An energy-based low-cycle fatigue model was proposed for applications at a range of temperatures. An existing model was extended to the integrated approach, incorporating the simultaneous effects of strain rate and temperature. A favored material at high temperature, type 316L stainless steel, was selected in this study and its material characteristics were investigated. Tensile tests and low-cycle fatigue tests were performed using several strain rates at a temperature ranging from room temperature to 650 °C. Material properties were obtained in terms of temperature using the displacement-controlled tensile tests and further material response were investigated using strain-controlled tensile tests. Consequently, no pronounced reduction in strengths occurred at temperatures between 300 and 550 °C, and a negative strain rate response was observed in the temperature range. Based on the low-cycle fatigue tests by varying strain rates and temperature, it was found that a normalized plastic strain energy density and a strain-rate modified cycle were successfully correlated. The accuracy of the model was discussed by comparing between predicted and experimental lives.