Effects of Mn-doping on the structure and biological properties of β-tricalcium phosphate

Torres, Paula M.C.; Vieira, Sandra I.; Cerqueira, Ângela; Pina, Sandra; Silva, O.A.B. da Cruz; Abrantes, J.C.C.; Ferreira, J.M.F.

doi:10.1016/j.jinorgbio.2014.03.013

Cited by 87 publications

(43 citation statements)

References 44 publications

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“…On the other hand, the SF/MnTCP scaffolds presented higher ALP activity, even in the basal medium condition. It has been shown that Mn regulates bone growth and remodeling, presenting a high capacity to stimulate cell adhesion [Torres et al, 2014].…”

Section: Discussionmentioning

confidence: 99%

“…Finally, Alizarin red staining was carried out to qualitatively assess the induction of osteogenic differentiation of the hASCs by the ionic-doped scaffolds. [Pina et al, 2009[Pina et al, , 2010bTorres et al, 2014]. The molar ratio of (Ca + X)/P = 1.48 (where X corresponds to Sr, Zn, or Mn) was used in order to avoid hydroxyapatite (HAp) formation (with a pH of approx.…”

Section: +mentioning

confidence: 99%

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Biofunctional Ionic-Doped Calcium Phosphates: Silk Fibroin Composites for Bone Tissue Engineering Scaffolding

Pina

Canadas

Jiménez³

et al. 2017

Cells Tissues Organs

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The treatment and regeneration of bone defects caused by traumatism or diseases have not been completely addressed by current therapies. Lately, advanced tools and technologies have been successfully developed for bone tissue regeneration. Functional scaffolding materials such as biopolymers and bioresorbable fillers have gained particular attention, owing to their ability to promote cell adhesion, proliferation, and extracellular matrix production, which promote new bone growth. Here, we present novel biofunctional scaffolds for bone regeneration composed of silk fibroin (SF) and β-tricalcium phosphate (β-TCP) and incorporating Sr, Zn, and Mn, which were successfully developed using salt-leaching followed by a freeze-drying technique. The scaffolds presented a suitable pore size, porosity, and high interconnectivity, adequate for promoting cell attachment and proliferation. The degradation behavior and compressive mechanical strengths showed that SF/ionic-doped TCP scaffolds exhibit improved characteristics for bone tissue engineering when compared with SF scaffolds alone. The in vitro bioactivity assays using a simulated body fluid showed the growth of an apatite layer. Furthermore, in vitro assays using human adipose-derived stem cells presented different effects on cell proliferation/differentiation when varying the doping agents in the biofunctional scaffolds. The incorporation of Zn into the scaffolds led to improved proliferation, while the Sr- and Mn-doped scaffolds presented higher osteogenic potential as demonstrated by DNA quantification and alkaline phosphatase activity. The combination of Sr with Zn led to an influence on cell proliferation and osteogenesis when compared with single ions. Our results indicate that biofunctional ionic-doped composite scaffolds are good candidates for further in vivo studies on bone tissue regeneration.

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Section: Discussionmentioning

confidence: 99%

Section: +mentioning

confidence: 99%

Biofunctional Ionic-Doped Calcium Phosphates: Silk Fibroin Composites for Bone Tissue Engineering Scaffolding

Pina

Canadas

Jiménez³

et al. 2017

Cells Tissues Organs

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“…MC3T3 cells grown on coverslips-containing 35 mm petri dishes and subjected for 21 DIV to LF and HF stimulation were fixed in 4% paraformaldehyde for 20 min and permeabilized with 0.1% Triton X-100 in PBS (15 min/RT). Immunocytochemistry of collagen-I, F-actin staining with phalloidin, and nuclear staining with DAPI was as previously described 59 . The anti-collagen-I antibody (1:250 in 3% BSA-PBS), a FITC-conjugated secondary antibody (1:300 in 3% BSA-PBS), and red fluorescing Alexa 568-labelled Phalloidin (1:500) were used.…”

Section: Methodsmentioning

confidence: 99%

“…The anti-collagen-I antibody (1:250 in 3% BSA-PBS), a FITC-conjugated secondary antibody (1:300 in 3% BSA-PBS), and red fluorescing Alexa 568-labelled Phalloidin (1:500) were used. Images were acquired with a plan-Neofluor 40x/1.30 oil objective in a LSM 510 META confocal microscope (Zeiss, Jena, Germany) (as previously described 59 ), in the iBiMED’s Imaging Facility, a node of PPBI (Portuguese Platform of BioImaging).…”

Section: Methodsmentioning

confidence: 99%

New cosurface capacitive stimulators for the development of active osseointegrative implantable devices

Santos

Marote

Santos

et al. 2016

Sci Rep

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Non-drug strategies based on biophysical stimulation have been emphasized for the treatment and prevention of musculoskeletal conditions. However, to date, an effective stimulation system for intracorporeal therapies has not been proposed. This is particularly true for active intramedullary implants that aim to optimize osseointegration. The increasing demand for these implants, particularly for hip and knee replacements, has driven the design of innovative stimulation systems that are effective in bone-implant integration. In this paper, a new cosurface-based capacitive system concept is proposed for the design of implantable devices that deliver controllable and personalized electric field stimuli to target tissues. A prototype architecture of this system was constructed for in vitro tests, and its ability to deliver controllable stimuli was numerically analyzed. Successful results were obtained for osteoblastic proliferation and differentiation in the in vitro tests. This work provides, for the first time, a design of a stimulation system that can be embedded in active implantable devices for controllable bone-implant integration and regeneration. The proposed cosurface design holds potential for the implementation of novel and innovative personalized stimulatory therapies based on the delivery of electric fields to bone cells.

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“…Higher osteoblast-like cell-adhesion was observed on Mn-containing hydroxyapatites [27], compared to synthetic HA, and tricalcium phosphates containing this ion presented higher bone mineralisation rates [28]. An increase in the osteogenesis-related gene expression and collagen deposition rate was observed in Mn-containing titanium implants exposed to rat bone marrow mesenchymal stem cells [29]; an increased antibacterial activity was also observed in multifunctional materials containing this ion [29,30].…”

Section: Introductionmentioning

confidence: 99%

Osteogenic potential of sol–gel bioactive glasses containing manganese

Barrioni

Norris

et al. 2019

J Mater Sci: Mater Med

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We report that the release of manganese ions from bioactive glass provoked human mesenchymal stem cell (hMSC) differentiation down a bone pathway, whereas hMSCs exposed to the Mn-free glass did not differentiate. Bioactive glasses (BGs) are widely used for bone regeneration, and allow the incorporation of different ions with therapeutic properties into the glass network. Amongst the different ions with therapeutic benefits, manganese (Mn) has been shown to influence bone metabolism and activate human osteoblasts integrins, improving cell adhesion, proliferation and spreading. Mn has also been incorporated into bioceramics as a therapeutic ion for improved osteogenesis. Here, up to 4.4 mol% MnO was substituted for CaO in the 58S composition (60 mol% SiO2, 36 mol% CaO, 4 mol% P2O5) and its effects on the glass properties and capability to influence the osteogenic differentiation were evaluated. Mncontaining BGs with amorphous structure, high specific surface area and nanoporosity were obtained. The presence of Mn 2+ species was confirmed by X-ray photoelectron spectroscopy (XPS). Mn-containing BGs presented no cytotoxic effect on human mesenchymal stem cells (hMSCs) and enabled sustained ion release in culture medium. hMSCs osteogenic differentiation stimulation and influence on the mineralisation process was also confirmed through the alkaline phosphatase (ALP) activity, and expression of osteogenic differentiation markers, such as collagen type I, osteopontin and osteocalcin, which presented higher expression in the presence of Mn-containing samples compared to control. Mn incorporation offers great promise for obtaining glasses with superior properties for bone tissue regeneration.

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Effects of Mn-doping on the structure and biological properties of β-tricalcium phosphate

Cited by 87 publications

References 44 publications

Biofunctional Ionic-Doped Calcium Phosphates: Silk Fibroin Composites for Bone Tissue Engineering Scaffolding

Biofunctional Ionic-Doped Calcium Phosphates: Silk Fibroin Composites for Bone Tissue Engineering Scaffolding

New cosurface capacitive stimulators for the development of active osseointegrative implantable devices

Osteogenic potential of sol–gel bioactive glasses containing manganese

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