Effects of Mitochondrial DNA Deletion and OSW-1 on PI3K-AKT Signaling Pathway PCR Array in SK-Hep1 Cells

Abstract

To investigate the effect of mitochondrial DNA deletion and OSW-1 on PI3K-AKT signaling pathway PCR Array in SK-Hep1 hepatocellular carcinoma cells, we prepared SK-Hep1 cells with mtDNA deletion, that is, p 0 SK Hep. Then the OSW-1 of 100 ng/L was used to intervene SK-Hep1 and p 0 SK-Hep1. RT-qPCR was used to detect the difference of gene expression on PI3K-AKT signaling pathway PCR Array in four groups of cells. The gene expression of TLR4, FOS and TSC2 markers in SK-Hep1 cells treated with OSW-1 were significantly increased. The gene expressions of PDPK1, GJA1, TLR4 and TSC2 markers were significantly increased in p 0 SK-Hep1 cells, and the gene expressions of IRAK1 and GJA1 markers were significantly increased in p 0 SK-Hep1 cells treated with OSW-1. OSW-1 mainly affects the genes related to TLR4 pathway on PI3K-AKT signaling pathway PCR Array in SK-Hep1 HCC cells. P 0 SK-Hep1 mainly affects the upstream PDK1 gene and downstream TSC2 gene of Akt on PI3K-AKT signaling pathway PCR Array, and also affects the gene expression of gap junction at the same time.

Chemical Probes and Activity-Based Protein Profiling for Cancer Research

“…In parallel, OSW-1 is a potent anticancer saponin that has a positive effect on breast cancer cells [ 59 ]. Moreover, OSW-1 is known to activate PI3K-AKT signaling in hepatocellular carcinoma cells (e.g., SK-Hep1) [ 60 ]. A photoactive diaziridine group was incorporated into OSW-1 to produce an effective anticancer agent with photophysical properties [ 61 ].…”