Aspergillus niger is a common contaminant in food industry, laboratories, and also a potential threat to biological works of art in museums. Cotton textiles have frequently been used in museums for canvas paintings. In the present project, the effect of Aspergillus niger on fluid flow rate of nanowollastonite-impregnated cotton textile specimens was investigated. Cotton specimens were impregnated with nanowollastonite (NW) suspension at four concentrations of 10%, 20%, 30%, and 40% to be further compared with control specimens. Results showed that fluid flow in cotton textile was as high as 361.3 cm3·s−1 due to its high porous structure and very low compactness of fibers (low density). Impregnation with NW did not have a significant effect on fluid flow in cotton textile. Exposure to Aspergillus niger increased fluid flow in control specimens as a result of deterioration of cotton fibers. Exposure of NW-impregnated specimens at concentrations more than 20% to Aspergillus niger did not have any significant effect on fluid flow. In control specimens, fungus mycelium penetrated deep into the texture of textile. However, in NW-impregnated specimens, the fungus could not penetrate into the texture and deteriorate the specimens. It was concluded that NW can be recommended for textile industry and also works of art as they protect cotton textiles against Aspergillus niger while, do not diminishi its dying and paintability properties.