Effects of midazolam on equine innate immune response: a flow cytometric study

Massoco, Cristina de Oliveira; Palermo-Neto, João

doi:10.1016/s0165-2427(03)00097-7

Cited by 65 publications

(48 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Next, it was centrifuged, washed twice with cold PBS, and resuspended with PBS (10 g PI-bacteria/100 l PBS). A separate aliquot was not treated with the serum for nonopsonized phagocytosis (19,38,39).…”

Section: Nonspecific Immunostimulator Barodonmentioning

confidence: 99%

Immunostimulatory Effects of the Anionic Alkali Mineral Complex BARODON on Equine Lymphocytes

Koo

Ryu

Ahn

et al. 2006

Clin Vaccine Immunol

View full text Add to dashboard Cite

Previous studies have shown that the anionic alkali mineral complex BARODON has an immunoenhancing effect on pigs as an adjuvant and as a nonspecific immunostimulant. Likewise, the equine immune system has been defined with various monoclonal antibodies specific to equine leukocyte differentiation antigens to determine the possibility of enhancing equine resistance to respiratory diseases and promoting other immunostimulatory effects with the application of BARODON. Compared with the control group, after 3 weeks of treatment, BARODON-treated groups showed higher proportions of cells (P < 0.05) expressing major histocompatibility complex class II and CD2, CD4؉ , CD4 ؉ CD25 ؉ , CD8 ؉ , and CD8 ؉ CD25 ؉ T lymphocytes, dendritic cells, and surface immunoglobulin M ؉ B lymphocytes in peripheral blood, as well as enhanced cell proliferative responses with phytohemagglutinin and increased phagocytic activity against Streptococcus equi and Staphylococcus aureus strains with high antibiotic resistance, the bacteria frequently identified as etiologic agents of equine respiratory diseases at the Seoul Race Park in Seoul, Korea. This study shows that BAR-ODON may act as an immunostimulator and can be an effective alternative to antimicrobial feed additives for nonspecific improvements in equine immune responses, particularly against respiratory diseases.Equine respiratory disease is believed to be the second most important cause of poor performance, interruption of training, and premature retirement among performance horses (41,50,61,62,72). In Seoul, Korea, the Seoul Race Park experienced yearly epizootics of infectious upper respiratory diseases (IURD), with an estimated incidence of 29.6%, from 2001 to 2005. IURD affects the nasal passages and throat region and results in chronic coughing, exercise intolerance, weight loss, and nasal discharge

show abstract

Section: Nonspecific Immunostimulator Barodonmentioning

confidence: 99%

Immunostimulatory Effects of the Anionic Alkali Mineral Complex BARODON on Equine Lymphocytes

Koo

Ryu

Ahn

et al. 2006

Clin Vaccine Immunol

View full text Add to dashboard Cite

show abstract

“…For instance, a variety of stressors have been found to alter immune responses in experimental animals [5,6] . These effects, however, depend on the type of stressor, its duration and frequency, the temporal rela-tionship between stress and immunological stimulus and, among many other variables, the subjects' ability to control or escape from stressors [7][8][9] .…”

Section: Introductionmentioning

confidence: 99%

Cohabitation with a Sick Cage Mate: Effects on Ascitic Form of Ehrlich Tumor Growth and Macrophage Activity

Alves

Vismari

Palermo-Neto³

2007

Neuroimmunomodulation

View full text Add to dashboard Cite

The present study was designed to evaluate the effects of mice cohabitation with a sick conspecific cage mate on peritoneal macrophage activity and on resistance to Ehrlich tumor growth. Female mice housed in pairs were divided into control and experimental groups. One mouse of each control pair was inoculated with NaCl (0.1 ml/10 g) intraperitoneally and the other, called ‘companion of healthy partner’ (CHP), was kept undisturbed. One animal of each experimental pair of mice was inoculated with 5.0 × 10⁶ Ehrlich tumor cells intraperitoneally and the other, the subject of this study, was called ‘companion of sick partner’ (CSP). Peritoneal macrophages were removed from CSP and CHP mice to analyze resident macrophage activity (experiment 1), macrophage activity after Mycobacterium bovis (experiment 2) or Ehrlich tumor cells (experiment 3) in vivo inoculations. The resistance of CSP and CHP mice to Ehrlich tumor growth was also analyzed (experiment 4). Differences between groups were not found on resident macrophage activity. However, Onco-BCG- and Ehrlich tumor-activated macrophages from CSP mice presented a decreased intensity and percentage of phagocytosis and an increased respiratory burst in the presence of Staphylococcus aureus stimulation in vitro. CSP animals at the same time displayed a decreased resistance to Ehrlich tumor growth. These data were discussed in light of a possible psychological stress effect imposed by the housing condition on mice’s peritoneal macrophage activity and, as a consequence, on their resistance to Ehrlich tumor growth.

show abstract

“…A flow cytometer (FACSCalibur®, Becton Dickinson Immunocytometry Systems, San Jose, Calif., USA) interfaced with a Macintosh G4 computer was used to sort cells. L-selectin expression was determined by gating cell types according to their properties on forward scatter and side scatter plots, as described elsewhere [29]. Fluorescence data were collected on a log scale, and red fluorescence was measured at 585 ± 42 nm (FL2 detector); data were analyzed using FlowJo software.…”

Section: Methodsmentioning

confidence: 99%

3,4-Methylenedioxymethamphetamine (Ecstasy) Decreases Inflammation and Airway Reactivity in a Murine Model of Asthma

Stankevicius

Ferraz-de-Paula

Ribeiro

et al. 2012

Neuroimmunomodulation

View full text Add to dashboard Cite

Objective: 3,4-Methylenedioxymethamphetamine (MDMA), or ecstasy, is a synthetic drug used recreationally, mainly by young people. It has been suggested that MDMA has a Th cell skewing effect, in which Th1 cell activity is suppressed and Th2 cell activity is increased. Experimental allergic airway inflammation in ovalbumin (OVA)-sensitized rodents is a useful model to study Th2 response; therefore, based on the Th2 skewing effect of MDMA, we studied MDMA in a model of allergic lung inflammation in OVA-sensitized mice. Methods: We evaluated cell trafficking in the bronchoalveolar lavage fluid, blood and bone marrow; cytokine production; L-selectin expression and lung histology. We also investigated the effects of MDMA on tracheal reactivity in vitro and mast cell degranulation. Results: We found that MDMA given prior to OVA challenge in OVA-sensitized mice decreased leukocyte migration into the lung, as revealed by a lower cell count in the bronchoalveolar lavage fluid and lung histologic analysis. We also showed that MDMA decreased expression of both Th2-like cytokines (IL-4, IL-5 and IL-10) and adhesion molecules (L-selectin). Moreover, we showed that the hypothalamus-pituitary-adrenal axis is partially involved in the MDMA-induced reduction in leukocyte migration into the lung. Finally, we showed that MDMA decreased tracheal reactivity to methacholine as well as mast cell degranulation in situ. Conclusions: Thus, we report here that MDMA given prior to OVA challenge in OVA-sensitized allergic mice is able to decrease lung inflammation and airway reactivity and that hypothalamus-pituitary-adrenal axis activation is partially involved. Together, the data strongly suggest an involvement of a neuroimmune mechanism in the effects of MDMA on lung inflammatory response and cell recruitment to the lungs of allergic animals.

show abstract

Effects of midazolam on equine innate immune response: a flow cytometric study

Cited by 65 publications

References 38 publications

Immunostimulatory Effects of the Anionic Alkali Mineral Complex BARODON on Equine Lymphocytes

Immunostimulatory Effects of the Anionic Alkali Mineral Complex BARODON on Equine Lymphocytes

Cohabitation with a Sick Cage Mate: Effects on Ascitic Form of Ehrlich Tumor Growth and Macrophage Activity

3,4-Methylenedioxymethamphetamine (Ecstasy) Decreases Inflammation and Airway Reactivity in a Murine Model of Asthma

Contact Info

Product

Resources

About