Effects of multiple Bt proteins and GNA lectin on in vitro-reared honey bee larvae

Hendriksma, Harmen P.; Härtel, Stephan; Babendreier, D.; Ohe, Werner von der; Steffan‐Dewenter, Ingolf

doi:10.1007/s13592-012-0123-3

Cited by 30 publications

(19 citation statements)

References 42 publications

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“…1). The results are in line with recent tests on Cry1Ac/CpTI cotton pollen [18], [41], [46], stacked Bt maize pollen [43] or purified Bt proteins [44], [45].…”

Section: Discussionsupporting

confidence: 90%

“…Neither larval nor adult honey bees have ever shown lethality when exposed to Bt proteins [44], [45], and our data also suggest that synergistic effects of stacking Bt proteins at plant-produced levels are unlikely a be a risk to emerging adult bees. At a realistic exposure dose, the 7 day survivorship of Bt-pollen treated bees in our study was similar to that of bees exposed to the conventional cotton pollen (Fig.…”

Section: Discussionmentioning

confidence: 50%

“…Comparing Cry1 with transgenic protease inhibitors in many studies, only the latter impacted the feeding behavior [50]–[53]. Better knowledge about the sublethal risks associated with ZMSJ and ZMKCKC pollen for honey bees may also be obtained studying the effects of pollen [13] or multiple Bt proteins [45] on larval development.…”

Section: Discussionmentioning

confidence: 99%

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Impact of Single and Stacked Insect-Resistant Bt-Cotton on the Honey Bee and Silkworm

Niu

Mannakkara

et al. 2013

PLoS ONE

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Transgenic insect-resistant cotton (Bt cotton) has been extensively planted in China, but its effects on non-targeted insect species such as the economically important honey bee (Apis mellifera) and silkworm (Bombyx mori) currently are unknown. In this study, pollen from two Bt cotton cultivars, one expressing Cry1Ac/EPSPS and the other expressing Cry1Ac/Cry2Ab, were used to evaluate the effects of Bt cotton on adult honey bees and silkworm larvae. Laboratory feeding studies showed no adverse effects on the survival, cumulative consumption, and total hemocyte count (THC) of A. mellifera fed with Bt pollen for 7 days. No effects on the survival or development of B. mori larvae were observed either. A marginally significant difference between Cry1Ac/Cry2Ab cotton and the conventional cotton on the THC of the 3rd day of 5th B. mori instar larvae was observed only at the two highest pollen densities (approximately 900 and 8000 grains/cm2), which are much higher than the pollen deposition that occurs under normal field conditions. The results of this study show that pollen of the tested Bt cotton varieties carried no lethal or sublethal risk for A. mellifera, and the risk for B. mori was negligible.

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“…1). The results are in line with recent tests on Cry1Ac/CpTI cotton pollen [18], [41], [46], stacked Bt maize pollen [43] or purified Bt proteins [44], [45].…”

Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 50%

See 1 more Smart Citation

Impact of Single and Stacked Insect-Resistant Bt-Cotton on the Honey Bee and Silkworm

Niu

Mannakkara

et al. 2013

PLoS ONE

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“…Transgenic Bt cry1Ab or cry1F corn pollen did not pose a threat to honey bee larval development (Hanley et al, 2003). Cry1A.105, Cry2Ab2 and Cry3Bb1 proteins showed no adverse effects on bee larvae using controlled in vitro larvae rearing (Hendriksma et al, 2012). Bees in general may be exposed to transgene products via pollen.…”

Section: Discussionmentioning

confidence: 94%

Effects of Btcry1Ahcorn pollen on immature worker survival and development ofApis cerana cerana

Dai

Zhou

Zhang

et al. 2015

Journal of Apicultural Research

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2015): Effects of Bt cry1Ah corn pollen on immature worker survival and development of Apis cerana cerana, Journal of Apicultural Research,The honey bee may be exposed to insecticidal proteins from transgenic plants via pollen. An assessment of the impact of such exposures on the honey bee is an essential part of the risk assessment process for transgenic Bacillus thuringiensis (Bt) corn. The effects of dietary transgenic Bt corn pollen on honey bee immature workers of Apis cerana cerana were examined by feeding trials in colonies. Four-to six-day-old honey bee worker larvae were fed various pollens (cry1Ah corn pollen, regular corn pollen, mixed bee pollen and control) and then sampled to record their survival and development. There were no significant differences in capping rate, emergence rate and immature stage among treatments. Our studies suggest that cry1Ah corn pollen carries no risk for the survival and development of A. c. cerana immature workers.Efectos del polen de maíz Bt cry1Ah en la supervivencia y desarrollo de obreras inmaduras de Apis cerana cerana Las abejas podrían estar expuestas a las proteínas de insecticidas de plantas transgénicas a través de su polen. Por ello, la valoració n del impacto de dicha exposició n es esencial para entender el riesgo que supone para las abejas el maíz transgénico Bacillus thuringiensis. El efecto dietético del polen del maíz transgénico Bt en las obreras inmaduras de Apis cerana cerana fue examinado mediante ensayos de alimentació n en colonias. Se alimentaron larvas de obreras de entre 4 y 6 días con varios tipos de polen (polen de maíz cry1Ah, polen de maíz normal, mezcla de polen de abeja y un control), luego fueron muestreadas para registrar los datos de supervivencia y desarrollo. No se encontraron diferencias significativas en las tasas de operculamiento, emergencia y nivel de inmadurez entre los tratamientos. Por tanto, el estudio sugiere que el polen del maíz cry1Ah no supone un riesgo para la supervivencia y desarrollo de las obreras inmaduras de Apis cerana cerana.

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“…In an in vitro-rearing study that tested only a single amount of transgenic pollen (2 mg; Hendriksma et al 2011b), no negative effect of transgenic pollen on prepupal weight was detected. Hendriksma et al (2012) could also show that the exposure to the same purified Bt proteins, at 186 times the estimated environmental concentration (EEC), did not affect the prepupal weight of in vitro-reared larvae. This underlines the importance of directly using the transgenic plant material and testing it in a dose-depending manor.…”

Section: Prepupal Weightmentioning

confidence: 96%

Testing dose-dependent effects of stacked Bt maize pollen on in vitro-reared honey bee larvae

2015

Self Cite

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-In agricultural landscapes all over the world, honey bees are exposed to genetically modified (GM) pollen. Stacked Bt-maize varieties combine multiple Bt proteins against different insect taxa with herbicide resistance. To test for potential effects on non-target organisms, we conducted an in vitro larvae-rearing experiment where we fed increasing amounts of stacked Bt pollen to honey bee larvae. Bt pollen was equally well digested as two control maize varieties. No dose-dependent effects of Bt maize were detected in terms of survival and developmental delay. However, for prepupal weight we did find a dose-dependent response, suggesting a pleiotropic effect. Comparing this finding with the literature we conclude that the found effect is not likely to occur in a field situation. We could however show that dose-dependent effects are detectable. Our results underline the importance of testing dose-depending effects of GM plant material in an environmental risk assessment (ERA).environmental risk assessment / transgenic pollen / Apis mellifera / ecotoxicology / sub-lethal effects

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Effects of multiple Bt proteins and GNA lectin on in vitro-reared honey bee larvae

Cited by 30 publications

References 42 publications

Impact of Single and Stacked Insect-Resistant Bt-Cotton on the Honey Bee and Silkworm

Impact of Single and Stacked Insect-Resistant Bt-Cotton on the Honey Bee and Silkworm

Effects of Btcry1Ahcorn pollen on immature worker survival and development ofApis cerana cerana

Testing dose-dependent effects of stacked Bt maize pollen on in vitro-reared honey bee larvae

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