Effects of Microbial Flora on Levels of Colony Stimulating Factor in Serums of Irradiated CFW Mice

Chang, Chienkuo F.; Pollard, Morris

doi:10.3181/00379727-144-37551

Cited by 16 publications

(3 citation statements)

References 6 publications

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“…The absence of the intestinal microflora in GF mice was associated with a reduction of the level and HU kill of both bone marrow and splenic CFU-GM. Regarding the difference in CFU-GM level between EC-SPF and GF mice, a reduced level has also been reported for GF CBA mice (6,21). In Swiss mice, on the other hand, no difference was observed between conventional and GF mice (2,22,23).…”

Section: Resultsmentioning

confidence: 89%

Myelopoiesis in experimentally contaminated specific-pathogen-free and germfree mice during oral administration of polymyxin

Goris¹,

Boer²,

Waaij³

1985

Infect Immun

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Oral administration of polymyxin to specific-pathogen-free C3H/Law mice which with previously contaminated with gram-negative bacteria resulted in complete suppression of cecal gram-negative bacteria. Suppression of cecal gram-negative bacteria was accompanied by reduction of the cecal endotoxin concentration from 10 to 1 microgram/g of cecal content as measured with a microtechnique for the Limulus amebocyte lysate assay. Endotoxin determination by this assay appeared to be unaffected by the amount of polymyxin present in cecal preparations after oral administration of this antibiotic. In experimentally contaminated specific-pathogen-free mice, the femoral concentration of progenitor cells forming granulocyte-macrophage colonies in vitro (CFU-GM) decreased significantly (P less than 0.001) to 66% of the initial control after 4 days of polymyxin treatment. However, the femoral CFU-GM concentration in germfree mice and splenic CFU-GM concentration in experimentally contaminated specific-pathogen-free and germfree mice was not affected by polymyxin treatment. The kinetic behavior of femoral and splenic CFU-GM in experimentally contaminated specific-pathogen-free and germfree mice was expressed as the in vivo sensitivity to the S-phase-specific cytostatic drug hydroxyurea, i.e., the hydroxyurea kill. Administration of polymyxin to experimentally contaminated specific-pathogen-free mice significantly diminished the hydroxyurea kill of femoral CFU-GM from 29 to 13% (P less than 0.02) and of splenic CFU-GM from 53 to 27% (P less than 0.005). The hydroxyurea kill of femoral CFU-GM in germfree mice was not significantly affected by polymyxin treatment. On basis of these results we conclude that the effect of polymyxin treatment on myelopoiesis is most likely due to elimination of intestinal gram-negative bacteria and may indicate a significant role of intestinal gram-negative bacteria in the regulation of myelopoiesis.

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Section: Resultsmentioning

confidence: 89%

Myelopoiesis in experimentally contaminated specific-pathogen-free and germfree mice during oral administration of polymyxin

Goris¹,

Boer²,

Waaij³

1985

Infect Immun

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“…It has been appreciated for decades that intestinal commensal bacteria are needed to shape the hematopoietic compartment and myeloid generative capacity of adult mice ( Fig. 2 ), as reductions in commensal bacteria, achieved either in GF mice or by oral administration of antibiotics, result in severe dysregulation of BM myelopoiesis ( Chang and Pollard, 1973 ; Staber et al, 1978 ; MacVittie and Walker, 1978 ; Joshi et al, 1979 ; Goris et al, 1985 ). In contrast, the cellularity of the spleen, thymus, and peripheral blood as well as the distribution of B and T lymphocytes in all organs remains unaffected ( Tada et al, 1996 ).…”

Section: Tonic Inflammatory Signaling In Adult Hematopoietic Homeostasismentioning

confidence: 99%

Inflammatory signaling regulates hematopoietic stem and progenitor cell development and homeostasis

Collins

Mitchell

Passegué

2021

Journal of Experimental Medicine

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Inflammation exerts multiple effects on the early hematopoietic compartment. Best studied is the role of proinflammatory cytokines in activating adult hematopoietic stem and progenitor cells to dynamically replenish myeloid lineage cells in a process known as emergency myelopoiesis. However, it is increasingly appreciated that the same proinflammatory signaling pathways are used in diverse hematopoietic scenarios. This review focuses on inflammatory signaling in the emergence of the definitive hematopoietic compartment during embryonic life, and tonic inflammatory signals derived from commensal microbiota in shaping the adult hematopoietic compartment in the absence of pathogenic insults. Insights into the unique and shared aspects of inflammatory signaling that regulate hematopoietic stem and progenitor cell function across the lifespan and health span of an individual will enable better diagnostic and therapeutic approaches to hematopoietic dysregulation and malignancies.

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“…Animals infected with gram-negative bacteria, such as Salmonella typhimurium, also show a rapid increase in serum CSF and a subsequent increase in numbers of mononuclear phagocytes (2,40). Additionally, it has been shown that increases in serum CSF after Xirradiation are associated with the presence of gram-negative bacteria in the intestinal tract (7), whereas conventionalization of antibiotic-pre-2^C TABLE 2. Macrophage colony formation by exudate progenitor cells.exposed to LPS or CSF at 0 or 24 h Addition to culture at:…”

Section: Discussionmentioning

confidence: 99%

Role of Lipopolysaccharide in Regulating Colony-Stimulating Factor-Dependent Macrophage Proliferation In Vitro

et al. 1980

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Bacterial lipopolysaccharides (LPS) enhance both production of colony-stimulating factors (CSF) and proliferation of mononuclear phagocytes in vivo. The present study was undertaken to determine whether the effects of LPS on CSF-dependent monopoiesis are due solely to enhanced production of CSF or also to direct effects of LPS on the responding progenitor cell. Addition of LPS to CSF-stimulated macrophage populations had different effects, depending upon the concentration of CSF in the cultures. In the presence of optimal to supraoptimal concentrations of CSF, LPS at doses ≥0.01 μg/ml inhibited macrophage colony formation. This inhibitory activity was not due to cytotoxicity of the LPS and was not mediated through prostaglandin synthesis. In the presence of suboptimal concentrations of CSF, minute concentrations of LPS (10 −7 μg/ml) significantly enhanced macrophage colony formation. Both effects of LPS (inhibition and enhancement) appeared to be properties of lipid A since neither effect was noted with cells from LPS-resistant C3H/HeJ mice, whereas both effects could be neutralized by the addition of the antibiotic polymyxin B, which binds to the lipid A portion of LPS. These results suggest that the effects of LPS on monopoiesis in vivo may not be due solely to its capacity to stimulate production of CSF. Rather, LPS may be involved in stimulating monopoiesis both indirectly through stimulation of CSF production and by its effects on the CSF-responsive progenitor cell.

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Effects of Microbial Flora on Levels of Colony Stimulating Factor in Serums of Irradiated CFW Mice

Cited by 16 publications

References 6 publications

Myelopoiesis in experimentally contaminated specific-pathogen-free and germfree mice during oral administration of polymyxin

Myelopoiesis in experimentally contaminated specific-pathogen-free and germfree mice during oral administration of polymyxin

Inflammatory signaling regulates hematopoietic stem and progenitor cell development and homeostasis

Role of Lipopolysaccharide in Regulating Colony-Stimulating Factor-Dependent Macrophage Proliferation In Vitro

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