Effects of MK801 on Fos expression in the rat brainstem after unilateral labyrinthectomy

Kitahara, Tadashi; Takeda, Noriaki; Saika, Takanori; Kubo, Takeshi; Kiyama, Hiroshi

doi:10.1016/0006-8993(95)00950-u

Cited by 69 publications

(37 citation statements)

References 35 publications

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“…Other symptoms of UL, such as rolling and circling behavior, torsion of the head, and limb extension, also disappeared within 1 hr to 3 d after UL, depending on the symptoms. These observations were consistent with previous reports that described SN frequency and other behavioral symptoms after UL in rats (Kitahara et al, 1995;Cirelli et al, 1996;Goto et al, 1997). These results indicate that the early stage of vestibular compensation develops by ϳ3 d after UL in rats.…”

Section: Vestibular Compensation After Unilateral Labyrinthectomysupporting

confidence: 83%

“…In particular, the mapping of immediateearly gene expression has consistently demonstrated the involvement of three specific subregions in the brainstem: the MVN (Kaufman et al, 1992;Kitahara et al, 1995;Darlington et al, 1996), the PrH (Kaufman et al, 1992;Kitahara et al, 1995;Cirelli et al, 1996), and the medial region of the IO (Kaufman et al, 1992;Kitahara et al, 1995;Cirelli et al, 1996;Sato et al, 1997). On the basis of this neurochemical evidence, we further investigated the molecular mechanisms that underlie vestibular compensation by focusing on changes in BDNF mRNA expression levels in these brainstem regions after UL.…”

Section: Evaluation Of Mrna Expression Levels By Quantitative Rt-pcrmentioning

confidence: 99%

“…Several studies have demonstrated induction of c-fos mRNA and c-Fos protein after UL in the MVN, PrH, and IO by in situ hybridization and immunohistochemical staining (Kaufman et al, 1992;Kitahara et al, 1995;Cirelli et al, 1996;Sato et al, 1997). We next quantitatively evaluated the temporal dynamics of c-fos mRNA expression (Fig.…”

Section: C-fos Mrna Induction After Unilateral Labyrinthectomymentioning

confidence: 99%

“…All of the animals rapidly recovered from this anesthesia no later than 5 min after the surgery and immediately showed behavioral signs of UL. To characterize the compensatory processes after UL, we first investigated the time course of disappearance of horizontal SN after UL as an index of development of vestibular compensation (Kitahara et al, 1995;Goto et al, 1997). We monitored eye movements of rats by an infrared CCD camera in darkness (Fig.…”

Section: Vestibular Compensation After Unilateral Labyrinthectomymentioning

confidence: 99%

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Spatiotemporal Dynamics of Brain-Derived Neurotrophic Factor mRNA Induction in the Vestibulo-Olivary Network during Vestibular Compensation

Li¹,

Hashimoto²,

Tokuyama

et al. 2001

J. Neurosci.

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Vestibular compensation, which is the behavioral recovery from vestibular dysfunction produced by unilateral labyrinthectomy (UL), is attributed to functional and structural reorganization of neural networks in the central vestibular system. To assess the possible contribution of brain-derived neurotrophic factor (BDNF) to this recovery process, we investigated changes in mRNA expression levels in the central vestibular system after UL. We evaluated BDNF mRNA expression levels by quantitative reverse transcription-PCR and in situ hybridization. We found that BDNF mRNA is differentially induced in the medial vestibular nucleus ipsilateral to UL and in the prepositus hypoglossi and inferior olive on the contralateral side. The BDNF mRNA induction lasted for at least 24 hr and returned to the basal expression level within 72 hr after UL. In contrast to BDNF mRNA induction, the expression of an immediate-early gene, c-fos, quickly reached the maximum level at 3 hr and decreased to the basal level within 24 hr after UL. Neither BDNF or c-fos induction was observed in sham-operated animals. The persistent induction of BDNF after UL temporally corresponded to early behavioral manifestations of vestibular compensation. We further found that trkB mRNA was expressed in the central vestibular network at high levels, although its expression levels did not change over time after UL. Because BDNF is implicated in regulating synaptic structure and function, these results provide support for the hypothesis that BDNF is involved in neuronal reorganization that allows vestibular compensation.

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Section: Vestibular Compensation After Unilateral Labyrinthectomysupporting

confidence: 83%

Section: Evaluation Of Mrna Expression Levels By Quantitative Rt-pcrmentioning

confidence: 99%

Section: C-fos Mrna Induction After Unilateral Labyrinthectomymentioning

confidence: 99%

Section: Vestibular Compensation After Unilateral Labyrinthectomymentioning

confidence: 99%

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Spatiotemporal Dynamics of Brain-Derived Neurotrophic Factor mRNA Induction in the Vestibulo-Olivary Network during Vestibular Compensation

Li¹,

Hashimoto²,

Tokuyama

et al. 2001

J. Neurosci.

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“…Neural plasticity is one key process in potential recovery after stroke (8). Most surgical techniques reported until now include extensive extirpation of the wall of the meatus acusticus externus and structures of middle ear (1,4,7,9,12,15,16,22,25). The specific anatomy of the rodent skull, however, provides an alternative surgical approach through the tympanic bulla (6,10,11,13).…”

Section: Introductionmentioning

confidence: 99%

A simple standard technique for labyrinthectomy in the rat: A methodical communication with a detailed description of the surgical process

Nádasy

Raffai

Fehér

et al. 2016

Physiology International

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Aims: Labyrinthectomized rats are suitable models to test consequences of vestibular lesion and are widely used to study neural plasticity. We describe a combined microsurgical-chemical technique that can be routinely performed with minimum damage. Methods: Caudal leaflet of the parotis is elevated. The tendinous fascia covering the bulla is opened frontally from the sternomastoid muscle's tendon while sparing facial nerve branches. A 4 mm diameter hole is drilled into the bulla's hind lower lateral wall to open the common (in rodents) mastoid-tympanic cavity. The cochlear crista (promontory) at the lower posterior part of its medial wall is identified as a bony prominence. A 1 mm diameter hole is drilled into its lower part. The perilymphatic/endolymphatic fluids with tissue debris of the Corti organ are suctioned. Ethanol is injected into the hole. Finally, 10 μL of sodium arsenite solution (50 μM/mL) is pumped into the labyrinth and left in place for 15 min. Simple closure in two layers (fascia and skin) is sufficient. Results and conclusion: All rats had neurological symptoms specific for labyrinthectomy (muscle tone, body position, rotatory movements, nystagmus, central deafness). Otherwise, their behavior was unaffected, drinking and eating normally. After a few days, they learned to balance relying on visual and somatic stimuli (neuroplasticity).

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Fos‐enkephalin signaling in the rat medial vestibular nucleus facilitates vestibular compensation

Kitahara

Kaneko

Horii

et al. 2006

J of Neuroscience Research

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In the present study, we first observed up-regulation in preproenkephalin (PPE)-like immunoreactivity (-LIR), a precursor of Met- and Leu-enkephalin, in the rat ipsilateral medial vestibular nucleus (ipsi-MVN) after unilateral labyrinthectomy (UL). By means of double-staining immunohistochemistry with PPE and Fos, a putative regulator of PPE gene expression, we revealed that some of these PPE-LIR neurons were also Fos immunopositive. The time course of decay of these double-stained neurons was quite parallel to that of UL-induced behavioral deficits. This suggests that these double-labeled neurons could have something to do with development of vestibular compensation. We next examined correlation between Fos and PPE expression in the ipsi-MVN by means of a 15-min pre-UL application of antisense oligonucleotide probes against c-fos mRNA into the ipsi-MVN. Gel shift assay and Western blotting revealed that elimination of Fos expression significantly reduced both AP-1 DNA binding activity and PPE expression in the ipsi-MVN after UL. C-fos antisense study also revealed that depression of Fos-PPE signaling in the ipsi-MVN caused significantly more severe behavioral deficits during vestibular compensation. Furthermore, studies with PPE antisense and naloxone, an opioid receptor antagonist, demonstrated that specific depression of enkephalinergic effects in the ipsi-MVN significantly delayed vestibular compensation. All these findings suggest that, immediately after UL, Fos induced in some of the ipsi-MVN neurons could regulate consequent PPE expression via the AP-1 activation and facilitate the restoration of balance between bilateral MVN activities via the opioid receptor activation, resulting in progress of vestibular compensation.

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Effects of MK801 on Fos expression in the rat brainstem after unilateral labyrinthectomy

Cited by 69 publications

References 35 publications

Spatiotemporal Dynamics of Brain-Derived Neurotrophic Factor mRNA Induction in the Vestibulo-Olivary Network during Vestibular Compensation

Spatiotemporal Dynamics of Brain-Derived Neurotrophic Factor mRNA Induction in the Vestibulo-Olivary Network during Vestibular Compensation

A simple standard technique for labyrinthectomy in the rat: A methodical communication with a detailed description of the surgical process

Fos‐enkephalin signaling in the rat medial vestibular nucleus facilitates vestibular compensation

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