Effects of melatonin supplementation on the quality of cryopreserved sperm in the neotropical fish Prochilodus lineatus

Motta, Naiara Cristina; Egger, Renata Catão; Monteiro, Kianne Silva; Oliveira, Alexmiliano Vogel de; Murgas, Luís David Solis

doi:10.1016/j.theriogenology.2021.11.012

Cited by 13 publications

(3 citation statements)

References 40 publications

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“…In this study, MT supplementation (0.25 mg/mL) significantly increased TM, while the addition of 0.1 mg/mL MT significantly increased VCL and VSL. Consistently with this, a previous study of Prochilodus lineatus showed that the addition of 2 mM (0.46 mg/mL) MT could significantly improve the VCL and VAP of frozen-thawed sperm, but did not change PM and VSL [43]. This result may be due to the ROS-scavenging effect of melatonin.…”

Section: Discussionsupporting

confidence: 82%

Supplementation of Extender with Melatonin Improves the Motility, Mitochondrial Membrane Potential, and Fertilization Ability of Cryopreserved Brown-Marbled Grouper Sperm

Ruan,

Yang,

Hua

et al. 2024

Animals

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Sperm cryopreservation is a valuable tool for breeding, conservation, and genetic improvement in aquatic resources, while oxidative damage will cause a decline in sperm quality during this progress. Melatonin (MT), a natural antioxidant hormone, is used as an additive in sperm cryopreservation to reduce cellular damage from oxidative stress. Here, we aimed to investigate the effect of adding MT to the freezing medium in sperm cryopreservation of brown-marbled grouper (Epinephelus fuscoguttatus). Different concentrations of MT (0, 0.1, 0.25, and 0.5 mg/mL) were tested. We evaluated sperm motility, viability, apoptosis, mitochondrial membrane potential (MMP), and fertilization ability to assess the effects of MT supplementation. Our results demonstrated that the addition of MT to the extender improved the post-thaw motility, MMP, and fertilization ability of brown-marbled grouper sperm. The total motility, curvilinear velocity, straight linear velocity, and average path velocity in MT-treated groups (0.1 and 0.25 mg/mL) exhibited significantly higher values than that of the control group. A higher MMP (p < 0.05) was observed in the group treated with 0.25 mg/mL MT, suggesting that supplementation of MT in the extender might be able to protect mitochondrial membrane integrity effectively. Regarding fertilizing ability, 0.25 mg/mL MT yielded a significantly higher hatching rate than the control. An adverse effect was found with the concentration of MT up to 0.5 mg/mL, suggesting the possible toxicity of a high-dose addition. In this study, we optimized the sperm cryopreservation protocol of brown-marbled grouper, which might be valuable for sperm cryopreservation and sample commercialization of groupers and other fish.

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Section: Discussionsupporting

confidence: 82%

Supplementation of Extender with Melatonin Improves the Motility, Mitochondrial Membrane Potential, and Fertilization Ability of Cryopreserved Brown-Marbled Grouper Sperm

Ruan,

Yang,

Hua

et al. 2024

Animals

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Section: Introductionmentioning

confidence: 99%

“…It has been reported that the addition of melatonin to a cryoprotectant or medium improves the viability and fertility of germ cells, but no studies have been conducted on the cryoprotective ability of post-thaw oocytes after the introduction of melatonin into the cells. [44][45][46][47] Here, we treated damaged oocytes with melatonin alone or melatonin-loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) to relieve the loss of oocyte quality due to mitochondrial and DNA damage, which can occur during oocyte maturation (Scheme 1). In previous studies, we conducted a study on the delivery of extracellular molecules into oocytes.…”

Section: Introductionmentioning

confidence: 99%