Effects of methyl parathion on the growth, cell size, pigment and protein content of Chlorella protothecoides

Saroja, Gandhimathinathan; Bose, Salil

doi:10.1016/0143-1471(82)90158-1

Cited by 17 publications

(3 citation statements)

References 11 publications

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“…Our observation that filtrates recovered from cultures supplied with algicidal concentrations of the phenolics produced normal growth when inoculated with a fresh culture of C. vulgaris or S. This study also indicates that chlorophyll contents were greatly reduced with a concomitant rise in a/b ratio. This is in contrast to the reported inhibition of chlorophyll a compared to chlorophyll b in Chlorella protothecoides grown autotrophically in the presence of 10-30/xgmL -1 methyl parathion (Saroja and Bose, 1982). The decreased photosynthetic activity in the presence of nitrophenols is in close agreement with the reduced production of chlorophylls.…”

Section: Discussioncontrasting

confidence: 56%

Effects of phenolic compounds on growth and metabolic activities of Chlorella vulgaris and Scenedesmus bijugatus isolated from soil

1992

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Phenol and three nitrophenols (o-nitrophenol, m-nitrophenol, p-nitrophenol), commonly occurring pollutants in natural eco-systems, were tested for their toxic effects on soil isolates of Chlorella vulgaris and Scenedesmus bijugatus, growing under phototrophic, photoheterotrophic and heterotrophic conditions. The toxicity criteria included cell number, chlorophyll, total protein and carbohydrate content, 14CO2 uptake and in vivo nitrate reductase activity. Both C. vulgaris and S. bijugatus were sensitive to the pollutants when the cultures were grown under phototrophic or heterotrophic conditions. However, the toxicity was found reversed or alleviated upon photoheterotrophic growth of the cultures.Transmission electron microscope studies revealed various cytological abnormalities in C. vulgaris in the presence of phenolics at algistatic levels.

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Section: Discussioncontrasting

confidence: 56%

Effects of phenolic compounds on growth and metabolic activities of Chlorella vulgaris and Scenedesmus bijugatus isolated from soil

1992

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“…Induction of biochemical changes in Chlorella sp. was slight with 10 mg L −1 methyl parathion, moderate with 20 mg L −1 and severe with 30 mg L −1 (Saroja and Bose 1982). Our results proved that C. mexicana was able to tolerate high concentration of acephate and imidacloprid, and further research should be conducted on the biochemical and genetic aspects of insecticide degradation by C. mexicana.…”

Section: Removal Of Acephate and Imidacloprid By C Mexicanasupporting

confidence: 63%

Insecticides induced biochemical changes in freshwater microalga Chlamydomonas mexicana

Kumar

Kabra

Min

et al. 2015

Environ Sci Pollut Res

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The effect of insecticides (acephate and imidacloprid) on a freshwater microalga Chlamydomonas mexicana was investigated with respect to photosynthetic pigments, carbohydrate and protein contents, fatty acids composition and induction of stress indicators including proline, superoxide dismutase (SOD) and catalase (CAT). C. mexicana was cultivated with 1, 5, 10, 15, 20 and 25 mg L(-1) of acephate and imidacloprid. The microalga growth increased with increasing concentrations of both insecticides up to 15 mg L(-1), beyond which the growth declined compared to control condition (without insecticides). C. mexicana cultivated with 15 mg L(-1) of both insecticides for 12 days was used for further analysis. The accumulation of photosynthetic pigments (chlorophyll and carotenoids), carbohydrates and protein was decreased in the presence of both insecticides. Acephate and imidacloprid induced the activities of superoxide dismutase (SOD) and catalase (CAT) and increased the concentration of proline in the microalga, which play a defensive role against various environmental stresses. Fatty acid analysis revealed that the fraction of polyunsaturated fatty acids decreased on exposure to both insecticides. C. mexicana also promoted 25 and 21% removal of acephate and imidacloprid, respectively. The biochemical changes in C. mexicana on exposure to acephate and imidacloprid indicate that the microalga undergoes an adaptive change in response to the insecticide-induced oxidative stress.

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“…As shown in Fig. 2, the initial cell growth rate in methanol was much slower than that in glucose due to the adaptation period [18,46].…”

Section: Consumption Of Both Co 2 and Methanol Under Mixotrophic Condmentioning

confidence: 95%

Effects of methanol on cell growth and lipid production from mixotrophic cultivation of Chlorella sp.

Choi

Seo

et al. 2011

Biotechnol Bioproc E

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The marine microalga Chlorella sp. was cultivated under mixotrophic conditions using methanol as an organic carbon source, which may also act to maintain the sterility of the medium for long-term outdoor cultivation. The optimal methanol concentration was determined to be 1% (v/v) for both cell growth and lipid production when supplying 5% CO 2 with 450 µE/m 2 /sec of continuous illumination. Under these conditions, the maximal cell biomass and total lipid production were 4.2 g dry wt/L and 17.5% (w/w), respectively, compared to 2.2 g dry wt/L and 12.5% (w/w) from autotrophic growth. Cell growth was inhibited at methanol concentrations above 1% (v/v) due to increased toxicity, whereas 1% methanol alone sustained 1.0 g dry wt/L and 4.8% total lipid production. We found that methanol was preferentially consumed during the initial period of cultivation, and carbon dioxide was consumed when the methanol was depleted. A 12:12 h (light:dark) cyclic illumination period produced favorable cell growth (3.6 g dry wt/L). Higher lipid production was observed with cyclic illumination than with continuous illumination (18.6% (w/w) vs 17.5% (w/w)), and better lipid production was also obtained under mixotrophic rather than autotrophic conditions. Interestingly, under mixotrophic conditions with 12:12 (h) cyclic illumination, high proportions of C 16:0 , C 18:0 , and C 18:1 were observed, which are beneficial for biodiesel production. These results strongly indicate that the carbon source is important for controlling both lipid composition and cell growth under mixotrophic conditions, and they suggest that methanol could be utilized to scale up production to an open pond type system for outdoor cultivation where light illumination changes periodically.

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Effects of methyl parathion on the growth, cell size, pigment and protein content of Chlorella protothecoides

Cited by 17 publications

References 11 publications

Effects of phenolic compounds on growth and metabolic activities of Chlorella vulgaris and Scenedesmus bijugatus isolated from soil

Effects of phenolic compounds on growth and metabolic activities of Chlorella vulgaris and Scenedesmus bijugatus isolated from soil

Insecticides induced biochemical changes in freshwater microalga Chlamydomonas mexicana

Effects of methanol on cell growth and lipid production from mixotrophic cultivation of Chlorella sp.

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