Effects of Multisolute Steric Interactions on Membrane Partition Coefficients

Lazzara, Matthew J.; Blankschtein, Daniel; Deen, William M.

doi:10.1006/jcis.2000.6800

Cited by 52 publications

(83 citation statements)

References 32 publications

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“…Since we wanted to model the DNA fragments as cylinders instead of spheres, the theoretical approach developed by the Blankschtein and Deen research groups (Lazzara et al, 2000;Nikas et al, 1992) for modeling partitioning was extended to incorporate cylinder-cylinder, that is, DNA-micelle, excluded-volume interactions. Specifically, the partition coefficient of the DNA fragments is equal to the probability of the DNA fragments not intersecting micelles in the top phase divided by the same probability for the bottom phase.…”

Section: Theoretical Aspects Excluded-volume Theorymentioning

confidence: 99%

Concentration of mammalian genomic DNA using two‐phase aqueous micellar systems

Mashayekhi

Meyer

Shiigi

et al. 2008

Biotech & Bioengineering

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The concentration of biomarkers, such as DNA, prior to a subsequent detection step may facilitate the early detection of cancer, which could significantly increase chances for survival. In this study, the partitioning behavior of mammalian genomic DNA fragments in a two-phase aqueous micellar system was investigated using both experiment and theory. The micellar system was generated using the nonionic surfactant Triton X-114 and phosphate-buffered saline (PBS). Partition coefficients were measured under a variety of conditions and compared with our theoretical predictions. With this comparison, we demonstrated that the partitioning behavior of DNA fragments in this system is primarily driven by repulsive, steric, excluded-volume interactions that operate between the micelles and the DNA fragments, but is limited by the entrainment of micelle-poor, DNA-rich domains in the macroscopic micelle-rich phase. Furthermore, the volume ratio, that is, the volume of the top, micelle-poor phase divided by that of the bottom, micelle-rich phase, was manipulated to concentrate DNA fragments in the top phase. Specifically, by decreasing the volume ratio from 1 to 1/10, we demonstrated proof-of-principle that the concentration of DNA fragments in the top phase could be increased two- to nine-fold in a predictive manner.

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Section: Theoretical Aspects Excluded-volume Theorymentioning

confidence: 99%

Concentration of mammalian genomic DNA using two‐phase aqueous micellar systems

Mashayekhi

Meyer

Shiigi

et al. 2008

Biotech & Bioengineering

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“…In section 4, a comparison of results from Eqs. [3] and [17] is made using the conditions from the partitioning experiments described in Section 3.…”

Section: Effects Of Charge and Solute Concentrationmentioning

confidence: 99%

“…The predictions of Eq. [3] apply to infinitely dilute solute concentrations. Using density functional theory and Monte Carlo simulations, Fanti and Glandt (5,6) investigated the effect of solute concentration on the partitioning of spherical particles in gels.…”

Section: Introductionmentioning

confidence: 99%

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The Effect of Solute Concentration on Equilibrium Partitioning in Polymeric Gels

Gerhardt

Dungan

Phillips

2001

Journal of Colloid and Interface Science

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“…The distribution coefficient is an important parameter in size exclusion chromatography. Therefore, many efforts have been undertaken to predict this distribution coefficient from the size and shape of the solute(s) and the size, shape and concentration of the fibers or obstacles [8][9][10][11][12][13][14]. One of the first efforts has been undertaken by Ogston [8], who has derived a model for the distribution coefficient that is based on the available space fraction for a rigid spherical solute in a random distribution of long fibers.…”

Section: Distribution Coefficients In Size Exclusion Chromatographymentioning

confidence: 99%