Zygomycetes especially oleaginous fungi have a great ability to produce huge quantities of lipids high in economically important poly-unsaturated fatty acids. About 32 isolates were isolated from oleaginous zygotes. Six of the 32 were identified as Rhizopus spp. identified. Tested for production of high levels of lipids. 6 out of 32 have the ability to produce huge quantities of lipids. The Rhizopus sp. UC 13 strain was selected for further optimization as it produced 1.05 gl -1 lipid with a lipid content of 39.21%. The production of lipids was increased using the Taguchi method which selected a number of factors at varying levels for the optimization process. Glucose, ammonium sulfate, temperature, pH and time directly affect lipogenesis. Saturated fatty acids (SFAs) were more prevalent than unsaturated fatty acids (USFAs) based on the GC-Mass profile, which also revealed that