Effects of MicroRNA-27a on Myogenin Expression and Akt/FoxO1 Signal Pathway during Porcine Myoblast Differentiation

Zhang, Shurun; Chen, Xiaoling; Huang, Zhiqing; Chen, Shuai; Yu, Bing; He, Jun; Zheng, Ping; Yu, Jie; Luo, Junqiu; Luo, Yuheng; Chen, Hong

doi:10.1080/10495398.2017.1348357

Cited by 9 publications

(7 citation statements)

References 42 publications

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“…The modulation of FoxO1 involves in Akt (Zhang et al., 2018) and AMPK (Liu et al., 2018) pathways. We thus evaluated the expression of the proteins in these pathways.…”

Section: Resultsmentioning

confidence: 99%

“…FoxO1 has been reported to be modulated by Nrf2 and SIRT1 signaling pathways (Lee and Goldberg, 2013; Gille et al., 2019). FoxO1 is involved in various pathways, including Akt (Zhang et al., 2018) and AMPK (Liu et al., 2018) pathways. We demonstrated that propofol reversed the elevated phosphorylation level of Akt and AMPK pathways induced by OGD/R in H9c2 cells.…”

Section: Discussionmentioning

confidence: 99%

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Propofol Alleviates DNA Damage Induced by Oxygen Glucose Deprivation and Reperfusion via FoxO1 Nuclear Translocation in H9c2 Cells

et al. 2019

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Ischemia/reperfusion (I/R) injury induces irreversible oxidative stress damage to the cardiac myocytes. Many studies have revealed that propofol alleviates the important organelle-mediated injury from oxidative stress in vitro. However, it remains unclear whether propofol prevents I/R-induced DNA damage in cardiomyocytes. In our study, we established an oxygen glucose deprivation/reoxygenation (OGD/R) model in H9c2 cells and found that propofol decreased reactive oxygen species (ROS) levels and suppressed cell apoptosis induced by OGD/R in H9c2 cells. In addition, propofol significantly reduced the molecular marker of DNA damage and inhibited double-strand breaks of DNA damage induced by OGD/R in H9c2 cells in a dose-dependent manner. Furthermore, we investigated the molecular mechanisms and demonstrated that propofol inhibited forkhead box O 1 (FoxO1) phosphorylation and increased FoxO1 nuclear translocation through inhibition of protein kinase B (Akt) and adenosine 5’-monophosphate-activated protein kinase (AMPK) pathways. The protective effects of propofol against oxidative stress-induced DNA damage were reversed by silencing FoxO1. Taken together, our results suggest that oxidative stress aggravates DNA damage and apoptosis in H9C2 cells, which can be reversed by propofol via FoxO1 nuclear translocation.

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“…The modulation of FoxO1 involves in Akt (Zhang et al., 2018) and AMPK (Liu et al., 2018) pathways. We thus evaluated the expression of the proteins in these pathways.…”

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Propofol Alleviates DNA Damage Induced by Oxygen Glucose Deprivation and Reperfusion via FoxO1 Nuclear Translocation in H9c2 Cells

et al. 2019

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“…For example, treatment of melengestrol acetate increases myogenin mRNA expression in C2C12 cells, but no affect is observed in bovine muscle satellite cells ( 23 ) . miR-27a has the opposite role on differentiation between C2C12 cells and porcine myoblasts ( 24 , 25 ) . Therefore, study on role of leucine in porcine myoblast differentiation is absolutely essential.…”

mentioning

confidence: 99%

Leucine promotes differentiation of porcine myoblasts through the protein kinase B (Akt)/Forkhead box O1 signalling pathway

et al. 2018

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Leucine, one of the branched-chain amino acids, is the only amino acid to regulate protein turnover in skeletal muscle. Leucine not only increases muscle protein synthesis, but also decreases muscle protein degradation. It is well documented that leucine plays a positive role in differentiation of murine muscle cells. However, the role of leucine on porcine myoblast differentiation and its mechanism remains unclear. In this study, porcine myoblasts were induced to differentiate with differentiation medium containing different concentrations of leucine, and wortmannin was used to interdict the activity of protein kinase B (Akt). We found that leucine increased the number of myosin heavy chain-positive cells and creatine kinase activity. Moreover, leucine increased the mRNA and protein levels of myogenin and myogenic determining factor (MyoD). In addition, leucine increased the levels of phosphorylated Akt/Akt and phosphorylated Forkhead box O1 (P-FoxO1)/FoxO1, as well as decreased the protein level of FoxO1. However, wortmannin, a specific repressor of PI3K/Akt signalling pathway, attenuated the positive role of leucine on porcine myoblast differentiation. Our results suggest that leucine promotes porcine myoblast differentiation through the Akt/FoxO1 signalling pathway.

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“…In PSCs in skeletal muscle miR-22 inhibits the proliferation through targeting the cyclin D1, cyclin B1, and p21 but promotes the differentiation through regulating the expression of MyoG and MyHC [61]. Further, miR-27a inhibits the expression of MyoG and it also regulates the protein kinase B/forkhead box protein O1 (Akt/FoxO1) signal pathway during porcine myogenesis [75] while miR27b acts to promote PSCs myogenesis by targeting MyoD family inhibitor (MDFI) [76]. Besides, mi-34c, inhibits PSCs proliferation and promotes PSCs differentiation.…”

Section: Role Of Mirnas In Myogenesismentioning

confidence: 99%

Role of microRNAs in myogenesis and their effects on meat quality in pig — A review

Iqbal

Ping

Ali

et al. 2020

Asian-Australas J Anim Sci

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The demand for food is increasing day by day because of the increasing global population. Therefore, meat, the easiest and largely available source of protein, needs to be produced in large amounts with good quality. The pork industry is a significant shareholder in fulfilling the global meat demands. Notably, myogenesis- development of muscles during embryogenesis- is a complex mechanism which culminates in meat production. But the molecular mechanisms which govern the myogenesis are less known. The involvement of miRNAs in myogenesis and meat quality, which depends on factors such as myofiber composition and intramuscular fat contents which determine the meat color, flavor, juiciness, and water holding capacity, are being extrapolated to increase both the quantity and quality of pork. Various kinds of microRNAs (miRNAs), miR-1, miR-21, miR22, miR-27, miR-34, miR-127, miR-133, miR-143, miR-155, miR-199, miR-206, miR-208, miR-378, and miR-432 play important roles in pig skeletal muscle development. Further, the quality of meat also depends upon myofiber which is developed through the expression of different kinds of miRNAs at different stages. This review will focus on the mechanism of myogenesis, the role of miRNAs in myogenesis, and meat quality with a focus on the pig.

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Effects of MicroRNA-27a on Myogenin Expression and Akt/FoxO1 Signal Pathway during Porcine Myoblast Differentiation

Cited by 9 publications

References 42 publications

Propofol Alleviates DNA Damage Induced by Oxygen Glucose Deprivation and Reperfusion via FoxO1 Nuclear Translocation in H9c2 Cells

Propofol Alleviates DNA Damage Induced by Oxygen Glucose Deprivation and Reperfusion via FoxO1 Nuclear Translocation in H9c2 Cells

Leucine promotes differentiation of porcine myoblasts through the protein kinase B (Akt)/Forkhead box O1 signalling pathway

Role of microRNAs in myogenesis and their effects on meat quality in pig — A review

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