Effects of morphology and pore size of mesoporous silicas on the efficiency of an immobilized enzyme

Kuo, Ping Chung; Lin, Zhi Xun; Wu, Tzi Yi; Hsu, Chun Han; Lin, Hong Ping; Wu, Tian Shung

doi:10.1039/d1ra01358k

Cited by 24 publications

(16 citation statements)

References 42 publications

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“…It was suggested that more protein could be adsorbed in the larger pores, although desorption is favored, and the dense arrangement hampers conformational flexibility with concomitant loss of activity [124]. This overall pattern had also been previously reported by Takimoto and co-workers [125] while immobilizing cellulase in mesoporous materials with pore sizes ranging from 5.4 to 11 nm. Kuo and co-workers also highlighted the relevance of pore morphology in enzyme performance.…”

Section: Geometry and Pore Size And Morphology Of The Carriersupporting

confidence: 76%

Enzyme Immobilization and Co-Immobilization: Main Framework, Advances and Some Applications

et al. 2022

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Enzymes are outstanding (bio)catalysts, not solely on account of their ability to increase reaction rates by up to several orders of magnitude but also for the high degree of substrate specificity, regiospecificity and stereospecificity. The use and development of enzymes as robust biocatalysts is one of the main challenges in biotechnology. However, despite the high specificities and turnover of enzymes, there are also drawbacks. At the industrial level, these drawbacks are typically overcome by resorting to immobilized enzymes to enhance stability. Immobilization of biocatalysts allows their reuse, increases stability, facilitates process control, eases product recovery, and enhances product yield and quality. This is especially important for expensive enzymes, for those obtained in low fermentation yield and with relatively low activity. This review provides an integrated perspective on (multi)enzyme immobilization that abridges a critical evaluation of immobilization methods and carriers, biocatalyst metrics, impact of key carrier features on biocatalyst performance, trends towards miniaturization and detailed illustrative examples that are representative of biocatalytic applications promoting sustainability.

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Section: Geometry and Pore Size And Morphology Of The Carriersupporting

confidence: 76%

Enzyme Immobilization and Co-Immobilization: Main Framework, Advances and Some Applications

et al. 2022

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“…SBA-15 is a hexagonal mesoporous silica ( Figure 3 a) with a high specific surface area and pore size that is often used for enzyme immobilization. Kuo et al [ 79 ] immobilized Streptomyces griseus HUT 6037 in three different mesoporous matrices: mesoporous silica film, mesocellular foam, and rod-like SBA-15. Their study demonstrated that all three immobilized materials exhibited more than 90% enzyme loading at optimal pH with efficient performance and reusability, indicating that the mesoporous materials improved the chemistry and stability of the enzyme.…”

Section: Overview Of Immobilization Technologymentioning

confidence: 99%

Immobilization of Biomass Materials for Removal of Refractory Organic Pollutants from Wastewater

Liu

Yang

Zhang

et al. 2022

IJERPH

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In the field of environmental science and engineering, microorganisms, enzymes and algae are promising biomass materials that can effectively degrade pollutants. However, problems such as poor environmental adaptability, recycling difficulties, and secondary pollution exist in the practical application of non-immobilized biomass materials. Biomass immobilization is a novel environmental remediation technology that can effectively solve these problems. Compared with non-immobilized biomass, immobilized biomass materials have the advantages of reusability and stability in terms of pH, temperature, handling, and storage. Many researchers have studied immobilization technology (i.e., methods, carriers, and biomass types) and its applications for removing refractory organic pollutants. Based on this, this paper reviews biomass immobilization technology, outlines the mechanisms and factors affecting the removal of refractory organic pollutants, and introduces the application of immobilized biomass materials as fillers for reactors in water purification. This review provides some practical references for the preparation and application of immobilized biomass materials and promotes further research and development to expand the application range of this material for water purification.

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“…Recently, Kuo et al investigated the effects of morphology and pore size of silica nanoparticles on the efficiency of enzyme immobilization using Streptomyces griseus HUT 6037 enzyme as a model enzyme and three different silica nanoparticles as supports. [18] It was reported that the loading capacity of the immobilized enzyme mainly depended on the surface charge of the particle, and the enzyme activity was related to the support type. Film-type mesoporous silica with short nanochannel length could maximize the accessibility of the immobilized enzyme to the environment, therefore achieving the highest activity.…”

Section: Enhanced Enzyme Activitymentioning

confidence: 99%

Silica‐based Nanoparticles for Enzyme Immobilization and Delivery

Zhao

Zhang

Yang

et al. 2022

Chemistry An Asian Journal

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Enzymes play an indispensable role in biosystems, catalyzing a variety of chemical and biochemical reactions with exceptionally high efficiency and selectivity. These features render them uniquely positioned in developing novel catalytic systems and therapeutics. However, their practical application is largely hindered by the vulnerability, low reusability and the inability to overcome the biological barriers of enzymes. Silica‐based nanoparticles (SNPs) are a classic family of nanomaterials with tunable physicochemical properties, making them ideal candidates to address the intrinsic shortcomings of natural enzymes. SNPs not only improve the activity and durability of enzymes, but also provide precise spatiotemporal control over their intracellular as well as systemic biodistributions for boosting the catalytic outcome. Herein, the recent progress in SNPs for enzyme immobilization and delivery is summarized. The therapeutic applications, including cancer therapy and bacterial inhibition, are particularly highlighted. Our perspectives in this field, including current challenges and possible future research directions are also provided.

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Effects of morphology and pore size of mesoporous silicas on the efficiency of an immobilized enzyme

Abstract: Mesoporous silica films were used as supports with high loading capacity and enzyme activity.

Cited by 24 publications

References 42 publications

Enzyme Immobilization and Co-Immobilization: Main Framework, Advances and Some Applications

Enzyme Immobilization and Co-Immobilization: Main Framework, Advances and Some Applications

Immobilization of Biomass Materials for Removal of Refractory Organic Pollutants from Wastewater

Silica‐based Nanoparticles for Enzyme Immobilization and Delivery

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