Effects of Native and Permethylated Cyclodextrins on the Catalytic Activity of L-Tryptophan indole lyase

The influence of selected O-alkyl derivatives of cyclodextrins on the enzymatic decomposition of l-tryptophan by l-tryptophan indole-lyase

“…In order to assess the relation between the values K 1 i , obtained in this work, to the geometry of formed complexes it is worth to compare these results with our previous data, 8 which are shown in Figure 9 as the graphical comparison of K 1 i values versus the type of selectively O-methylated a-and b-CDs. For the a-CD derivatives, the strongest competitive inhibition is observed for the native one, whereas the weakest inhibition of this type is observed for TM-a-CD.…”

“…8 It has been found that each of the studied CDs caused the inhibition of the enzymatic process. The observed inhibition has been of a mixed type, both competitive and non-competitive, in each case.…”

“…2 Therefore, an increased interest in applications of CDs in enzymology has been observed in the past few years. [3][4][5][6][7][8] The CDs are used in enzyme studies as additives 3 (which alter the rate of the enzymatic reactions) and also as covalent modifiers of the enzymes 4 (the modified enzyme was more catalytically active and thermally stable than the native one). Another example of the applications of modified CDs in enzymology has been the co-lyophilization of CDs with the enzymes.…”

A facile enantioseparation for amino acids enantiomers using β-cyclodextrins functionalized Fe3O4 nanospheres

“…When L-tryptophan interacts with the b-CD, the aromatic part of tryptophan inserts into the hydrophobic cavity of b-CD, however, the hydroxyl-terminated part of tryptophan would insert into the hydrophobic cavity of b-CD when D-tryptophan interacts with b-CD. 24 As shown in Fig. 1b, the signal for H-g is shifted upfield by about 0.015 ppm, and the signal for H-c is dramatically shifted upfield by about 0.45 ppm; both of which belong to the aromatic portion of tryptophan.…”