Effects of NaCl on protein profiles of tetraploid and hexaploid wheat species and their diploid wild progenitors

Abstract

The soluble proteins extracted from the first leaf tissues of cultivated tetraploid (Triticum durum Desf., genome AB) and hexaploid (T. aestivum L., genome ABD) wheat species and their diploid wild progenitors [T. monococcum L. (A), Aegilops speltoides Tausch (B), and Aegilops tauschii Cosson (D)] exposed to 100 mmol/l NaCl stress were separated by two-dimensional (2D) gel electrophoresis. There was no newly synthesized protein in the NaCl treatment compared to the control treatment in all species. However, protein profiles showed some differences among species. Most of these proteins had acidic character; their isoelectric point (pI = pH − acidity of proteins) ranged between 5.1-6.9 and low-mol weight (LMW) between 20.3-30.6 kDa. Salt stress caused some proteins to increase or decrease. In the same MW and pI, 11 LMW and 3 intermediate-mol weight (IMW,.4 kDa) proteins increased and/or decreased in amounts were common between at least two species. The remarkable changes in Ae. speltoides were detected as decreases or losses in protein profiles. As a result of salt stress, all the remarkable changes in T. durum were detected as the increases in proteins. However, some proteins increased in T. aestivum, T. monococcum and Ae. tauschii. It is suggested that an increase in the amount of the proteins may lead to an increase in the tolerance mechanisms towards NaCl salinity of wheat species which has A and/or genome(s).

cDNA- AFLP analysis of the response of tetraploid black locust (Robinia pseudoacacia L.) to salt stress

“…But these studies will not be sufficient to explain the adaptation of polyploid plants, which may survive under severe salt stress. In practice, many polyploid plants exhibited higher tolerance to salt stress as compared with their diploid relatives (Saleh et al, 2008;Yıldız and Terzi, 2008;Mouhaya et al, 2010;Meng et al 2011). Therefore, polyploidy has been regarded as an efficient way to improve salt stress tolerance in plants and has played important roles in the practices of agriculture and forestry (Xiong et al, 2006).…”