Effects of microcurrent therapy on excisional elastic cartilage defects in young rats

Filho, Edson Pereira Tangerino; Fachi, José Luís; Vasconcelos, Israel Costa; Santos, Gláucia Maria Tech dos; Mendonça, Fernanda Aparecida Sampaio; Aro, Andréa Aparecida de; Pimentel, Edson Rosa; Esquisatto, Marcelo Augusto Marretto

doi:10.1016/j.tice.2016.03.004

Cited by 7 publications

(8 citation statements)

References 51 publications

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“…As the P50/S50 exhibited interconnected porous structure and higher level of cell growth compared to two other (P75/S25 and P25/S75) blended hydrogels, we selected the P50/S50 hydrogel for both in vitro and in vivo chondrogenesis studies. In our in vitro chondrogenesis study, the H&E and MT staining results indicated that the chondrocytes located within the newly formed cartilage matrix and immature chondrocyte lacunar structure ( Figure 6 ) had morphological characteristics similar to those of native cartilage tissue [ 57 , 58 ]. It was also observed that the number of lacuna increased from four weeks to six weeks ( Figure 6 ), indicating that cartilage maturity gradually increased over time.…”

Section: Discussionmentioning

confidence: 99%

Artificial Auricular Cartilage Using Silk Fibroin and Polyvinyl Alcohol Hydrogel

Lee

Sultan

Kim

et al. 2017

IJMS

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Several methods for auricular cartilage engineering use tissue engineering techniques. However, an ideal method for engineering auricular cartilage has not been reported. To address this issue, we developed a strategy to engineer auricular cartilage using silk fibroin (SF) and polyvinyl alcohol (PVA) hydrogel. We constructed different hydrogels with various ratios of SF and PVA by using salt leaching, silicone mold casting, and freeze-thawing methods. We characterized each of the hydrogels in terms of the swelling ratio, tensile strength, pore size, thermal properties, morphologies, and chemical properties. Based on the cell viability results, we found a blended hydrogel composed of 50% PVA and 50% SF (P50/S50) to be the best hydrogel among the fabricated hydrogels. An intact 3D ear-shaped auricular cartilage formed six weeks after the subcutaneous implantation of a chondrocyte-seeded 3D ear-shaped P50/S50 hydrogel in rats. We observed mature cartilage with a typical lacunar structure both in vitro and in vivo via histological analysis. This study may have potential applications in auricular tissue engineering with a human ear-shaped hydrogel.

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Section: Discussionmentioning

confidence: 99%

Artificial Auricular Cartilage Using Silk Fibroin and Polyvinyl Alcohol Hydrogel

Lee

Sultan

Kim

et al. 2017

IJMS

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“…These endogenous signals are compromised when an injury occurs, and the exogenous electrical stimulus can accelerate the regeneration process by propagating the current compensating the lack of endogenous electrical signals of the injured tissue, generating piezoelectric energy 45,46 that restores the electrical current. The literature shows in several tissues that low‐intensity electrostimulation (10 to 20 µA) is effective in this process 10,11,13‐16,45 . But according to Cheng et al, 18 currents higher than 50 µA are harmful to the repair as they promote protein denaturation.…”

Section: Discussionmentioning

confidence: 99%

“…The literature has demonstrated important effects of the microcurrent in increasing the synthesis and organization of collagen fibers during the healing of different tissues, such as the skin, 9‐11 bone, 13,14 and cartilage, 15,16 although there is no detailed description of the mechanisms involved. Our data demonstrate that the microcurrent increased the expression of genes related to the synthesis and organization of collagen, such as FMDO .…”

Section: Discussionmentioning

confidence: 99%

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Microcurrent and adipose‐derived stem cells modulate genes expression involved in the structural recovery of transected tendon of rats

et al. 2020

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Tendon injuries are common and have a high incidence of re-rupture that can cause loss of functionality. Therapies with adipose-derived stem cells (ASC) and the microcurrent (low-intensity electrical stimulation) application present promising effects on the tissue repair. We analyzed the expression of genes and the participation of some molecules potentially involved in the structural recovery of the Achilles tendon of rats, in response to the application of both therapies, isolated and combined. The tendons were distributed in five groups: normal (N), transected (T), transected and ASC (C) or microcurrent (M) or with ASC, and microcurrent (MC). Microcurrent therapy was beneficial for tendon repair, as it was observed a statistically significant increase in the organization of the collagen fibers, with involvement of the TNC, CTGF, FN, FMDO, and COL3A1 genes as well as PCNA, IL-10, and TNF-α. ASC therapy significantly increased the TNC and FMDO genes expression with no changes in the molecular organization of collagen. With the association of therapies, a significant greater collagen fibers organization was observed with involvement of the FMOD gene. The therapies did not affect the expression of COL1A1, SMAD2, SMAD3, MKX, and EGR1 genes, nor did they influence the amount of collagen I and III, caspase-3, tenomodulin (Tnmd), and hydroxyproline. In conclusion, the application of the microcurrent isolated or associated with ASC increased the organization of the collagen fibers, which can result in a greater biomechanical resistance in relation to the tendons treated only with ASC. Future studies will be needed to demonstrate the biological effects of these therapies on the functional recovery of injured tendons.

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“…A eletroestimulação é capaz de estimular o recrutamento de células inflamatórias para o local da lesão a fim de auxiliar no processo de cicatrização (MENDONÇA, et al, 2009;TANGERINO FILHO et al, 2016;YU et al, 2019), o que foi observado em nossos resultados que corrobora com outros estudos. Células inflamatórias realizam multitarefas no local lesionado, facilitando o desbridamento da lesão e produzindo quimiocinas, metabólitos e fatores de crescimento (EMING; WYNN; MARTIN, 2017).…”

Section: H Perforatum Modula a Inflamação E A Eletroestimulação Promove Angiogêneseunclassified

Lesões Incisionais Tratadas Com Hypericum Perforatum L. E Eletroestimulação

Esquisatto

2020

REP

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O presente trabalho estudou efeitos de Hypericum perforatum associado ou não à eletroestimulação na cicatrização da pele de ratos. Trinta e seis animais foram divididos em (n=9): (C) sem tratamento; (MC) tratado com eletroestimulação; (HYP) tratado com H. perforatum; (HYP+MC) tratado com H. perforatum+eletroestimulação. Amostras foram coletadas dos animais eutanasiados no 2º, 6º e 10º dia após a lesão cirúrgica para análise histomorfométrica: quantificação de infiltrado inflamatório, vasos sanguíneos, fibroblastos e porcentagem da área de colágeno. Para análise estatística foi utilizado ANOVA Two-way e pós-teste de Tukey (média±erro padrão). Os resultados mostraram que o infiltrado inflamatório foi menor em HYP e MC+HYP em relação a C e MC no 2º e 6º período experimental; no dia 10 a eletroestimulação promoveu aumento do número dessas células. Em relação aos vasos sanguíneos observou-se que MC apresentou maiores valores no 10º período experimental em relação aos demais grupos. No 2º e 6º dia após a lesão, MC+HYP mostrou maior número de fibroblastos em relação aos demais grupos experimentais, o mesmo ocorrendo no 6º dia em MC e HYP em relação a C. Quanto à porcentagem de área de colágeno observou-se aumento em HYP e MC+HYP no 2º dia em relação a C e MC. Diante disso, pode-se concluir que H. perforatum em formulação homeopática neste modelo experimental diminuiu inflamação, promoveu fibroplasia e colagênese. A eletroestimulação diminuiu inflamação e estimulou angiogênese; em associação ou não ao H. perforatum promoveu fibroplasia. Portanto, essas terapias podem ser indicadas como alternativas no reparo de lesões incisionais.

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Effects of microcurrent therapy on excisional elastic cartilage defects in young rats

Cited by 7 publications

References 51 publications

Artificial Auricular Cartilage Using Silk Fibroin and Polyvinyl Alcohol Hydrogel

Artificial Auricular Cartilage Using Silk Fibroin and Polyvinyl Alcohol Hydrogel

Microcurrent and adipose‐derived stem cells modulate genes expression involved in the structural recovery of transected tendon of rats

Lesões Incisionais Tratadas Com Hypericum Perforatum L. E Eletroestimulação

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