Effects of methyl-b12 on thein vitro immune functions of human T lymphocytes

Sakane, Tsuyoshi; Takada, S; Kotani, Hiroaki; Takemoto, Tsunematsu

doi:10.1007/bf00916893

Cited by 39 publications

(28 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Since such a correction of the CD4/CD8 ratio was also demonstrated in the present case of post-gastrectomy megaloblastic anemia, the phenomenon is considered to be not pathognomonic for PA but probably secondary to vitamin B12 deficiency. Interestingly, Sakane et al (7) reported that methyl-B12 enhances the induction of suppressor cells in in vitro concanavalin A-activated blood cell culture. Taken together, we propose the possibility that vitamin B12 deficiency in some cases causes a selective reduction in the blood suppressor cells which can be corrected by vitamin B12 supplement.…”

Section: Discussionmentioning

confidence: 99%

Restoration of Abnormally High CD4/CD8 Ratio and Low Natural Killer Cell Activity by Vitamin B12 Therapy in a Patient with Post-gastrectomy Megaloblastic Anemia.

et al. 1992

View full text Add to dashboard Cite

A patient with post-gastrectomy megaloblastic anemia is described. Initially, the CD4/CD8 ratio of blood cells was abnormally high and the level of natural killer cell activity was markedly low. These abnormalities were, however, restored by vitamin B12 treatment. Taken together with our prior report on a patient with pernicious anemia in whom the decreased level of CD8 positive blood cells was corrected by vitamin B12 therapy, it may be considered that the reversible CD4/CD8 imbalance of blood cells noted in some cases of pernicious anemia is not pathognomonic for the disease but secondary to vitamin B12 deficiency. (Internal Medicine 31: 125-126, 1992)

show abstract

Section: Discussionmentioning

confidence: 99%

Restoration of Abnormally High CD4/CD8 Ratio and Low Natural Killer Cell Activity by Vitamin B12 Therapy in a Patient with Post-gastrectomy Megaloblastic Anemia.

et al. 1992

View full text Add to dashboard Cite

show abstract

“…Cows' milk contains proteins, Ca and an abundance of vitamin B 12 . In particular, vitamin B 12 has been reported to augment the natural killer cell activity of patients with vitamin B 12 -deficiency anaemia and to enhance the T cell proliferative response to Con A (26,27) . In addition, recently, a relationship between vitamin B 12 status and the risk of cognitive impairment and dementia has been noted (28 -30) .…”

Section: Discussionmentioning

confidence: 99%

Reducing the risk of infection in the elderly by dietary intake of yoghurt fermented withLactobacillus delbrueckiissp.bulgaricusOLL1073R-1

Makino¹,

Ikegami²,

Kume³

et al. 2010

Br J Nutr

211

181

View full text Add to dashboard Cite

Immune senescence potentially leads to an increased risk of infections. It is desirable to augment the immune system and protect against infections by daily consumption of immunostimulatory food. The present study evaluated whether the intake of yoghurt fermented with Lactobacillus delbrueckii ssp. bulgaricus (L. bulgaricus) OLL1073R-1 has an effect on resistance to the common cold. We conducted two independent studies, in which fifty-seven (median age 74·5 years) and eighty-five healthy elderly individuals (median age 67·7 years) were participants. In each study, the subjects were divided into two groups based on age and sex and instructed to eat 90 g yoghurt or drink 100 ml milk once per d over an 8-or 12-week period. A meta-analysis of the results of these two independent studies showed the risk of catching the common cold was about 2·6 times lower (OR 0·39; P¼ 0·019) in the yoghurt group than in the milk group and the increase of natural killer cell activity was significantly higher in the yoghurt group than in the milk group (P¼0·028). In addition, the quality of life score for the 'eye/nose/throat' system after intake was significantly higher in the yoghurt group than in the milk group and the improvement of the score was correlated with the promotion of natural killer cell activity. In conclusion, consumption of yoghurt fermented with L. bulgaricus OLL1073R-1 augmented natural killer cell activity and reduced the risk of catching the common cold in elderly individuals. The proportion and absolute number of individuals above the age of 65 years are steadily increasing in most countries, particularly in industrialised nations. It is recognised that elderly individuals suffer from not only common age-related illness, including atherosclerosis, Alzheimer's dementia, diabetes mellitus and osteoporosis, but more frequent and more severe infections than younger individuals. The reasons for this include epidemiological elements, immunosenescence and malnutrition, as well as a large number of age-associated physiological and anatomical alterations (1)

show abstract

“…In order to examine the effects of vit.B12, methyl-B12 was used, because mecobalamin is known to be one of the strongest immunomodulators among the vit.B12 derivatives [1]. Methyl-B12 restored the abnormally high CD4/CD8 ratio and significantly decreased the number of CD8 þ cells.…”

mentioning

confidence: 91%

Immunomodulation by vitamin B12: augmentation of CD8+ T lymphocytes and natural killer (NK) cell activity in vitamin B12-deficient patients by methyl-B12 treatment

Tamura

Kubota

Murakami

et al. 1999

Clinical and Experimental Immunology

242

154

View full text Add to dashboard Cite

SUMMARYIt has been suggested that vitamin B12 (vit.B12) plays an important role in immune system regulation, but the details are still obscure. In order to examine the action of vit.B12 on cells of the human immune system, lymphocyte subpopulations and NK cell activity were evaluated in 11 patients with vit.B12 deficiency anaemia and in 13 control subjects. Decreases in the number of lymphocytes and CD8 þ cells and in the proportion of CD4 þ cells, an abnormally high CD4/CD8 ratio, and suppressed NK cell activity were noted in patients compared with control subjects. In all 11 patients and eight control subjects, these immune parameters were evaluated before and after methyl-B12 injection. The lymphocyte counts and number of CD8 þ cells increased both in patients and in control subjects. The high CD4/CD8 ratio and suppressed NK cell activity were improved by methyl-B12 treatment. Augmentation of CD3 ¹ CD16 þ cells occurred in patients after methyl-B12 treatment. In contrast, antibody-dependent cell-mediated cytotoxicity (ADCC) activity, lectin-stimulated lymphocyte blast formation, and serum levels of immunoglobulins were not changed by methyl-B12 treatment. These results indicate that vit.B12 might play an important role in cellular immunity, especially relativing to CD8 þ cells and the NK cell system, which suggests effects on cytotoxic cells. We conclude that vit.B12 acts as an immunomodulator for cellular immunity.

show abstract

Effects of methyl-b12 on thein vitro immune functions of human T lymphocytes

Cited by 39 publications

References 14 publications

Restoration of Abnormally High CD4/CD8 Ratio and Low Natural Killer Cell Activity by Vitamin B12 Therapy in a Patient with Post-gastrectomy Megaloblastic Anemia.

Restoration of Abnormally High CD4/CD8 Ratio and Low Natural Killer Cell Activity by Vitamin B12 Therapy in a Patient with Post-gastrectomy Megaloblastic Anemia.

Reducing the risk of infection in the elderly by dietary intake of yoghurt fermented withLactobacillus delbrueckiissp.bulgaricusOLL1073R-1

Immunomodulation by vitamin B12: augmentation of CD8+ T lymphocytes and natural killer (NK) cell activity in vitamin B12-deficient patients by methyl-B12 treatment

Contact Info

Product

Resources

About