Effects of n-3 Polyunsaturated Fatty Acid-Enriched Hen Egg Consumption on the Inflammatory Biomarkers and Microvascular Function in Patients with Acute and Chronic Coronary Syndrome—A Randomized Study

Ćurić, Željka Breškić; Masle, Ana Marija; Kibel, Aleksandar; Selthofer-Relatić, Kristina; Stupin, Ana; Mihaljević, Zrinka; Jukić, Ivana; Stupin, Marko; Matić, Anita; Kozina, Nataša; Šušnjara, Petar; Juranić, Brankica; Kolobarić, Nikolina; Šerić, Vatroslav; Drenjančević, Ines

doi:10.3390/biology10080774

Cited by 7 publications

(17 citation statements)

References 47 publications

(51 reference statements)

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“…This was accompanied by a shift in a positive correlation between TNF-α and ICAM-1 in control conditions towards their significant negative correlation after consumption of enriched hen eggs, as well as increased expression of nNOS. Previously, we have demonstrated moderate anti-inflammatory effects of n-3 PUFAs enriched functional food in acute and chronic cardiovascular patients [33] and its cardioprotective and inflammation-resolving effect in the healthy adult population [8,10], which is further supported by results of other studies on n-3 PUFAs supplementation [34][35][36].…”

Section: Discussionsupporting

confidence: 67%

Consumption of Hen Eggs Enriched with n-3 Polyunsaturated Fatty Acids, Selenium, Vitamin E and Lutein Incites Anti-Inflammatory Conditions in Young, Healthy Participants — A Randomized Study

Šušnjara¹,

Kolobarić²,

Matić³

et al. 2022

Front. Biosci. (Landmark Ed)

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Background: Dietary supplementation with compounds that possess antioxidant and anti-inflammatory properties (n-3 polyunsaturated fatty acids (PUFAs), selenium, vitamin E, lutein), has been shown to positively correlate with improvements in chronic conditions, although understanding of these combined effects in healthy humans is limited. The study aimed to evaluate the effects of enriched eggs consumption on oxidative status and inflammatory conditions in healthy volunteers. We hypothesized that a three-week diet containing enriched eggs can alter the immune response of healthy adults towards anti-inflammatory conditions. Methods: 34 participants consumed 3 hard-boiled hen eggs per day (21 days): Control group-regular hen eggs (n-3 PUFAs = 438 mg, selenium = 0.054 mg, lutein = 0.330 mg and vitamin E = 1.785 mg) (N = 14); 4Nutri group-hen eggs enriched with 4 nutrients (n-3 PUFAs = 1026 mg, selenium = 0.06 mg, lutein = 1.85 mg and vitamin E = 3.29 mg) (N = 20). Samples were taken before and after the protocol. Serum concentrations of lipid mediators and cytokines were measured with enzyme-linked immunosorbent assay (ELISA) and antibody-based, magnetic bead reagent kits on the Luminex platform, respectively. Serum oxidative stress and antioxidant capacity were measured using standardized methods, while gene expression in peripheral blood mononuclear cells (PBMCs) was measured via real-time PCR. Results: Decreased serum levels of pro-inflammatory interleukin 17A (IL-17A) and an increased neuronal nitric oxide synthase (nNOS) expression in the 4Nutri group, together with alteration of metabolites produced via cyclooxygenase (COX) pathways in the Control group, suggest a shift towards anti-inflammatory conditions in participants who consumed enriched hen eggs. Conclusions: Present results suggest that the combined action of n-3 PUFAs and antioxidants may have a protective role in resting, non-inflammatory conditions. Clinical Trial Registration: NCT04564690.

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Section: Discussionsupporting

confidence: 67%

Consumption of Hen Eggs Enriched with n-3 Polyunsaturated Fatty Acids, Selenium, Vitamin E and Lutein Incites Anti-Inflammatory Conditions in Young, Healthy Participants — A Randomized Study

Šušnjara¹,

Kolobarić²,

Matić³

et al. 2022

Front. Biosci. (Landmark Ed)

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“…Measurement of microvascular blood ow and reactivity tests were performed in all subjects using laser Doppler owmetry (LDF) (MoorVMS-LDF, Axminster, UK), before and after corresponding diet protocols, as previously described [8,11]. Endothelium-dependent vasodilation of skin microcirculation was tested by measurement of microvascular response to vascular occlusion (post-occlusive reactive hyperemia, PORH) and to iontophoresis of acetylcholine (acetylcholine induced dilation, AChID), while endotheliumindependent vasodilation was assessed by iontophoresis of sodium-nitroprusside (sodium nitroprusside induced dilation, SNPID).…”

Section: Assessment Of Microvascular Endothelium-dependent and Endoth...mentioning

confidence: 99%

“…n-3 PUFAs enriched hen eggs) may improve microvascular endothelium-dependent vasodilation in healthy young individuals and in patients with metabolic diseases [5,7,10]. Importantly, consumption of regular and n-3 PUFAs enriched hen eggs do not have negative effects on any of the measured biological and functional vascular parameters in patients with cardiovascular diseases, as well as in healthy young subjects, suggesting that eggs can be safely consumed in the daily diet [7,11].…”

mentioning

confidence: 99%

Consumption of nutritionally enriched hen eggs enhances endothelium-dependent vasodilation via cyclooygenases’ metabolites in healthy young persons - A randomized study

Šušnjara

Mihaljević

Stupin

et al. 2022

Preprint

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Background Present study aimed to evaluate the effects of enriched hen eggs consumption on the endothelium-dependent vasodilation (EDV) and the role of cyclooxygenases in EDV in microcirculation of young healthy individuals. Methods Participants consumed 3 eggs per day for 3 weeks: Control group (n = 14) consumed regular hen eggs (approximately 0.330 mg of lutein, 1.785 mg of vitamin E, 0.054 mg of selenium and 438 mg of n-3 PUFAs daily) and Nutri4 group (n = 20) consumed enriched eggs (approximately 1.85 mg of lutein, 0.06 mg of selenium, 3.29 mg of vitamin E, and 1026 mg of n-3 PUFAs daily). Skin microvascular blood flow in response to EDV (post-occlusive reactive hyperemia (PORH) and iontophoresis of acetylcholine (AChID)) and sodium nitroprusside (SNPID; endothelium-independent) were assessed by laser Doppler flowmetry before and after dietary protocol, with or without 100 mg of indomethacin perorally. Arterial blood pressure, heart rate, serum lipid and liver enzymes, anthropometric measurements, protein expression of COX-1, COX-2, nNOS, iNOS, and eNOS were measured before and after dietary protocol. Results PORH and AChID were significantly enhanced, and SNPID remained unchanged in Nutri4 group, while none was changed in Control group following respective diet. PORH was decreased after administration of indomethacin in Nutri4 after dietary protocol. Protein expression of COX-2 was significantly higher in the Nutri4 group compared to Control group after dietary protocol. Conclusion Consumption of enriched eggs improves microvascular EDV in healthy young subjects. Results suggest involvement of n-3 PUFAs metabolites via the cyclooxygenases pathway in enhanced reactive hyperemia. Trial registration : The study was registered on the Clinical trial under the title: Effect of Enriched QUARTET® Hen Eggs on Cardiovascular Function in Cardiovascular Patients and Healthy Individuals. NCT number: NCT04564690 on September 2020 (https://clinicaltrials.gov/ct2/show/NCT04564690).

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“…Measuring EDV is important because it can provide insight into the health of blood vessels and endothelial function [ 7 , 8 ]. Endothelial dysfunction, which can manifest as reduced EDV, is associated with various diseases including atherosclerosis, hypertension, diabetes, and cardiovascular disease [ 9 ]. Furthermore, the increased proportion of EPA and DHA in the diet increases vasodilator mechanisms in the microcirculation of the forearm, which can contribute to lowering blood pressure [ 5 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

“…Evidence is accumulating that the consumption of n -3 PUFAs in a form of functional foods (e.g., n -3 PUFA-enriched hen eggs) may improve microvascular endothelium-dependent vasodilation in healthy young individuals and in patients with metabolic diseases [ 5 , 6 , 7 ]. Generally, the investigation of the effects of PUFAs has used doses ranging from 1 to 4 g per day, but the optimal dose can depend on the specific health condition being treated [ 6 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Consumption of Nutritionally Enriched Hen Eggs Enhances Endothelium-Dependent Vasodilation via Cyclooxygenase Metabolites in Healthy Young People—A Randomized Study

et al. 2023

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Objective: The present study aimed to evaluate the effects of enriched hen egg consumption on endothelium-dependent vasodilation (EDV) and the role of cyclooxygenases in EDV in the microcirculation of young healthy individuals. This study hypothesizes that Nutri4 eggs will improve endothelial function, which will be manifested by changes in microcirculatory flow measured by a laser Doppler flowmeter (LDF) during reactive hyperemia in response to vascular occlusion, in which n-3 PUFA plays an important role as well as its degradation pathway by cyclooxygenases. Materials and Methods: Participants consumed three eggs per day for three weeks: The control group (CTRL, n = 14) consumed regular hen eggs (approximately 0.330 mg of lutein, 1.785 mg of vitamin E, 0.054 mg of selenium and 438 mg of n-3 PUFAs daily) and Nutri4 group (n = 20) consumed enriched eggs (approximately 1.85 mg of lutein, 0.06 mg of selenium, 3.29 mg of vitamin E, and 1026 mg of n-3 PUFAs daily). Skin microvascular blood flow in response to EDV (post-occlusive reactive hyperemia (PORH) and iontophoresis of acetylcholine (AChID)) and sodium nitroprusside (SNPID; endothelium-independent) was assessed by laser Doppler flowmetry before and after dietary protocol and in a separate group of participants who were administered perorally 100 mg of indomethacin before microvascular response assessment. Arterial blood pressure, heart rate, serum lipid, and liver enzymes, anthropometric measurements, protein expression of cyclooxygenase 1 (COX-1), cyclooxygenase 2 (COX-2), neuronal nitric oxide synthases (nNOS), inducible nitric oxide synthases (iNOS), and endothelial nitric oxide synthases (eNOS) were measured before and after dietary protocol. Results: PORH and AChID were significantly enhanced, and SNPID remained unchanged in the Nutri4 group, while none was changed in the CTRL following a respective diet. PORH decreased after administration of indomethacin in Nutri4 after dietary protocol. Protein expression of COX-2 was significantly higher in the Nutri4 group compared to the CTRL after the dietary protocol. Conclusion: Consumption of enriched eggs improves microvascular EDV in healthy young subjects. Results suggest an element of n-3 PUFAs metabolites via the cyclooxygenases pathway in enhanced reactive hyperemia.

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Effects of n-3 Polyunsaturated Fatty Acid-Enriched Hen Egg Consumption on the Inflammatory Biomarkers and Microvascular Function in Patients with Acute and Chronic Coronary Syndrome—A Randomized Study

Cited by 7 publications

References 47 publications

Consumption of Hen Eggs Enriched with n-3 Polyunsaturated Fatty Acids, Selenium, Vitamin E and Lutein Incites Anti-Inflammatory Conditions in Young, Healthy Participants — A Randomized Study

Consumption of Hen Eggs Enriched with n-3 Polyunsaturated Fatty Acids, Selenium, Vitamin E and Lutein Incites Anti-Inflammatory Conditions in Young, Healthy Participants — A Randomized Study

Consumption of nutritionally enriched hen eggs enhances endothelium-dependent vasodilation via cyclooygenases’ metabolites in healthy young persons - A randomized study

Consumption of Nutritionally Enriched Hen Eggs Enhances Endothelium-Dependent Vasodilation via Cyclooxygenase Metabolites in Healthy Young People—A Randomized Study

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