Effects of multi-components released from S-PRG filler on the activities of human dental pulp-derived stem cells

Abstract

Numerous studies have shown that the sustained release of ions from dental restorative materials have acid buffering capacity, prevents tooth enamel demineralization, and inhibits bacterial adhesion. Herein, the release behavior and bioresponsiveness of ions released from surface pre-reacted glass-ionomer (S-PRG) fillers were investigated in different types of media based on human dental pulp-derived stem cell (hDPSC) responses. The hDPSCs were cultured for 1-7 days in S-PRG eluates diluted with varying amounts of cell culture media. S-PRG released several types of ions, such as F − , Sr 2+ , Na + , Al 3+ , BO3 3− , and SiO3 2− . The balance of eluted ions differed depending on the dilution and solvent, which in turn affected the cytotoxicity, cell morphology, cell proliferation, and alkane phosphatase activity of hDPSCs, among other properties. The results suggest that tailored S-PRG filler eluates could be designed and prepared for application in dental practice.

Bioactivity of human dental pulp-derived stem cells with boron-controlled S-PRG filler eluate by anion exchange

“…These results suggest that ALP activity increases according to the B concentration. Ishigure et al 24) reported that ALP activity of hDPSC increased with the addition of the S-PRG filler eluate, which contained a higher amount of B than other ions. The results in this study suggest that S-PRG filler-derived B contributes to an increase in ALP activity.…”

“…In addition, the comparison between E3 and the control medium showed the cell proliferation effect and ALP activity decreased in E3 compared with the control, despite that the concentration of B in the medium was almost the same. Ishigure et al 24) reported that the hDPSC proliferation was promoted in the medium where the S-PRG filler eluate has been added, probably due to the larger amount of Sr compared with other ions. As above, the presence of the cell proliferation effect and ALP activity decreased in E3 in comparison with the control medium.…”

“…Regarding F, an ion meter equipped with an ion electrode (Fluoride electrode, Model 9609BN; pH/ion meter, Model 720A; Orion Research, Beverly, MA, USA) was used to measure F − concentration. The quantitative evaluation of the above was conducted according to the measurement methods of Ishigure et al 24) B, Si, F, Al, and Sr are S-PRG filler-derived components in the eluate, while K, Ca, and Mg are culture-derived components. Na is derived from both S-PRG filler and a culture medium.…”

“…Ishigure et al 24) prepared multiple S-PRG filler eluate solutions with a different component ratio of the eluates by changing the solvent amount in the elute preparation. They indicated that a culture medium to which was added an S-PRG filler eluate rich in boron enhanced alkaline phosphatase (ALP) activity in human dental pulp-derived stem cells (hDPSC).…”

Effect of Zinc Oxide Incorporation on the Antibacterial, Physicochemical, and Mechanical Properties of Pit and Fissure Sealants

“…Currently, composite resins containing bioactive fillers based on surface pre-reacted glass (S-PRG) technology, which can continuously release multiple ions such as fluoride (F − ), sodium (Na + ), strontium (Sr 2+ ), silicate (SiO 3 2− ), borate (BO 3 3− ), and aluminum (Al 3+ ) ions, are being used as pit and fissure sealants. The mechanism underlying S-PRG technology is known to involve an acid–base reaction between fluoroboraluminosilicate glass and polycarboxylic acid, resulting in material biofunctionality [ 8 ]. Previous research has demonstrated that dental products containing S-PRG fillers can prevent tooth enamel demineralization [ 9 ] and provide acid-buffering ability [ 10 ] because of the constant release of these ions.…”