Effects of Methotrexate on Vascular Endothelial Growth Factor, Angiopoietin 1, and Angiopoietin 2 in Nasal Polyps

Park, Seong Kook; Kim, Hyeong In; Yang, Young Il; Hur, Dae Young

doi:10.2500/ajra.2011.25.3618

Cited by 15 publications

(22 citation statements)

References 23 publications

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“…With the increased VEGF expression, vascular permeability is increasing, the invasion of inflammatory cells, fibroblasts and endothelium cells would induce the formation of granulation tissue. Meanwhile, because of increased plasma extravasation, extracellular matrix accumulation and tissue edema, local increased force induces epithelial rupture, slowly forming nasal polyps [14,15]. In the current study, the positive expression of VEGF in nasal polyps was 75%, while low expression in inferior turbinate tissue, indicating the important role of VEGF in the development of nasal polyps.…”

Section: Discussionmentioning

confidence: 46%

The Expression and Significance of STAT6, VEGF and MVD in Nasal Polyps

Cao¹

2018

CRJ

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The aim of the current study was to evaluate the expression levels of STAT6, VEGF and microvessel density (MVD) score in nasal polyp tissues and to explore the underlying relationships during the pathogenesis of nasal polyps. 40 cases with surgical removal of nasal polyps were included, comparing with control group of 10 inferior turbinate mucosal tissues resected from nasal septum deviation correction surgery, from the same time period. By immunohistochemistry, the protein expression levels of STAT6, VEGF and MVD were determined. The results showed that the positive expression rate of STAT6 in mucosal epithelium and glands of nasal polyp tissues was 70%, which was significantly higher than that in control group (P < 0.01). The positive expression percentage of VEGF was 75%, which also higher than that in control group (P < 0.01). The MVD values in nasal polyp group was 22.67±7.54, while 7.04±2.63 in control group. The expression of STAT6 was closely related with the expression of VEGF and MVD, increasing with the level of VEGF and MVD. In general, the expression levels of STAT6, VEGF and MVD in nasal polyp group were increased and themselves were positively correlated, indicating the significant importance in the development of nasal polyp.

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Section: Discussionmentioning

confidence: 46%

The Expression and Significance of STAT6, VEGF and MVD in Nasal Polyps

Cao¹

2018

CRJ

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“…11 Another aspect is the overexpression of vascular endothelial growth factor (VEGF) in nasal polyps and its relevance for polyp formation that has gained increasing attention. 12 Previously, we and others reported about increased VEGF levels in nasal polyps 13,14 potentially associated with tissue edema, proliferation, and apoptosis. 15 Moreover, survivin up-regulation by VEGF is well known from several types of tissues.…”

mentioning

confidence: 98%

Dysregulated Survivin Expression in Nasal Polyps of Individuals with Aspirin Exacerbated Respiratory Disease

Fruth¹,

Schramek²,

Docter³

et al. 2012

Am J Rhinol�Allergy

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“…Moreover Methotrexate inhibits in vitro corneal vascular endothelial cell proliferation and blocks VEGF-and bFGF-induced corneal neovascularization in vivo (Hirata et al, 1989). Others also demonstrated significant decrease in serum levels of VEGF after 2 and 6 months of treatment with low dose methotrexate and cyclophosphamide in patients with metastatic breast cancer (Colleoni et al, 2002) .In addition, neoadjuvant multidrug chemotherapy including high-dose methotrexate modifies VEGF expression in osteosarcoma (Rossi et al, 2010;Park et al, 2011).…”

Section: Discussionmentioning

confidence: 98%

Novel DOX-MTX Nanoparticles Improve Oral SCC Clinical Outcome by Down Regulation of Lymph Dissemination Factor VEGF-C Expression in vivo: Oral and IV Modalities

Abbasi

Amir²,

Hamishehkar³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Background: Oral squamous cell carcinoma (OSCC) remains as one of the most difficult malignancies to control because of its high propensity for local invasion and cervical lymph node dissemination. The aim of present study was to evaluate the efficacy of novel pH and temperature sensitive doxorubicin-methotrexateloaded nanoparticles (DOX-MTX NP) in terms of their potential to change the VEGF-C expression profile in a rat OSCC model. Materials and Methods: 120 male rats were divided into 8 groups of 15 animals administrated with 4-nitroquinoline-1-oxide to induce OSCCs. Newly formulated doxorubicin-methotrexate-loaded nanoparticles (DOX-MTX NP) and free doxorubicin were IV and orally administered. Results: Results indicated that both oral and IV forms of DOX-MTX-nanoparticle complexes caused significant decrease in the mRNA level of VEGF-C compared to untreated cancerous rats (p<0.05) . Surprisingly, the VEGF-C mRNA was not affected by free DOX in both IV and oral modalities (p>0.05). Furthermore, in DOX-MTX NP treated group, less tumors characterized with advanced stage and VEGF-C mRNA level paralleled with improved clinical outcome (p<0.05). In addition, compared to untreated healthy rats , the VEGF-C expression was not affected in healthy groups that were treated with IV and oral dosages of nanodrug (p>0.05). Conclusions: VEGF-C is one of the main prognosticators for lymph node metastasis in OSCC. Down-regulation of this lymph-angiogenesis promoting factor is a new feature acquired in group treated with dual action DOX-MTX-NPs. Beside the synergic apoptotic properties of concomitant use of DOX and MTX on OSCC, DOX-MTX NPs possessed anti-angiogenesis properties which was related to the improved clinical outcome in treated rats. Taking together, we conclude that our multifunctional doxorubicin-methotrexate complex exerts specific potent apoptotic and anti-angiogenesis properties that could ameliorate the clinical outcome presumably via down-regulating dissemination factor-VEGF-C expression in a rat OSCC model.

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Effects of Methotrexate on Vascular Endothelial Growth Factor, Angiopoietin 1, and Angiopoietin 2 in Nasal Polyps

Abstract: MTX may inhibit the growth of NPs via local regulation of VEGF, Ang-1, and Ang-2 protein levels. We suggest that MTX can be used to treat NPs.

Cited by 15 publications

References 23 publications

The Expression and Significance of STAT6, VEGF and MVD in Nasal Polyps

The Expression and Significance of STAT6, VEGF and MVD in Nasal Polyps

Dysregulated Survivin Expression in Nasal Polyps of Individuals with Aspirin Exacerbated Respiratory Disease

Novel DOX-MTX Nanoparticles Improve Oral SCC Clinical Outcome by Down Regulation of Lymph Dissemination Factor VEGF-C Expression in vivo: Oral and IV Modalities

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