Effects of nanosecond pulsed electric fields on the human prostate cancer cell line LNCaP

Donthula, V.; Camps-Raga, B.; Islam, Naz E.; Ślusarz, Anna; Lubahn, Dennis B.; Ganjam, Venkataseshu K.

doi:10.1109/tdei.2009.5293943

Cited by 5 publications

(3 citation statements)

References 75 publications

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“…Electric pulsed fields are known to induce a wide range of cellular responses, including reversible electroporation (EP, approximately in the range of 0.1–1 kV/cm and 100 µs) [Weaver, ], intracellular manipulation (IEM, approximately 10–1000 kV/cm and duration less than 100 ns) [Hair et al, ], and apoptosis induction with electric pulses (AIEP) or irreversible electrical breakdown (IREB, parameters between EP and IEM) [Al‐Sakere et al, ; Tang et al, ]. The parameters of the electric pulses applied in this work (10 kV/cm, 500 ns) lie in the range of IREB, and the effects of electric pulses on HepG2 cells were more similar to studies using pulses of microseconds and kilovolts per meter [Deng et al, ; Li et al, ], rather than those of nanosecond duration [Stacey et al, ; Donthula et al, ].…”

Section: Discussionmentioning

confidence: 99%

“…Ultrashort electric pulses have been shown to induce different physiological changes in cells including calcium bursts, caspase activation, phosphatidylserine (PS) externalization, growth inhibition in tumor cells, release of calcium from endoplasmic reticulum and mitochondria, and changes in DNA concentration [Beebe et al, ; Vernier et al, ; Nuccitelli et al, ; Donthula et al, ]. For cells subjected to external electric fields with an electric intensity higher than 10 kV/cm, longer pulses (>300 ns) always result in irreversible electroporation [Chen et al, ; Long et al, ; Ren and Beebe, ] while shorter electric pulses (<60 ns) can affect intracellular structures without disruption of the cell membrane [Schoenbach et al, ].…”

Section: Introductionmentioning

confidence: 99%

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Irreversible electroporation and apoptosis in human liver cancer cells induced by nanosecond electric pulses

Xiao

Yao

Liu

et al. 2013

Bioelectromagnetics

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The goal of this study was to assess the effect of nanosecond electric pulses on HepG2 human liver cancer cells. Electric pulses with a high strength of 10 kV/cm, duration of 500 ns and frequency of 1 Hz were applied to the cells. After delivery of electric pulses, apoptosis, intracellular calcium ion concentrations, transmembrane mitochondrial potentials, electropermeabilization and recovery from electropermeabilization in cells were investigated. The results showed that electric pulse treatment for 20 s and more could trigger apoptosis in cells. Real-time observation indicated an immediate increase in intracellular calcium ion concentration and a dramatic decrease in mitochondrial membrane potential in cells responding to electric pulses. In subsequent experiments, propidium iodide uptake in cells emerged after exposure to electric pulses, indicating electropermeabilization of the cell membrane. Furthermore, recovery from electropermeabilization was not observed even 4 h after the stimulation, demonstrating that irreversible electropermeabilization was induced by electric pulses. In conclusion, electric pulses with a high strength and nanosecond duration can damage cancer cells, accompanied by a series of intracellular changes, providing strong evidence for the application of electric pulses in cancer treatment.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Irreversible electroporation and apoptosis in human liver cancer cells induced by nanosecond electric pulses

Xiao

Yao

Liu

et al. 2013

Bioelectromagnetics

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“…The use of microsecond electric pulse in IRE for other urological tumors, such as kidney cancer (NCT01967407, NCT02828709, NCT02298608) and urinary bladder neoplasm (NCT02430623), is continually being tested in clinical trials. Further considering urological tumors, nanosecond electric pulses are studied only at the in vitro level [ 16 ].…”

Section: The Theoretical Background Of Epmentioning

confidence: 99%

Electroporation-Based Treatments in Urology

Szlasa

Saczko

Kulbacka

2020

Cancers

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The observation that an application of a pulsed electric field (PEF) resulted in an increased permeability of the cell membrane has led to the discovery of the phenomenon called electroporation (EP). Depending on the parameters of the electric current and cell features, electroporation can be either reversible or irreversible. The irreversible electroporation (IRE) found its use in urology as a non-thermal ablative method of prostate and renal cancer. As its mechanism is based on the permeabilization of cell membrane phospholipids, IRE (as well as other treatments based on EP) provides selectivity sparing extracellular proteins and matrix. Reversible EP enables the transfer of genes, drugs, and small exogenous proteins. In clinical practice, reversible EP can locally increase the uptake of cytotoxic drugs such as cisplatin and bleomycin. This approach is known as electrochemotherapy (ECT). Few in vivo and in vitro trials of ECT have been performed on urological cancers. EP provides the possibility of transmission of genes across the cell membrane. As the protocols of gene electrotransfer (GET) over the last few years have improved, EP has become a well-known technique for non-viral cell transfection. GET involves DNA transfection directly to the cancer or the host skin and muscle tissue. Among urological cancers, the GET of several plasmids encoding prostate cancer antigens has been investigated in clinical trials. This review brings into discussion the underlying mechanism of EP and an overview of the latest progress and development perspectives of EP-based treatments in urology.

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Pulsed Electric Fields for Food Preservation: An Update on Technological Progress

Nafchi¹,

Bhat²,

Alias³

2012

Progress in Food Preservation

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Effects of nanosecond pulsed electric fields on the human prostate cancer cell line LNCaP

Cited by 5 publications

References 75 publications

Irreversible electroporation and apoptosis in human liver cancer cells induced by nanosecond electric pulses

Irreversible electroporation and apoptosis in human liver cancer cells induced by nanosecond electric pulses

Electroporation-Based Treatments in Urology

Pulsed Electric Fields for Food Preservation: An Update on Technological Progress

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