The ascorbic acid content of plants may vary widely depending upon the conditions under which the plants are grown, Maynard and Beeson (1944). I n recent work with tomatoes relatively great variations in ascorbic acid were found correlated with the season and location at which the plants were grown, while little influence of the supply to the plant of macronutrient and micronutrient elements was apparent [ Hamner, Lyon, and Kamner (1942) and Lyon, Beeson, and Ellis (1943)l. The work of many investigators [Hamner and Parks (1944) ; Reder, Ascham and Eheart (1943) ; Reid (1941, 1942) ; and Murphy (1942) ] with numerous plants has indicated that such climatic environmental factors as air temperature, light intensity, length of day, and rainfall have an influence on ascorbic acid values. On the other hand, there is much disagreement among investigators as to whether o r not soil conditions and fertilizer practices exert any effect.A study of the ascorbic acid content of pineapple fruit in Hawaii was thought to be profitable, especially since variations in the vitamin could be followed in conjunction with variations in climate and plant composition. Only one variety of pineapple is grown and all plants are vegetatively propagated so that genetic differences between plants are not of concern. Climatic differences from one location to another a few miles away are relatively great. Plants are grown under conditions of temperature that differ materially at elevations approximating sea level to others over 2,000 feet. Light intensities vary from 2,000 to 8,000 foot candles, depending upon season and location. Differences in rainfall may be very great. Fairly complete records of these climatic variables are kept for each field location. In addition, careful records of fertilizer practices are available. Studies on the chemical composition of the plants and the fruit are extensive. With all of these data available it was thought that variations in ascorbic acid might be correlated with some particular variable of climate or fertilizer practice, or that a correlation between the vitamin content and some other plant constituent might become apparent.
EXPERIMENTAL PROCEDUREThe ripe fruits from the experimental plots were harvested and processed to form juice in a commercial cannery. The samples of canned juice were shipped to the United States Nutrition Laboratory a t Ithaca, New York, and stored at 5.SoC.( 42OF.). The vitamin analyses were conducted as soon as possible thereafter.Hawaiian Pineapple Company, Honolulu, Hawaii.
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