Effects of methotrexate and salazosulfapyridine on protein profiles of exosomes derived from a human synovial sarcoma cell line of SW982

Abstract

The SASP and/or MTX treatments altered the protein profiles of exosomes and suppressed the effects of IL-1β on the exosomal proteome. Exosomes may play roles in the actions of these anti-rheumatic drugs.

Exosomal amyloid A and lymphatic vessel endothelial hyaluronic acid receptor-1 proteins are associated with disease activity in rheumatoid arthritis

“…Changes in levels of specific inflammatory mediators during treatment have been assessed as predictors of treatment response [25, 26]. Treatment with MTX alters the protein profiles of exosomes, and MTX treatment suppresses some of the changes induced by IL-1β in vitro [27]. The protein and microRNA contents of exosomes have been suggested to reflect the physiological conditions of their cells of origin; exosomes also induce phenotypic changes in their origin cells in vitro [28].…”

Review: Extracellular Vesicles in Joint Inflammation

“…In one study of immortalized synovial cells, treatment with methotrexate and sulfasalazine induced changes in the protein content of released exosomes (52). In one study of immortalized synovial cells, treatment with methotrexate and sulfasalazine induced changes in the protein content of released exosomes (52).…”

The roles and implications of exosomes in sarcoma

“…The exosomes from human synovial sarcoma cell line SW982 cells were isolated through synthetic polymer-based precipitation and authenticated by their size (30-100nm) and positive exosomal-associated markers (ALIX and HSP70) through transmission electron microscopic analysis and Western blot [62]. The protein profiles of the exosomes from SW982 cells could be altered under the different influences from SASP (salazosulfapyridine) and MTX (methotrexate) respectively and synergistically [62]. Meanwhile, IL-1β was shown to not only influence the protein profiles of exosomes from SW982 cells, but also be inhibited by SASP and MTX [62].…”

“…The protein profiles of the exosomes from SW982 cells could be altered under the different influences from SASP (salazosulfapyridine) and MTX (methotrexate) respectively and synergistically [62]. Meanwhile, IL-1β was shown to not only influence the protein profiles of exosomes from SW982 cells, but also be inhibited by SASP and MTX [62]. Intriguingly, the protein content in exosomes was approximately one-third of that in SW982 cells, and the changes of protein profiles between the exosomes and SW982 cells were observed to be distinctly different after the SASP+MTX treatment, which implicated that the contents of exosomes are not a simple reproduction of the parental cells [62].…”

“…Meanwhile, IL-1β was shown to not only influence the protein profiles of exosomes from SW982 cells, but also be inhibited by SASP and MTX [62]. Intriguingly, the protein content in exosomes was approximately one-third of that in SW982 cells, and the changes of protein profiles between the exosomes and SW982 cells were observed to be distinctly different after the SASP+MTX treatment, which implicated that the contents of exosomes are not a simple reproduction of the parental cells [62]. More importantly, two functional groups of proteins were observed in exosomes named the immune system-related proteins, which include immune suppressors protein Rab-7b, protein Ran and SH2 domain containing protein 1B, and the oxidative stress-related proteins [62].…”