Effects of monoamine neuromediators on the growth-related variables of Escherichia coli K-12

Anuchin, A. M.; Chuvelev, D. I.; Kirovskaya, T. A.; Oleskin, Alexander V.

doi:10.1134/s0026261708060040

Cited by 21 publications

(13 citation statements)

References 6 publications

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“…High concentrations of SP (>10 −4 M) have previously been shown to display both direct and indirect antimicrobial activities on several bacteria including E. coli and P. aeruginosa, by acting as a weak cationic antimicrobial peptide (Kowalska et al 2002;Hansen et al 2006;Lesouhaitier et al 2009). Conversely, 5HT added to an E. coli K12 culture stimulates cell proliferation and biomass accumulation (Anuchin et al 2008), and the authors of this study showed a nonlinear concentration dependence curve probably due to a specific receptor-/sensor-dependent mechanism. 5HT was also found to enhance the growth and cellular aggregation of other bacterial species including Streptococcus faecalis or eukaryotic microorganisms, such as the yeast Candida guillermondii (Strakhovskaia et al 1993).…”

Section: Discussionmentioning

confidence: 97%

The pathogenic potential of Pseudomonas fluorescens MFN1032 on enterocytes can be modulated by serotonin, substance P and epinephrine

et al. 2015

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Pseudomonas fluorescens is a commensal bacterium present at low level in the human digestive tract that has also been reported in many clinical samples (blood, urinary tract, skin, lung, etc.) and sometimes associated with acute opportunistic infections. It has recently been found that the human β-defensin-2 can enhance the pathogenic potential of P. fluorescens. In this study, we evaluated the effect of other intestinal molecules (5HT, SP and Epi) on growth and virulence of the clinical strain P. fluorescens MFN1032. We found that P. fluorescens MFN1032 growth was not mainly affected by these factors, but several modifications in the virulence behavior of this bacterium were observed. 5HT, SP and Epi were able to modulate the motility of P. fluorescens MFN1032. 5HT and SP had an effect on pyoverdin production and IL-8 secretion, respectively. Infection of Caco-2/TC7 cells with P. fluorescens MFN1032 pretreated by SP or Epi enhanced the permeability of the monolayers and led to a partial delocalization of F-actin to the cytoplasm. These findings show that some intestinal molecules can modulate the pathogenic potential of P. fluorescens MFN1032. We can hypothesize that this dialogue between the host and the human gut microbiota may participate in health and disease.

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Section: Discussionmentioning

confidence: 97%

The pathogenic potential of Pseudomonas fluorescens MFN1032 on enterocytes can be modulated by serotonin, substance P and epinephrine

et al. 2015

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“…Thus, the bioassay method that is based on the bacterial luminescence model can be applied for the evaluation of the biological effects of stimulatory and inhibitory concentrations of natural and synthetic neurochemicals. Biogenic amines produce analogous effects on biosensor cell luminescence and on the growth parameters and physiological characteristics of bacterial cells (see the authors' earlier research, [13,14]), This fact emphasizes that the information obtained with the bioluminescence test is of primary relevance to the physiological effects of neurotransmitters, including their impact on the human microbiota. Therefore, our test technique and the data obtained with its help seem to be of considerable importance with regard to future research concerning human physical and mental health.…”

Section: Results and Discusionmentioning

confidence: 90%

“…The serotonin effect on biosensor luminescence proved to be analogous to that on biomass accumulation, colony-forming unit (CFU) generation, and cell aggregation in E. coli. All these processes were stimulated by serotonin at concentrations below 20 µM and inihibited by higher serotonin concentrations [13,14]. Since high serotonin concentrations (> 50 µM) also suppressed the generation of membrane potential by bacterial membranes, the inhibitory effect of serotonin was attributed to its uncoupling activity with respect to membrane phosphorylation [14].…”

Section: Results and Discusionmentioning

confidence: 99%

“…Stimulatory activity with respect to microorganisms may be referred to as negative microbiota toxicity. Negative toxicity with regard to the bacterium Escherichia coli was revealed with nano-and micromolar concentrations of dopamine, norepinephrine, serotonin, and histamine [13]. Increasing the concentration of, e.g., serotonin results in changing the toxicity sign: it becomes positive, i.e., high serotonin concentration suppress, and not stimulate, E. coli growth [14].…”

Section: Introductionmentioning

confidence: 99%

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Testing Neurotransmitters for Toxicity with a Luminescent Biosensor: Implications for Microbial Endocrinology

Oleskin

Сорокина

Zarubina

et al. 2017

J. Pharm. Nutr. Sci.

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Background: The human organism is a complex superorganism including numerous eukaryotic, eubacterial, and archaean cells. The qualitative and quantitative assessment of the microbiota toxicity of chemical agents, i.e., their inhibitory effects on the microbial inhabitants of the human organism in health and disease, seems to hold much value in this context. In this work, a bacterial luminescence-based express test system for microbiota toxicity is applied to neurotransmitters such as serotonin, dopamine, norepinephrine, and histamine. Methods:The biosensor was based on a GM Escherichia coli K12 strain (TGI) that contained the lux operon of the luminescent soil bacterium Photorhabdus luminescencens ZMI. The biosensor was exposed to the action of the tested neurotransmitters for 5 to 60 minutes The intensity of bacterial luminescence (counts . sec -1 ) was monitored in the control and the experimental samples with a Biotoks 6 ms luminometer (Russia); the toxicity index (T) of the neurotransmitters was determined.Results: A marked toxic effect on bioluminescence was produced by serotonin, histamine, and dopamine at concentrations exceeding 80 µg/ml, 100 µg/ml, and 1 mg/ml, respectively. At lower concentration, these neurotransmitters were "negatively toxic", i.e. stimulatory in terms of the effect on bacterial luminescence. In contrast, norepinephrine inhibited luminescence at all concentrations tested. Conclusions:The bacterial luminescence-based testing method is applicable to the assessment of the destructive and stimulatory effects of neurotransmitters; the data obtained are of microbiological and clinical relevance.

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“…The crystal structure of histamine dehydrogenase isolated from Nocardioides simplex, catalyzing the oxidative deamination of histamine to imidazole acetaldehyde has been described recently (14). Even though histamine metabolic pathways have not been identified in E. coli so far, exogenous histamine has been reported to stimulate proliferation and biomass accumulation during the late lag and early exponential growth phases of E. coli K-12 strain in a nonlinear concentrationdependent fashion (65). Additionally, exogenous histamine has been implicated in the AtoSC-regulated cPHB biosynthesis (3).…”

Section: Histamine Modulation Of Atosc Signalingmentioning

confidence: 99%

Histamine in two component system-mediated bacterial signaling

Marina¹

2012

Front Biosci

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Histamine is a key mediator governing vital cellular processes in mammals beyond its decisive role in inflammation. Recent evidence implies additional actions in both eukaryotes and prokaryotes. Besides its function in host defense against bacterial infections, histamine elicits largely undefined actions in microorganisms that may contribute to bacteria-host interactions. Bacterial proliferation and adaptation are governed by sophisticated signal transduction networks, including the versatile two-component systems (TCSs) that comprise sensor histidine kinases and response regulators and rely on phosphotransfer mechanisms to exert their modulatory function. The AtoSC TCS regulates fundamental cellular processes such as short-chain fatty acid metabolism, poly-(R)-3-hydroxybutyrate (cPHB) biosynthesis and chemotaxis in Escherichia coli. The implication of exogenous histamine in the AtoSC-mediated cPHB biosynthesis and in E. coli chemotactic behavior is indicative of a putative function of histamine in bacterial physiology. The data raise questions on the significance of histamine actions in bacteria-host symbiosis, dysbiosis and pathogenicity as well as on the possible consequences upon therapeutic administration of histamine receptor-targeting agents and in particular ligands of the recently identified immunomodulatory H4 receptor.

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Effects of monoamine neuromediators on the growth-related variables of Escherichia coli K-12

Cited by 21 publications

References 6 publications

The pathogenic potential of Pseudomonas fluorescens MFN1032 on enterocytes can be modulated by serotonin, substance P and epinephrine

The pathogenic potential of Pseudomonas fluorescens MFN1032 on enterocytes can be modulated by serotonin, substance P and epinephrine

Testing Neurotransmitters for Toxicity with a Luminescent Biosensor: Implications for Microbial Endocrinology

Histamine in two component system-mediated bacterial signaling

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