Effects of melatonin on microtubule assembly depend on hormone concentration: Role of melatonin as a calmodulin antagonist

Huerto-Delgadillo, Lourdes; Antón‐Tay, Fernando; Benítez‐King, Gloria

doi:10.1111/j.1600-079x.1994.tb00114.x

Cited by 118 publications

(92 citation statements)

References 30 publications

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“…In addition to its OFR scavenging properties, melatonin interacts with calmodulin (Benitez-King and Anton-Tay, 1993) microtubular components (Benitez-King and Anton-Tay, 1993) and is reported to increase the activity of the intrinsic cellular antioxidant defenses (Huerto-Delgadillo et al, 1994). Second, the bioavailability of this hormone makes it an ideal candidate for use in therapy.…”

Section: Discussionmentioning

confidence: 99%

Melatonin Prevents Death of Neuroblastoma Cells Exposed to the Alzheimer Amyloid Peptide

et al. 1997

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Studies from several laboratories have generated evidence suggesting that oxidative stress is involved in the pathogenesis of Alzheimer's disease (AD). The finding that the amyloid ␤ protein (A␤) has neurotoxic properties and that such effects are, in part, mediated by free radicals has provided insights into mechanisms of cell death in AD and an avenue to explore new therapeutic approaches. In this study we demonstrate that melatonin, a pineal hormone with recently established antioxidant properties, is remarkably effective in preventing death of cultured neuroblastoma cells as well as oxidative damage and intracellular Ca 2ϩ increases induced by a cytotoxic fragment of A␤. The effects of melatonin were extremely reproducible and corroborated by multiple quantitative methods, including cell viability studies by confocal laser microscopy, electron microscopy, and measurements of intracellular calcium levels. The importance of this finding is that, in contrast to conventional antioxidants, melatonin has a proposed physiological role in the aging process. Secretion levels of this hormone are decreased in aging and more severely reduced in AD. The reported phenomenon may be of therapeutic relevance in AD.

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Section: Discussionmentioning

confidence: 99%

Melatonin Prevents Death of Neuroblastoma Cells Exposed to the Alzheimer Amyloid Peptide

et al. 1997

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“…28,29 Several lines of evidence suggest that melatonin may act as a protective agent in ocular conditions such as photokeratitis, cataract, glaucoma, retinopathy of prematurity, and ischemia/reperfusion injury (for a review see 30 ). Besides its antioxidant effect, several other mechanisms are considered to be involved in neuroprotection induced by melatonin, including its interaction with calmodulin 31 and microtubular components, 32 blockade of intracellular Ca 2ϩ levels increase, 33 inhibition of the nitridergic pathway, 34,35 decrease in vascular endothelial growth factor levels, 36,37 and decrease in retinal glutamate synaptic concentrations, 38 among others. In this context, the aim of the present work was to analyze the therapeutic effect of melatonin in the golden hamster EIU.…”

Section: Uveitis Is a Common Ophthalmic Disorder That Can Be Induced mentioning

confidence: 99%

Therapeutic Effect of Melatonin in Experimental Uveitis

Sande

Fernandez

Marcos

et al. 2008

The American Journal of Pathology

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Uveitis is a common ophthalmic disorder that can be induced in hamsters by a single intravitreal injection of bacterial lipopolysaccharide (LPS). To examine the therapeutic effects of melatonin on uveitis, a pellet of melatonin was implanted subcutaneously 2 hours before the intravitreal injection of either vehicle or LPS. Both 24 hours and 8 days after the injection, inflammatory responses were evaluated in terms of i) the integrity of the blood-ocular barrier, ii) clinical signs, iii) histopathological studies, and iv) retinal function. Melatonin reduced the leakage of proteins and cells in the anterior segment of LPS-injected eyes, decreased clinical signs such as dilation of the iris and conjunctival vessels, and flare in the anterior chamber, and protected the ultrastructure of the blood-ocular barrier. A remarkable disorganization of rod outer segment membranous disks was observed in animals injected with LPS, whereas no morphological changes in photoreceptor outer segments were observed in animals treated with melatonin. Furthermore, melatonin prevented a decrease in LPS-induced electroretinographic activity. In addition, melatonin significantly abrogated the LPS-induced increase in retinal nitric-oxide synthase activity, tumor necrosis factor-␣, and nuclear factor B p50 and p65 subunit levels. These results indicate that melatonin prevents the clinical, biochemical, histological, ultrastructural, and functional consequences of experimental uveitis, likely through a nuclear factor B-dependent mechanism, and support the use of melatonin as a new therapeutic strategy for the treatment of uveitis. (Am J Pathol

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“…Melatonin is a calmodulin antagonist that prevents myosin phosphorylation, and thus vascular smooth muscle contraction, by binding to the calcium-calmodulin complex at the intracellular level. 32) The two melatonin receptor subtypes that have been identified in mammals have been designated as MT1 (1a) and MT2 (1b). 33) Decreased MT2 immunoreactivity and increased MT1 immunoreactivity have been reported in the hippocampus of AD patients.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Circadian Melatonin Levels on Inflammation and Neurocognitive Functions Following Coronary Bypass Surgery

Geyik

Yıgıter

Akcali

et al. 2015

ATCS

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Purpose: In this study, the relationship between the plasma levels of melatonin and intercellular adhesion molecule-1 (ICAM-1), which plays role in several intercellular interactions including inflammatory and immune responses, and early neurocognitive functions associated with ischaemia-reperfusion injury during open heart surgery is examined. Methods: Forty patients who were to undergo elective coronary artery bypass grafting (CABG) were divided into two groups, those who underwent their operations at 8 AM (group I; n = 20) and those who underwent their operations at 1 PM (group II; n = 20). Blood samples were collected prior to surgery (S1), when the aortic cross clamp was removed (S2) and 4 (S3) and 24 h after the surgery (S4). Neuropsychiatric assessment was conducted one day before and seven days after surgery. Results: Melatonin levels measured during and after surgery were also significantly higher in Group 1. ICAM-1 levels were significantly lower in Group 1 at S2 and S3. Significant deterioration was observed in postoperative neurocognitive function compared with preoperative functions in Group 2 more than Group 1. Conclusion: We hypothesise that the greater preservation of neurocognitive functions in the morning patients is associated with elevated melatonin levels, which reduce the damage from ischaemia-reperfusion injury.

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Effects of melatonin on microtubule assembly depend on hormone concentration: Role of melatonin as a calmodulin antagonist

Cited by 118 publications

References 30 publications

Melatonin Prevents Death of Neuroblastoma Cells Exposed to the Alzheimer Amyloid Peptide

Melatonin Prevents Death of Neuroblastoma Cells Exposed to the Alzheimer Amyloid Peptide

Therapeutic Effect of Melatonin in Experimental Uveitis

The Effect of Circadian Melatonin Levels on Inflammation and Neurocognitive Functions Following Coronary Bypass Surgery

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