Effects of mesoporous silica nanoparticles upon the function of mammalian sperm in vitro

Barkalina, Natalia; Jones, Céline; Kashir, Junaid; Coote, Siobhan; Huang, Xinyue; Morrison, Rachel R.; Townley, Helen E.; Coward, Kevin

doi:10.1016/j.nano.2013.10.011

Cited by 61 publications

(45 citation statements)

References 44 publications

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“…[25][26][27] For example, our group has recently demonstrated the ability of MSNPs to bind with mammalian sperm without negative effects upon key semen parameters. 22 In addition, customization of the aforementioned nanoparticles with a cell-penetrating peptide coating has been shown to increase the rates of association with sperm. 28 Such developments in the utilization of nanodevices in reproductive medicine are emerging as powerful research tools as well as platforms for potential simultaneous diagnostic and therapeutic ("theranostic") applications.…”

Section: Discussionmentioning

confidence: 99%

“…Since MSNPs are distinguished for their low toxicity even in gametes, they represent a strong candidate for prospective clinical and research applications. 3,6,22 Nonetheless, an ongoing challenge for MSNP delivery lies in the efficiency of cargo release. A prominent limitation of current stimulienhanced MSNP systems is that they require complicated synthesis or assembly and nonsilica components that do not possess the same levels of biocompatibility.…”

Section: Discussionmentioning

confidence: 99%

“…For a detailed description of MSNP synthesis, characterization, and functionalization, refer to Barkalina et al 22 All synthesized MSNPs used in these experiments were functionalized with polyethileneimine (PEI, MW 1.3 kD, SigmaAldrich, UK) to impart a positive charge to the surface. For confocal microscopy, MSNPs were functionalized with both PEI and fluorescein isothiocyanate (FITC) to allow fluorescent tracking.…”

Section: Synthesis Of Mesoporous Silica Nanoparticlesmentioning

confidence: 99%

“…Loading of HSP:GFP plasmid onto PEI-coated MSNPs was achieved via electrostatic interaction between the negatively charged nucleic acid and the positively charged cationic surface of PEI-coated MSNPs, as previously described by Barkalina et al 22 Briefly, PEI-coated MSNPs were washed by centrifugation and redispersed in nuclease-free water (Ambion, UK). The cloned HSP:GFP plasmid was added to the resulting MSNP at a ratio of 10∶1 μg MSNP∶μg DNA by mass, and the mixture was incubated for 24 h at 4°C with gentle rotation.…”

Section: Loading Plasmid Cargo Onto Mesoporous Silica Nanoparticlesmentioning

confidence: 99%

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Development of a laser-activated mesoporous silica nanocarrier delivery system for applications in molecular and genetic research

et al. 2016

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Coward, "Development of a laseractivated mesoporous silica nanocarrier delivery system for applications in molecular and genetic research," J. Biomed. Opt. 21(11), 115002 (2016), doi: 10.1117/1.JBO.21.11.115002. Abstract. Nanoparticles have revolutionized medical research over the last decade. One notable emerging area of nanomedicine is research developments in the reproductive sciences. Since increasing evidence indicates links between abnormal gene expression and previously unexplained states of infertility, there is a strong impetus to develop tools, such as nanoparticle platforms, to elucidate the pathophysiological mechanisms underlying such states. Mesoporous silica nanoparticles (MSNPs) represent a powerful and safe delivery tool for molecular and genetic investigations. Nevertheless, ongoing progress is restricted by low efficiency and unpredictable control of cargo delivery. Here, we describe for the first time, the development of a laser-activated MSNP system with heat-responsive cargo. Data derived from human embryonic kidney cells (HEK293T) indicate that when driven by a heat-shock promoter, MSNP cargo exhibits a significantly increased expression following infrared laser stimulus to stimulate a heat-shock response, without adverse cytotoxic effects. This delivery platform, with increased efficiency and the ability to impart spatial and temporal control, is highly useful for molecular and genetic investigations. We envision that this straightforward stimuli-responsive system could play a significant role in developing efficient nanodevices for research applications, for example in reproductive medicine. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Synthesis Of Mesoporous Silica Nanoparticlesmentioning

confidence: 99%

Section: Loading Plasmid Cargo Onto Mesoporous Silica Nanoparticlesmentioning

confidence: 99%

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Development of a laser-activated mesoporous silica nanocarrier delivery system for applications in molecular and genetic research

et al. 2016

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“…Some researchers have demonstrated biocompatibility with mammalian sperm for magnetic iron NPs , mesoporous silica NPs (Barkalina et al, 2014b), halloysite clay nanotubes and commercial nanopolymer-based transfectants (Campos et al, 2011a). In turn, alternative data regarding the reproductive toxicity of most commonly used nanostructures, such as AuNPs, AgNPs and titanium dioxide (TiO 2 ) NPs, remain highly contradictory.…”

Section: Risk Of the Use Of Nanomaterialsmentioning

confidence: 99%