Effects of methyl jasmonate and salicylic acid on the production of tropane alkaloids and the expression of PMT and H6H in adventitious root cultures of Scopolia parviflora

Kang, Shin-Min; Jung, Hee‐Young; Kang, Young Min; Yun, Dae‐Jin; Bahk, Jung-Dong; Yang, Jae-Kyung; Choi, Myung-Suk

doi:10.1016/j.plantsci.2003.11.022

Cited by 155 publications

(102 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was found that the root growth decreased when compared with the control culture. In previous reports, MJ also inhibited the root growth in adventitious root cultures of Scopolia parviflora [15]. For Stemona alkaloids biosynthesis, the total alkaloid production of MJ treatments is shown in Figure 1 (b-c) and Table II (a-b).…”

Section: Resultssupporting

confidence: 53%

Effects of Methyl Jasmonate and Salicylic Acid on Alkaloid Production from in vitro Culture of Stemona sp.

Chaichana¹,

Dheeranupattana²

2012

IJBBB

View full text Add to dashboard Cite

Abstract-Plant tissue culture technique combined with elicitors is required to produce plant secondary metabolites. The results showed that both elicitors (methyl jasmonate and salicylic acid) could enhance the production of Stemona alkaloids in the Stemona sp. culture. Among these elicitors, salicylic acid was found to be the most effective elicitor. The plantlet which was elicited with 100 µM salicylic acid for 1 week showed an increase of 1′,2′-didehydrostemo-foline and stemofoline production at 1.69 fold and 1.61 fold higher than the control, respectively. IndexTerms-1′,2′-didehydrostemofoline, stemofoline, methyl jasmonate, salicylic acid.

show abstract

Section: Resultssupporting

confidence: 53%

Effects of Methyl Jasmonate and Salicylic Acid on Alkaloid Production from in vitro Culture of Stemona sp.

Chaichana¹,

Dheeranupattana²

2012

IJBBB

View full text Add to dashboard Cite

show abstract

“…The genetic transformations and root culturing were performed according to the method of Kang et al (2004a). Escherichia coli XLl-Blue MRF (Stratagene, La Jolla, CA) was used in DNA cloning experiments.…”

Section: Methodsmentioning

confidence: 99%

Enhanced production of tropane alkaloids in transgenic Scopolia parviflora hairy root cultures over-expressing putrescine N-methyl transferase (PMT) and hyoscyamine-6β-hydroxylase (H6H)

Kang

Park

Jiang

et al. 2011

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

show abstract

“…The results showed that the key genes could be regulated by MJ, and the production of TA variation was observed in the range of 96 h in wild A. acutangulus plant. According to previous reports, MJ has been reported to stimulate the secondary metabolites production in many species, such as TA accumulation in Scopolia parviflora (Kang & Jung 2004), phenylpropanoid in Atropa belladonna (Lee et al 1998) and camptothecin accumulation inCamptotheca acuminate (Seung & Sang 1998). MJ has also been proven to be an effective signaling molecule, which can strongly boost taxane biosynthesis in cultured Taxus cells (Wang et al 2001).…”

Section: Discussionmentioning

confidence: 95%

“…ture recently (Boitel-Conti et al 2000;Pitta-Alvarez et al 2000;Kang & Jung et al 2004). Elicitors can be subdivided into two categories: abiotic elicitors, such as metal ions and inorganic compounds, and biotic elicitors from fungi, bacteria, viruses, and chemicals (Zhao et al 2005).…”

Section: Introductionmentioning

confidence: 99%

The effects of elicitation on the expression of key enzyme genes and on production of tropane alkaloids in Anisodus acutangulus plant

Luo

Weng

et al. 2012

Biologia

View full text Add to dashboard Cite

Tropane alkaloids (TAs) including hyoscyamine, anisodamine, anisodine and scopolamine are widely used as anticholinergic agents, which act on the parasympathetic nervous system, and their clinical demands are increasing rapidly. Anisodus acutangulus is a solanaceous perennial plant that is endemic to China with good potential for TA production and classified as an endangered species in China. Therefore there is a need to improve the TA production in this resource plant by biology methods. In our research, three different important elicitors including methyl jasmonate (MJ), yeast elicitor (YE) and abscisic acid (ABA) were used to study their effects on TA production, and genes expressions in A. acutangulus plant under each treatment were examined. The results revealed that TA production was induced by all the three treatments. However, its quantity varies with inducers used. The effect induced by MJ and YE were significant, while the effect induced by ABA was not obviously. To our knowledge, this is the first report on the relationship between TA production and genes expression profile induced by different elicitors in cultivatedA. acutangulus plant, which should provide some useful information on how to improve TA production with the cultivated A. acutangulus plant in the near future.

show abstract

Effects of methyl jasmonate and salicylic acid on the production of tropane alkaloids and the expression of PMT and H6H in adventitious root cultures of Scopolia parviflora

Cited by 155 publications

References 23 publications

Effects of Methyl Jasmonate and Salicylic Acid on Alkaloid Production from in vitro Culture of Stemona sp.

Effects of Methyl Jasmonate and Salicylic Acid on Alkaloid Production from in vitro Culture of Stemona sp.

Enhanced production of tropane alkaloids in transgenic Scopolia parviflora hairy root cultures over-expressing putrescine N-methyl transferase (PMT) and hyoscyamine-6β-hydroxylase (H6H)

The effects of elicitation on the expression of key enzyme genes and on production of tropane alkaloids in Anisodus acutangulus plant

Contact Info

Product

Resources

About