Effects of microplastics on head kidney gene expression and enzymatic biomarkers in adult zebrafish

Limonta, Giacomo; Mancia, Annalaura; Abelli, Luigi; Fossi, María Cristina; Caliani, Ilaria; Panti, Cristina

doi:10.1016/j.cbpc.2021.109037

Cited by 18 publications

(2 citation statements)

References 68 publications

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“…Consistent with that in mouse, Ca-CYP2J6 mRNA exhibits low expression level in head kidney, but presents the highest level in gill. As for CYP2P8, the findings have shown that in zebrafish, the lowest microplastic concentration could induce the activation of an immune response and the xenobiotic metabolism, resulting in cyp2p8 gene up-regulated in head kidney (Limonta et al, 2021), as the primary haematopoietic site and one of the largest lymphoid organs in teleost fish, together with the spleen, involved in blood filtering (Uribe et al, 2011). The Ca-CYP2P8 mRNA tissue expression pattern, with the highest level in gill, then in head kidney and blood, further identified this point.…”

Section: Cyp2 and Cyp3 Familiesmentioning

confidence: 99%

Transcriptomics‐based systematic identification and tissue‐specific distribution of cytochrome P450 genes in Carassius auratus

et al. 2022

Aquaculture Research

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Cytochrome P450 (CYP450) enzymes are superfamily with a terminal oxidase of the single-chain B group and iron protoporphyrin as a prosthetic group. Due to the similar structure to haem and an absorption peak at 450 nm after interacting with CO, this superfamily was named CYP450 enzyme. CYP450, as one of the important metabolic enzymes, is involved in the detoxification of exogenous chemicals such as drugs and environmental toxicity, and in the metabolism of endogenous substrates, such as steroids, polyunsaturated fatty acids and fat-soluble vitamins (Nebert et al., 2004). CYP450 enzyme system, an electron transport system, is constituted of flavin protein, haem protein and phospholipid, with flavin protein consisting of nicotinamide adenine dinucleotide phosphate (NADPH)-b5 and NADPH-P450 reductase, and haem protein containing b5 and P450.CYP450 gene superfamily (CYPs) is an ancient gene superfamily and is widely distributed in various organs and tissues of animals and plants (Liao et al., 2017). However, its distribution varies from species to species. In mammals, CYPs are most widely distributed in liver, kidney, brain, pancreas, skin, intestine and lung (Zhu & Lou, 2004).In fish, CYP450 is mainly expressed in liver, followed by intestine, kidney and gill (Itakura et al., 2005).CYPs genes are induced by polycyclic aromatic hydrocarbons (PAHs), pesticides and heavy metals. In fish, numerous studies of the CYP1A have demonstrated its utility as a biomarker for aquatic pollution. In Carassius auratus, it has been shown that phoxim, an organophosphorus pesticide, can induce the down-regulation of CYP1A and CYP3A messenger RNA (mRNA) expression levels in liver.

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Section: Cyp2 and Cyp3 Familiesmentioning

confidence: 99%

Transcriptomics‐based systematic identification and tissue‐specific distribution of cytochrome P450 genes in Carassius auratus

et al. 2022

Aquaculture Research

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“…It has been demonstrated that PS-NPs have the ability to translocate the mucosal tissues of blood, lymphatic circulation, and secondary organs (Rubio et al 2020), depending on the physicochemical properties of the particles, including size, chemical structure, and surface charge (Fournier et al 2020). PS-NPs may cause tissue dysfunction or damage where they persist, potentially leading to oxidative stress, metabolic disturbances, neurotoxicity, and immune dysfunction (Barboza et al 2018, Limonta et al 2021, Qiao et al 2019). Lu et al have demonstrated that exposure to PS-NPs results in an imbalance of intestinal homeostasis and impaired immune cytotoxicity, as well as a dysbiosis of the gut microbiota.…”

Section: Introductionmentioning

confidence: 99%

Polystyrene nanoplastic exposure enhances LPS-induced lung inflammation in mice by inducing M1 polarization of macrophages via ROS/JAK/STAT signaling pathway

Fan

Peng

et al. 2022

Preprint

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Nanoplastics (NPs) are an emerging environmental pollutant. NPs can spike various tissues results to oxidative stress and tissue damage in organisms. While recent studies have reported a relationship between nanoparticles and respiratory system injury, the specific mechanism of NP exposure-induced lung damage remains to be explored. In the present study, C57BL6 male mice were treated intraperitoneal injection of PS-NPs and/or LPS. The relevant indicators were detected by HE staining, western blotting and qRT-PCR. RAW264.7 was pretreated with JAK2 inhibitor (AG490) to verify whether the JAK2/STAT3 signaling pathway is involved in PS-NPs exposure enhances LPS-induced pulmonary inflammatory response. We found decreased antioxidant capacity in mice lungs, activation of the JAK2/STAT3 pathway, and the expression levels of macrophage M1 marker genes increased (including CD16, CD86, and MCP1), while macrophage M2 marker genes (including CD206, PPARγ, and Arg1) expression levels decreased, resulting in a macrophage M1/M2 imbalance. In addition, PS-NPs can increase the expression of inflammation-related factors IL-1β, TNF-α and IL-6. In in vitro experiments, we obtained similar results to in vivo experiments. More importantly, the JAK2 pathway inhibitor AG490 reversed ROS-induced changes in macrophage imbalance and inflammation in PS-NPs and LPS-exposed RAW264.7 cells. In conclusion, PS-NPs activated the ROS/JAK/STAT pathway, aggravated LPS-induced lung M1/M2 macrophage imbalance and promoted inflammatory responses. Our results enrich the toxic effects and related molecular mechanisms of NPs-induced lung inflammation, and provide new insights into the toxic effects of NPs on mammals.

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On the Novel Process of Pristine Microplastic Bio-fragmentation by Zebrafish (Danio rerio)

Khan¹,

Ali²

2023

Arch Environ Contam Toxicol

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Effects of microplastics on head kidney gene expression and enzymatic biomarkers in adult zebrafish

Cited by 18 publications

References 68 publications

Transcriptomics‐based systematic identification and tissue‐specific distribution of cytochrome P450 genes in Carassius auratus

Transcriptomics‐based systematic identification and tissue‐specific distribution of cytochrome P450 genes in Carassius auratus

Polystyrene nanoplastic exposure enhances LPS-induced lung inflammation in mice by inducing M1 polarization of macrophages via ROS/JAK/STAT signaling pathway

On the Novel Process of Pristine Microplastic Bio-fragmentation by Zebrafish (Danio rerio)

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