“…The tocopherols were monitored with a fluorescence spectrophotometer (Shimadzu RF-535, Shimadzu Instruments Inc, Kyoto, Japan) set at excitation wavelength 295 nm and emission wavelength 320 nm, quantitated by comparison with peak areas of internal standard (2,3,5,7,8-pentamethyl-6-hydroxy chroman, Eisai Co, Tokyo, Japan). The other HPLC conditions were as reported previously (Yoshida and Kajimoto 1989).…”