioxidant activities. 29 Furthermore, several MT metabolites which are formed when it neutralizes damaging reactants are themselves free radical scavengers.…”

Section: Introductionmentioning

confidence: 99%

Melatonin and N- Acetylcysteine as Remedies for Tramadol-Induced Hepatotoxicity in Albino Rats

Adikwu¹,

Bokolo²

2017

Adv Pharm Bull

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Purpose: The therapeutic benefit derived from the clinical use of tramadol (TD) has been characterized by hepatotoxicity due to misuse and abuse. The implications of drug-induced hepatotoxicity include socio-economic burden which makes the search for remedy highly imperative. The present study investigated the protective effects of melatonin (MT) and n-acetylcysteine (NAC) on TD-induced hepatotoxicity in albino rats. Methods: Forty five adult rats used for this study were divided into nine groups of five rats each. The rats were pretreated with 10mg/kg/day of NAC, 10mg/kg/day of MT and combined doses of NAC and MT prior to the administration of 15 mg/kg/day of TD intraperitoneally for 7 days respectively. At the termination of drug administration, rats were weighed, sacrificed, and serum was extracted and evaluated for liver function parameters. The liver was harvested, weighed and evaluated for oxidative stress indices and liver enzymes. Results: Alanine aminotransferase, alkaline phosphatase, aspartate aminotransferase, total bilirubin, conjugated bilirubin, and malondialdehyde levels were significantly (P<0.05) increased in rats administered with TD when compared to control. Furthermore, glutathione, superoxide dismutase and catalase levels were decreased significantly (P<0.05) in rats administered with TD when compared to control. The Liver of TD-treated rats showed necrosis of hepatocytes. However, the observed biochemical and liver histological alterations in TD-treated rats were attenuated in NAC and MT pretreated rats. Interestingly, pretreatment with combined doses of NAC and MT produced significant (P<0.05) effects on all evaluated parameters in comparison to their individual doses. Conclusion: Based on the findings in this study, melatonin and n- acetylcysteine could be used clinically as remedies for tramadol associated hepatotoxity.

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Effects of melatonin on Leydig cells in pinealectomized rat: an immunohistochemical study

Cited by 20 publications

References 24 publications

Protective effects of melatonin and omega-3 on the hippocampus and the cerebellum of adult Wistar albino rats exposed to electromagnetic fields

Protective effects of melatonin and omega-3 on the hippocampus and the cerebellum of adult Wistar albino rats exposed to electromagnetic fields

Inhibitory effect of melatonin on testosterone synthesis is mediated via GATA-4/SF-1 transcription factors

Melatonin and N- Acetylcysteine as Remedies for Tramadol-Induced Hepatotoxicity in Albino Rats

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