Effects of Moist Exposed Burn Therapy and Ointment (MEBT/MEBO) on the autophagy mTOR signalling pathway in diabetic ulcer wounds

Abstract

Context: Burn therapy (MEBT)/moist exposed burn ointment (MEBO) is an effective traditional Chinese medicine method to treat diabetic wound, but the mechanism is unclear. Autophagy has been proved to be closely related with wound healing, so MEBO/MEBT is hypothesized to promote diabetic wound healing by regulating autophagy. Objective: To explore the mechanism of moist exposed MEBT/MEBO promoting diabetic wound repair. Materials and methods: Eighty male Wistar rats were randomly assigned to control (n ¼ 20) and diabetic group induced by intraperitoneal injection of STZ (n ¼ 60), which were further randomly assigned to MEBO, Kangfuxin and model groups (n ¼ 20 each). All rats underwent full-thickness skin resection in the back. Wound healing was dynamically observed and wound tissues were collected at five time points for pathological examination, autophagosome and the expression of PI3K, Akt and mTOR. Results: The healing time in the control group was the shortest, no statistically significant difference was found between the MEBO and the Kangfuxin group (p ¼ 0.76). The morphology of autophagosomes ranged large to small, which was the most obvious in the MEBO group. The mRNA and protein expression of PI3K, Akt and mTOR in each group reached the peak on Day 5, the levels in the MEBO group were the highest (F ¼ 18.43, 19.97, 15.36, p < 0.05). On Day 11, the expression levels in each group began to decline. Discussion and conclusions: In this study, we discussed the molecular mechanism of MEBT/MEBO promoting the repair of diabetic ulcer wounds through autophagy and PI3K-Akt-mTOR signalling pathway, which provides a new way for drug design in the future.

Moist Exposed Burn Ointment Effectiveness for Capecitabine Associated Grade II and III Hand Foot Syndrome on Stage III Colonic

“…Many studies reported the mechanism of MEBO, it could improved the microcirculation of the tissue, participate in neurovascularization of the wound and epithelial regeneration. The earlier clinical usage is for local the treatment of burn injury and ulcer including diabetic ulcer [4].…”

“…One theory proposed to explain capecitabine-associated HFS is that specialized skin cells (keratinocytes) might have upgraded levels of the enzyme thymidine phosphorylase. Another theory suggests that capecitabine may be eliminated by the eccrine system (sweat secretion), and HFS is caused by an unknown mechanism related to the increased number of eccrine glands present in the hands and feet [3], [4], [6].…”

“…For the cases with Grade 1 HFS, the treatment continued using the original dose with the application of a skin barrier cream and MEBO. For Grade 2 HFS, the original dose was maintained with application of MEBO [4], [7], [9], [10].…”

Bioinformatics analysis combined with experiments to verify potential autophagy genes in wound healing

“…Studies have shown that autophagy is involved in the regulation of a number of cellular functions and is closely related to the development of diseases, such as cancer, aging, and trauma, and is currently a hotspot in research (6,7). Autophagy can reduce the production of inflammatory factors, induce wound angiogenesis, reepithelialization and scar formation in the process of wound healing, and can also improve the impaired cell activity in high glucose environment (8)(9)(10)(11). However, the mechanism of the role of autophagy in trauma healing is still unclear.…”

Research advances in prevention and treatment of burn wound deepening in early stage

“…Moistened exposure burn therapy (MEBT) is a local treatment method that treats burn wounds with moist exposed burn ointment(MEBO) and exposes burn tissue to repair and regeneration in a physiologically moist environment. MEBO applied to the wound surface can effectively improve the microcirculation in the zone of stasis and prevent the progressive necrosis of the tissue nearby (41). The advantages of MEBT are as follows: First, MEBT can effectively improve the microcirculation of the wound, avoiding the formation of a large number of microthrombosis and reducing the degree of ischemia and hypoxia in the zone of stasis.…”