Effects of Multiple Electrical Field Exposures on Cyprinid Embryo Survival

Abstract

The effects of electricity on fish embryo survival during sampling events have been previously researched, demonstrating that survival is affected by voltage gradient, current type, exposure duration, developmental stage, and egg diameter. However, the effects of multiple electrical field exposures on fish embryos, such as during sampling depletions, have not been investigated. We conducted multiple electrical field exposures over a range of voltage gradients and resting intervals to determine potential effects on zebrafish Danio rerio embryos during epiboly. However, multiple electrical field exposures had no effect on zebrafish embryo survival, and the only factor to significantly decrease survival regardless of the number of exposures was a voltage gradient of 16 V/cm. Since zebrafish are widely used as model cyprinids, our results suggest that multiple electrical field exposures during electrofishing depletions are not likely to significantly increase cyprinid fish embryo mortality. Received March 1, 2012; accepted May 23, 2012

Impact of Pulsed Direct Current on Embryos, Larvae, and Young Juveniles of Atlantic Cod and its Implications for Electrotrawling of Brown Shrimp

“…Therefore, a greater proportion of abnormal Atlantic Cod larvae hatching from the eggs of stressed females may be produced (Morgan et al 1999). Although multiple exposures with intervals of 1-5 min did not appear to cause major harm to Zebrafish Danio rerio embryos (Nutile et al 2012), research on the effects of electrofishing on young marine organisms is limited to single-exposure events. Information on the impact of multiple exposures is important, as certain fishing grounds, including spawning areas, may be fished intensely during particular seasonal periods (Piet and Hintzen 2012;van Denderen et al 2015).…”

Pulse Trawling: The Impact of Pulsed Direct Current on Early Life Stages of Sole Solea solea

“…; Nutile et al. ). Also, survival of Atlantic Cod embryos at epiboly, which occurred at 5 DPF, was not negatively affected when embryos were exposed to the same electrical pulse (Desender et al.…”

“…At 2 DPF the Sole embryo is in epiboly. A thin layer of embryonal tissue is growing over the yolk at this stage, which is considered to be the most sensitive stage during development in different species (Dwyer et al 1993;Muth and Rupert 1997;Roach 1999;Henry and Grizzle 2004;Bohl et al 2010;Nutile et al 2012). Also, survival of Atlantic Cod embryos at epiboly, which occurred at 5 DPF, was not negatively affected when embryos were exposed to the same electrical pulse (Desender et al 2017).…”

Effect of Waveform and Voltage Gradient on the Survival of Electroshocked Steelhead Embryos and Larvae

“…The exposure tank was constructed from a 9.5-L glass aquarium (35 × 14 × 22 cm; cf. Nutile et al 2012). Two 3.2mm-thick aluminum plates that served as electrodes were placed into the tank 10 cm apart in a parallel orientation.…”

“…Groups of embryos or larvae (n = 14-19) at the desired developmental stage were selected from rearing trays and placed into 4.8-cm-diameter "exposure" baskets made with 500-µm mesh (cf. Nutile et al 2012). These baskets were submerged in the exposure tank filled with 5 L of well water (temperature = 10.6-13.4°C; specific conductivity = 340-360 µS/cm) and then were shocked or shamshocked for 30 s. After the baskets of embryos or alevins were shocked, they were transferred into rearing trays.…”