Effects of Mycotoxins on Invertebrate Cells in Vitro

Quiot, J.M.; Vey, A.; Vago, C.

doi:10.1016/b978-0-12-007904-9.50013-7

Cited by 28 publications

(16 citation statements)

References 13 publications

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“…Although insect cell lines are used extensively as model to study the mechanism of action of the fungal metabolites (Dumas et al, 1994(Dumas et al, , 1996Liu et al, 1996;Quiot et al, 1985;Vey and Quiot, 1989), few data have been reported on the effects of fungal metabolites tested (Abado-B ecogn ee, 1999;Cal o et al, 2003;Logrieco et al, 1996;Vey et al, 1973). In particular Abado-B ecogn ee (1999) reported the toxic effects induced by DON, FB 1 , and MN on a lepidopteran Ploidia interpunctella wing cell line (IAL-PID-2) by MTT test, release of LDH, by MTT test, the values of IC 50 were of 2.8, 102, and 260 lM for DON, FB 1 , and MN, respectively.…”

Section: Resultsmentioning

confidence: 97%

“…The aim of this paper was to evaluate the sensitivity of the SF-9 cell line toward fungal metabolites. In the past (Gutleb et al, 2002;Quiot et al, 1985;Vey and Quiot, 1989) insect cell lines have been used to test toxins and some studies used the SF-9 cells in particular (Dumas et al, 1996;Liu et al, 1996). In those works the mode of action induced by fungal metabolites such as dextrusins, cyclosporin A, hirsutellin A was described, and no toxicological screening was carried out.…”

Section: Introductionmentioning

confidence: 99%

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Cytotoxicity of fungal metabolites to lepidopteran (Spodoptera frugiperda) cell line (SF-9)

Fornelli

Minervini

Logrieco

2004

Journal of Invertebrate Pathology

130

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Section: Resultsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Cytotoxicity of fungal metabolites to lepidopteran (Spodoptera frugiperda) cell line (SF-9)

Fornelli

Minervini

Logrieco

2004

Journal of Invertebrate Pathology

130

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“…They are particularly toxic to mammalian leukemia cells and spleen lymphocytes and have demonstrated anti-proliferative activity on mouse neoplasms in vitro (16). Destruxins A, B, and E have also been shown to have antiviral properties in insect and human cell lines (17)(18)(19). For example, Destruxin B has demonstrated a suppressive effect on hepatitis B surface antigen expression (20,21).…”

mentioning

confidence: 99%

Fungal Peptide Destruxin A Plays a Specific Role in Suppressing the Innate Immune Response in Drosophila melanogaster

Pal

Leger

2007

Journal of Biological Chemistry

182

119

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Destruxins are a class of insecticidal, anti-viral, and phytotoxic cyclic depsipeptides that are also studied for their toxicity to cancer cells. They are produced by various fungi, and a direct relationship has been established between Destruxin production and the virulence of the entomopathogen Metarhizium anisopliae. Aside from opening calcium channels, their in vivo mode of action during pathogenesis remains largely uncharacterized. To better understand the effects of a Destruxin, we looked at changes in gene expression following injection of Destruxin A into the fruit fly Drosophila melanogaster. Microarray results revealed reduced expression of various antimicrobial peptides that play a major role in the humoral immune response of the fly. Flies co-injected with a non-lethal dose of Destruxin A and the normally innocuous Gram-negative bacteria Escherichia coli, showed increased mortality and an accompanying increase in bacterial titers. Mortality due to sepsis was rescued through ectopic activation of components in the IMD pathway, one of two signal transduction pathways that are responsible for antimicrobial peptide induction. These results demonstrate a novel role for Destruxin A in specific suppression of the humoral immune response in insects.

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“…The polyploidization, micronucleation and other nuclear characteristics here described for the BTC-32 line should be taken into account when intending to use this culture for assays of the arthropod phase of Trypanosoma cruzi (Pudney & Lanar, 1977) and for studies of the mode of action of entomopathogenic fungi proposed for insect biological management (Quiot et al, 1985).…”

Section: Discussionmentioning

confidence: 99%

Polyploidization of the BTC-32 cell line from Triatoma infestans (Hemiptera, Reduviidae)

Mello

Pudney

1987

Genetica

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The cytophotometric evaluation of the Feulgen-DNA content of the BTC-32 cells at passage 160 after 6 days of growth demonstrated that polyploidy is relatively frequent in this cell line. 4C values were assumed to pertain to diploid nuclei at the S or G2 phases but also to polyploid nuclei at the G1 phase. Polyploidy in 4C nuclei is assumed to be attained by endomitosis. However, there are morphological indications that polyploidization in cells with an 8C-128C Feulgen-DNA content could result from cellular and nuclear fusions, possibly mediated by viral particles present in the cell culture. Micronucleation was also frequent; it was assumed to be promoted by viral action or deficiency in some culture medium nutrient. These nuclear characteristics should be considered when using the BTC-32 cell line for monitoring the action of infective agents or their products.

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Effects of Mycotoxins on Invertebrate Cells in Vitro

Cited by 28 publications

References 13 publications

Cytotoxicity of fungal metabolites to lepidopteran (Spodoptera frugiperda) cell line (SF-9)

Cytotoxicity of fungal metabolites to lepidopteran (Spodoptera frugiperda) cell line (SF-9)

Fungal Peptide Destruxin A Plays a Specific Role in Suppressing the Innate Immune Response in Drosophila melanogaster

Polyploidization of the BTC-32 cell line from Triatoma infestans (Hemiptera, Reduviidae)

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