Effects of modulation of potassium channels in tobacco mosaic virus elimination

Sabella, Erika; Pierro, Roberto; Panattoni, Alessandra; Materazzi, Alberto; Vergine, Marzia; Bellis, Luigi De; Luvisi, Andrea

doi:10.1016/j.pmpp.2018.03.003

Cited by 4 publications

(2 citation statements)

References 36 publications

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“…In summary, we have determined the molecular arrangement of the Gc fusion domain and Gn within the floor domain of the BUNV spike, and additionally resolved drastic structural changes in the GP architecture in response to pH 6.3/K + , identifying an intermediate fusion state capable of interacting with target membranes. The ability of pharmacological K + channel inhibitors to disrupt infection by a number of bunyaviruses from different families (11,13,14,44), alongside the structural changes elicited by K + on both BUNV and HAZV, suggests a shared mechanism that could be exploited therapeutically.…”

Section: Discussionmentioning

confidence: 99%

Organisation of the orthobunyavirus tripodal spike and the structural changes induced by low pH and K⁺ during entry

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Charlton

Hellert

et al. 2022

Preprint

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Following internalisation, viruses employ the changing environment of maturing endosomes as cues to promote endosomal escape, a process mediated by viral glycoproteins. Specifically, we previously showed that both high [K+] and low pH promote entry of Bunyamwera virus (BUNV), the prototypical bunyavirus. Here, we used sub-tomogram averaging combined with AlphaFold, to generate a pseudo-atomic model of the whole glycoprotein envelope of BUNV. This allowed us to unambiguously locate the Gc fusion domain and its chaperone Gn within the floor domain of the spike. We also confirmed that low pH and high [K+] alters the viral glycoproteins, resulting in an activated intermediate state functionally-distinct from the highly ordered ground state, and we localize these changes to the floor domain. Biochemical data suggests that in this intermediate state the viral fusion loops are partially exposed and selectively interact with host cell membranes. Taken together, we reveal new mechanistic understanding of the requirements for virus entry.

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Section: Discussionmentioning

confidence: 99%

Organisation of the orthobunyavirus tripodal spike and the structural changes induced by low pH and K⁺ during entry

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Charlton

Hellert

et al. 2022

Preprint

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“…From its first detection at the end of 2013 in the Salento peninsula (Apulia region, southern Italy), Xylella fastidiosa represents an emerging threat in Europe, associated with a previously unknown disease on olive trees ( Olea europaea L.) which causes leaf scorch, a rapid decline and the death of trees (the so-called “olive quick decline syndrome”, OQDS) [ 8 ]. The symptoms are particularly severe on large old plants of the most susceptible cultivars Cellina di Nardò and Ogliarola [ 9 ], the most widespread in the whole area. Moreover, the further unexpected detections in France and Spain allow it as an expanding global pathogen [ 10 ], which could reshape not only agriculture but the landscape and human relations with plants [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization of the olive endophytic community in genotypes displaying a contrasting response to Xylella fastidiosa

Vergine,

Vita,

Casati

et al. 2024

BMC Plant Biol

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Background Endophytes mediate the interactions between plants and other microorganisms, and the functional aspects of interactions between endophytes and their host that support plant-growth promotion and tolerance to stresses signify the ecological relevance of the endosphere microbiome. In this work, we studied the bacterial and fungal endophytic communities of olive tree (Olea europaea L.) asymptomatic or low symptomatic genotypes sampled in groves heavily compromised by Xylella fastidiosa subsp. pauca, aiming to characterize microbiota in genotypes displaying differential response to the pathogen. Results The relationships between bacterial and fungal genera were analyzed both separately and together, in order to investigate the intricate correlations between the identified Operational Taxonomic Units (OTUs). Results suggested a dominant role of the fungal endophytic community compared to the bacterial one, and highlighted specific microbial taxa only associated with asymptomatic or low symptomatic genotypes. In addition, they indicated the occurrence of well-adapted genetic resources surviving after years of pathogen pressure in association with microorganisms such as Burkholderia, Quambalaria, Phaffia and Rhodotorula. Conclusions This is the first study to overview endophytic communities associated with several putatively resistant olive genotypes in areas under high X. fastidiosa inoculum pressure. Identifying these negatively correlated genera can offer valuable insights into the potential antagonistic microbial resources and their possible development as biocontrol agents.

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Regulatory effects of potassium channel blockers on potassium channel genes upon nervous necrosis virus infection in sevenband grouper Hyporthodus septumfasciatus

Rajendran,

Krishnan,

Kim

et al. 2024

Gene

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Effects of modulation of potassium channels in tobacco mosaic virus elimination

Cited by 4 publications

References 36 publications

Organisation of the orthobunyavirus tripodal spike and the structural changes induced by low pH and K⁺ during entry

Organisation of the orthobunyavirus tripodal spike and the structural changes induced by low pH and K⁺ during entry

Characterization of the olive endophytic community in genotypes displaying a contrasting response to Xylella fastidiosa

Regulatory effects of potassium channel blockers on potassium channel genes upon nervous necrosis virus infection in sevenband grouper Hyporthodus septumfasciatus

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Effects of modulation of potassium channels in tobacco mosaic virus elimination

Cited by 4 publications

References 36 publications

Organisation of the orthobunyavirus tripodal spike and the structural changes induced by low pH and K+ during entry

Organisation of the orthobunyavirus tripodal spike and the structural changes induced by low pH and K+ during entry

Characterization of the olive endophytic community in genotypes displaying a contrasting response to Xylella fastidiosa

Regulatory effects of potassium channel blockers on potassium channel genes upon nervous necrosis virus infection in sevenband grouper Hyporthodus septumfasciatus

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Organisation of the orthobunyavirus tripodal spike and the structural changes induced by low pH and K⁺ during entry

Organisation of the orthobunyavirus tripodal spike and the structural changes induced by low pH and K⁺ during entry