Effects of Naturally Occurring Antioxidants on Peroxidase Activity of Vegetable Extracts

Hemeda, H. M.; Klein, Barbara P.

doi:10.1111/j.1365-2621.1990.tb06048.x

Cited by 551 publications

(232 citation statements)

References 30 publications

Supporting

Mentioning

225

Contrasting

Unclassified

Order By: Relevance

“…PO activity was carried out, following the method of Hemeda and Klein (1990). The substrate was prepared with 5 ml of 1 % guaiacol, 5 ml of 0.3 % H 2 O 2 mixing and 50 ml of 0.05 M sodium phosphate buffer, pH 6.5.…”

Section: Peroxidase (Po)mentioning

confidence: 99%

Abiotic elicitors mediated elicitation of innate immunity in tomato: an ex vivo comparison

Chakraborty

Ghosh²,

Chandra

et al. 2016

Physiol Mol Biol Plants

View full text Add to dashboard Cite

Improvement of the host resistance by using hazard free chemical elicitors is emerging as an alternative approach in the field of plant disease management. In our present work, we have screened the efficacy and possible mechanism of abiogenic elicitors like Dipotassium hydrogen orthophosphate (K 2 HPO 4 ), Oxalic acid (OA), Isonicotinic acid (INA), Salicylic acid (SA), Acetylsalicylate (AS), Arachidonic acid (AA) and Calcium chloride (CaCl 2 ) to stimulate innate immune responses in Lycopersicum esculentum Mill. Excised tomato leaves, treated with elicitors at three different concentrations, were found to stimulate defense and antioxidative enzymes, total phenol and flavonoid content after 24 h of incubation. CaCl 2 (0.5 %) followed by INA (2.5 mM) were found most effective in activation of all such defense molecules in tomato leaves. Furthermore, nitric oxide (NO), a key gaseous mediator in plant defense signaling, was also measured after subsequent elicitor application. Higher doses of elicitors showed an elevated level of reactive oxygen species (ROS) generation, enhanced lipid peroxidation rate and proline content, which indicates the extent of abiotic stress generation on the leaves. However, ROS production, lipid peroxidation rate and proline concentration remain significantly reduced as a result of CaCl 2 (0.5 %) and INA (2.5 mM) application. A sharp increase of total chlorophyll content was also recorded due to treatment of CaCl 2 (0.5 %). These results demonstrate the effects of different abiogenic elicitors to regulate the production of defense molecules. Results also suggest that among all such chemicals, CaCl 2 (0.5 %) and INA (2.5 mM) can be used as a potential elicitor in organic farming of tomato.

show abstract

Section: Peroxidase (Po)mentioning

confidence: 99%

Abiotic elicitors mediated elicitation of innate immunity in tomato: an ex vivo comparison

Chakraborty

Ghosh²,

Chandra

et al. 2016

Physiol Mol Biol Plants

View full text Add to dashboard Cite

show abstract

“…The definition of one unit of SOD was the enzyme amount causing 50% inhibition of the nitroblue tetrazolium reduction rate. The activity of POD was measured according to Hemeda and Klein [35]. The activity of POD was calculated using the extinction coefficient of 2.66 Â 10 4 M À1 cm À1 at a wavelength of 470 nm and a stoichiometry of 4.…”

Section: Assay Of Reduced Gsh Content and Antioxidant Enzyme Activitiesmentioning

confidence: 99%

Combined toxicity of polycyclic aromatic hydrocarbons and heavy metals to biochemical and antioxidant responses of free and immobilized Selenastrum capricornutum

Wang¹,

Luo²,

Lin³

et al. 2012

Environmental Toxicology and Chemistry

View full text Add to dashboard Cite

Abstract-The aquatic environment often contains different groups of contaminants, but their combined toxicity on microalgae has seldom been reported. The present study compared the toxic effects of combined mixed polycyclic aromatic hydrocarbons (PAHs) and heavy metals on growth and antioxidant responses of free and immobilized microalga, Selenastrum capricornutum. Five PAHsphenanthrene, fluorene, fluoranthene, pyrene, and benzo[a]pyrene-and four heavy metals at different concentrations-0.05 to 0.1 mg Cd 2þ ml À1 , 0.05 to 1 mg Cu 2þ ml À1 , 0.05 to 1 mg Zn 2þ ml À1 , and 0.5 to 2.5 mg Ni 2þ ml À1 -were examined. Results showed that the chlorophyll a content of free and immobilized S. capricornutum was not affected by PAHs but was significantly inhibited by heavy metals. Conversely, the antioxidant parameters, including the content of reduced glutathione (GSH) and the activities of superoxide dismutase and peroxidase, were significantly induced by both PAHs and metals. For the combined toxic effects of PAHs and heavy metals, cell growth and antioxidant responses varied with exposure time and contaminants and differed between free and immobilized cells. The effects of cocontaminants on the GSH content in free cells were mainly synergistic but changed to antagonistic in immobilized cells. The toxic effects of cocontamination on free cells were also more obvious than those on immobilized cells. These findings suggest that immobilization offers some protection to microalgal cells against toxic contaminants causing differences in the interaction and responses to combined toxicants between free and immobilized cells. Immobilized cells might be more suitable for treating wastewater containing toxic contaminants than free cells. Environ. Toxicol. Chem. 2013;32:673-683. # 2012 SETAC

show abstract

“…Guaiacol peroxidase (GPX; EC 1.11.1.7) was determined using 2 ml of reaction mixture consisting of 0.1 mM phosphate buffer (pH 6.0), 45 mM guaiacol (v/v), 44 mM H 2 O 2 and a suitable aliquot of enzyme extract. Oxidation of guaiacol (extinction coefficient = 26.6 mM -1 cm -1 ) was calculated at 470 nm and activity was defined as moles of guaiacol oxidized per minute per gram fresh weight 11 . Glutathione reductase (GR; EC 1.6.4.2) activity was measured using 1 ml of reaction mixture containing 0.1 M potassium phosphate buffer (pH 7.8), 1 mM EDTA, 0.2 mM NADPH, 0.5 mM GSSG and a suitable aliquot of enzyme extract using extinction coefficient of…”

Section: Estimation Of Antioxidant Enzymesmentioning

confidence: 99%

Interactive Effects of EDTA and Oxalic Acid on Chromium Uptake, Translocation and Photosynthetic Attributes in Indian Mustard (<i>Brassica juncea</i> L. var. Varuna)

2017

View full text Add to dashboard Cite

The accumulation and toxicity of chromium (Cr) in Indian mustard (Brassica juncea L.) under ethylene diamine tetraacetic acid (EDTA) and oxalic acid (OA) as chelating agents was studied. Plants were exposed to Cr(VI) and chelating agents in four experimental set-ups as Cr(VI), Cr(VI) + EDTA (1 : 1), Cr(VI) + OA (1 : 1) and Cr(VI) + EDTA + OA (1 : 1 : 1), where each set-up comprised of 0, 6.25, 12.5 and 25.0 mg of Cr(VI) and/or chelating agents in 250 g of soil. Results conferred that EDTA augmented bio-concentration factor in all the three doses of Cr(VI), OA considerably increased translocation factor in all the treatments, including control, and the combined application of the two chelates escalated both the aforesaid factors. Moreover, these chelating agents help in ameliorating Cr(VI) toxicity asserted by low degree of lipid peroxidation, insubstantial damage in root and shoot length, fresh and dry biomass, chlorophyll and leaf gas exchange parameters. Besides, plants showed a robust detoxification mechanism primarily by significant (P < 0.05) production of reduced glutathione and phytochelatins among other enzymatic and nonenzymatic antioxidants under these chelating agents. The present findings suggest that Indian mustard could be used as a potential phytoremediator of Cr(VI) under the combined application of EDTA and OA.

show abstract

Effects of Naturally Occurring Antioxidants on Peroxidase Activity of Vegetable Extracts

Cited by 551 publications

References 30 publications

Abiotic elicitors mediated elicitation of innate immunity in tomato: an ex vivo comparison

Abiotic elicitors mediated elicitation of innate immunity in tomato: an ex vivo comparison

Combined toxicity of polycyclic aromatic hydrocarbons and heavy metals to biochemical and antioxidant responses of free and immobilized Selenastrum capricornutum

Interactive Effects of EDTA and Oxalic Acid on Chromium Uptake, Translocation and Photosynthetic Attributes in Indian Mustard (<i>Brassica juncea</i> L. var. Varuna)

Contact Info

Product

Resources

About