Effects of miRNA-197 overexpression on proliferation, apoptosis and migration in levonorgestrel treated uterine leiomyoma cells

Wu, Xiaoli; Jing, Ling; Fu, Ziyi; Ji, Chenbo; Wu, Jiangping; Xu, Qing

doi:10.1016/j.biopha.2015.02.004

Cited by 15 publications

(10 citation statements)

References 44 publications

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“…In addition to qPCR, which suggested an upregulation of miR-15b levels, in situ hybridization was performed to detect its localization in UL tissues. These results were consistent with those of previous studies [ 18 , 31 , 32 , 33 ]. Several investigators reported that the marked upregulation in miR-15b expression levels in UL cells might modulate numerous cellular biological processes such as cell proliferation, division, apoptosis, migration, invasion, metabolism, stress, angiogenesis, and drug resistance [ 34 , 35 , 36 ].…”

Section: Discussionsupporting

confidence: 94%

Variation in MicroRNA Expression Profile of Uterine Leiomyoma with Endometrial Cavity Distortion and Endometrial Cavity Non-Distortion

Kim

Shin

et al. 2018

IJMS

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The expression profile of microRNA (miRNA) in uterine leiomyoma (UL) cells is different from that in normal uterine myometrial (UM) cells. The effect of UL cells on uterine receptivity might vary according to their ability to distort the uterine endometrial cavity. However, the variation in miRNA expression profiles between endometrial cavity-distorting leiomyoma (ECDL) and endometrial cavity non-distorting leiomyoma (ECNDL) cells remains unknown. This study aimed to elucidate whether the expression profile of miRNAs in ECDL cells is dissimilar to that of ECNDL cells in uterus. Pelviscopic myomectomy was performed to obtain tissue samples of UL and their corresponding normal UM tissues (matched) from patients with UL (n = 26), among whom women with ECNDL and ECDL numbered 15 and 11, respectively. The relative expression of hsa-miR-15b, -29a, -29b, -29c, -197, and -200c as well as the candidate target genes in UL cells was compared to those in the matched UM cells using qRT-PCR to assess their ability to cause ECD. The spatial expression of miRNAs and target genes in the UL tissues was analyzed using in situ hybridization. Target gene expression was analyzed using qPCR after transfection with the mimics and inhibitors of miRNAs in UL cells. The relative expression level of miR-15b was upregulated, and the relative expression levels of miR-29a, -29b, -29c, -197, and -200c were downregulated in UL cells compared to those in UM cells. The relative expression levels of progesterone receptor, estrogen receptor, and matrix metalloproteinases (MMPs) were upregulated in UL cells compared to those in UM cells. The relative expression levels of miR-29c and -200c were downregulated, and the relative expression levels of estrogen receptor, MMPs and tissue inhibitors of metalloproteinases (TIMPs) were upregulated in ECDL cells compared to those in ECNDL cells. The expression profile of miRNAs in UL cells varied with respect to the occurrence or absence of endometrial cavity distortion. The biochemical properties of UL might be regulated by miRNAs in order to alter their effect on structural homeostasis of the uterus.

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Section: Discussionsupporting

confidence: 94%

Variation in MicroRNA Expression Profile of Uterine Leiomyoma with Endometrial Cavity Distortion and Endometrial Cavity Non-Distortion

Kim

Shin

et al. 2018

IJMS

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“…Another study has reported miR‐520c and miR‐373 as prometastatic, which enhanced the expression of matrix metalloproteinase by targeting sirtuin‐1 and mammalian target of rapamycin in fibrosarcoma . Moreover, among the plethora of miRNAs studied so far in cancers, miR‐197 has been seen to play differential roles, either as a tumor suppressor or oncogene in several tumorigenic pathways, such as cell proliferation, differentiation, apoptosis, angiogenesis, invasion, and metastasis, drug resistance, etc . However, its roles in oncogenesis of fibrosarcoma are not yet explored.…”

Section: Introductionmentioning

confidence: 99%

miR‐197‐5p inhibits sarcomagenesis and induces cellular senescence via repression of KIAA0101

Jain

Roy

Das

et al. 2019

Molecular Carcinogenesis

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The abnormal expressions of microRNAs (miRNAs) are known to be associated with various pathophysiological processes that lead to the development of a plethora of diseases including cancer. Among several miRNAs studied so far, miR-197 has been reported to play a vital role either as an oncogene or tumor suppressor in different cancers. However, its role in carcinogenesis of fibrosarcoma has not yet been elucidated. Therefore, the current study investigated the role of miR-197-5p, which is significantly downregulated in HT1080 fibrosarcoma cells compared to IMR90-tert fibroblast cells. The transient overexpression of miR-197-5p causes a significant decrease in viability and proliferation of fibrosarcoma cells in both concentration-and time-dependent manners. Interestingly, we did not observe any significant changes in cell cycle pattern or apoptotic cell populations, but rather noticed cellular senescence of fibrosarcoma cells upon overexpression of miR-197-5p. Further, this miRNA suppresses the metastatic properties, such as migration, invasion, and anchorageindependent growth of fibrosarcoma possibly through targeting KIAA0101, which is a proliferating cell nuclear antigen-associated factor and overexpressed in the malignancy. In nutshell, our result revealed that miR-197-5p acts as an oncosuppressor miRNA in fibrosarcoma through target regulation of KIAA0101, which can be exploited for developing RNA-based therapeutic strategies for the cure of this malignancy.

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“…Another interesting finding of this study was an observation that levonorgestrel could induce miR-197 expression in UF tissue. This overexpression was suggested to play an important role in cell proliferation and apoptosis modulation [98]. According to the in vitro study of Ling et al (2015) [99] the downregulation of miR-197 increased cell growth rate and induced cell cycle arrest in the G0/G1 phase.…”

Section: The Mir-197 Familymentioning

confidence: 98%

“…Wu et al (2015) [98] were the first to confirm that miR-197 was downregulated in human UFs. In the same study miR-197 was found to inhibit cell proliferation, induce apoptosis and block cells migration in vitro [98]. Another interesting finding of this study was an observation that levonorgestrel could induce miR-197 expression in UF tissue.…”

Section: The Mir-197 Familymentioning

confidence: 99%

“…According to currently available data miR-197 has a confirmed role in human malignancies where it targets key tumorigenic and suppressive genes [97]. Wu et al (2015) [98] were the first to confirm that miR-197 was downregulated in human UFs. In the same study miR-197 was found to inhibit cell proliferation, induce apoptosis and block cells migration in vitro [98].…”

Section: The Mir-197 Familymentioning

confidence: 99%

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The Role of miRNA and Related Pathways in Pathophysiology of Uterine Fibroids—From Bench to Bedside

Ciebiera

Włodarczyk

Zgliczyński

et al. 2020

IJMS

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Uterine fibroids (UFs) are the most common benign tumors of the female genital tract. Their prevalence usually is estimated at 30–40%, but may reach up to 70–80% in predisposed groups of women. UFs may cause various clinical issues which might constitute the major reason of the overall deterioration of the quality of life. The mechanisms leading to UFs formation and growth still remain poorly understood. The transformation of smooth muscle cells of the uterus into abnormal, immortal cells, capable of clonal division, is thought to be a starting point of all pathways leading to UF formation. Micro-ribonucleic acids (miRNAs) are non-coding single-stranded RNAs about 22 nucleotides in length, that regulate gene expression. One of recent advances in this field is the comprehension of the role of miRNAs in tumorigenesis. Alterations in the levels of miRNAs are related to the formation and growth of several tumors which show a distinct miRNA signature. The aim of this review is to summarize the current data about the role of miRNAs in the pathophysiology of UFs. We also discuss future directions in the miRNA research area with an emphasis on novel diagnostic opportunities or patient-tailored therapies. In our opinion data concerning the regulation of miRNA and its gene targets in the UFs are still insufficient in comparison with gynecological malignancies. The potential translational use of miRNA and derived technologies in the clinical care is at the early phase and needs far more evidence. However, it is one of the main areas of interest for the future as the use of miRNAs in the diagnostics and treatment of UFs is a new and exciting opportunity.

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Effects of miRNA-197 overexpression on proliferation, apoptosis and migration in levonorgestrel treated uterine leiomyoma cells

Cited by 15 publications

References 44 publications

Variation in MicroRNA Expression Profile of Uterine Leiomyoma with Endometrial Cavity Distortion and Endometrial Cavity Non-Distortion

Variation in MicroRNA Expression Profile of Uterine Leiomyoma with Endometrial Cavity Distortion and Endometrial Cavity Non-Distortion

miR‐197‐5p inhibits sarcomagenesis and induces cellular senescence via repression of KIAA0101

The Role of miRNA and Related Pathways in Pathophysiology of Uterine Fibroids—From Bench to Bedside

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