Effects of MicroRNA-143 in the differentiation and proliferation of bovine intramuscular preadipocytes

Abstract

The purpose of the present study was to investigate the regulation of propionate on the proliferation and differentiation of cultured intramuscular preadipocytes from three adult Luxi Yellow steers. The fl ow cytometry, lipid droplets stained by oil red O, and cytoplasmic fat content were detected after cultured cells were treated with different concentrations of propionate (0, 1, 3, 6 mM). The results showed that the separated cells were highly homogeneous and proliferative. Propionate (1 mM) did not signifi cantly affect (P>0.05) the cell proportion of phase G0/G1, but remarkably (P<0.05) increased the proportion of phase S+G2/M. However, the cell proportion signifi cantly increased (P<0.05) in the phase G0/G1, and decreased (P<0.05) in the phase S+G2/M when the concentration of propionate reached to 6 mM. When the confl uent intramuscular preadipocytes were treated with insulin (10 µg/ml) and dexamethasone (0.25 µM), small lipid droplets began to appear, and their number rapidly increased around the nuclei on day 6. Subsequently, when the confl uent intramuscular preadipocytes were treated with propionate, signifi cant accumulation of lipid droplets was observed on day 6, and lipid accumulation extent was dependent on the dose of propionate. In addition, cytoplasmic fat contents of cultured preadipocytes treated with propionate (1, 3, 6 mM) for 6 days were 1.5, 2, and 2 times higher than those of control group (without propionate addition). In conclusion, the present study confi rmed that there were indeed functionally active preadipocytes existing in the muscle of Chinese adult Luxi steers. Propionate may play an important physiological role in regulation of adipogenesis and stimulation of proliferation and differentiation of Luxi bovine intramuscular preadipocytes.

MicroRNAs in farm animals

“…Expression of miR-143 is upregulated during differentiation of bovine intramuscular preadipocytes into mature adipocytes. Transfection of miR-143 antisense oligonucleotide represses the differentiation of bovine intramuscular preadipocytes (Li et al, 2011b). These observations further support a role of miR-143 in intramuscular fat development in cattle.…”

Effects of miRNA-143 and the non-coding RNA MALAT1 on the pathogenesis and metastasis of HeLa cells

“…For the same duration of incubation, HeLa cells transfected with MALAT1 or the miRNA-143 inhibitor had elevated proliferative activity, and those transfected with miRNA-143 or the MALAT1 inhibitor showed lower proliferation, compared to that of the control transfected cells. These results suggested that the high levels of miRNA-143 and/or low levels of MALAT1 decreased the proliferation of HeLa cells, while low levels of miRNA-143 and high levels of MALAT1 might facilitate proliferation, consistent with previous studies revealing the negative regulation of miRNA-143 in cell proliferation (Li et al, 2011;Liu et al, 2016). Lajer et al (2012) suggested that miRNA-143 might participate in the pathogenesis of the disease in a way that is similar to that observed for human papilloma virus infection in patients with cervical cancer.…”

Chemerin inhibition of myogenesis and induction of adipogenesis in C2C12 myoblasts

“…The conversion of cells into adipocytes was confirmed by the detection of lipid droplets under microscopy using Oil Red O staining (Li et al, 2011). In briefly, cells were rinsed three times in PBS and then fixed in 4% (v/v) formaldehyde for 10 min, after which the fixed cells were rapidly rinsed with DPBS.…”