Effects of neem oil (Azadirachta indica A. Juss) on the replacement of the midgut epithelium in the lacewing Ceraeochrysa claveri during larval-pupal metamorphosis

Scudeler, Elton Luiz; Padovani, Carlos Roberto; Santos, Daniela Carvalho dos

doi:10.1016/j.acthis.2014.01.008

Cited by 29 publications

(22 citation statements)

References 66 publications

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“…The emergence of these structures are evidence of cellular degeneration (Scudeler and Santos, 2013). Similar results were found by Ghribi et al (2012) studying insects treated with biosurfactantss and by Scudeler et al (2014) in insects treated with neem oil (Azadirachta indica). As the generative cells are responsible for the production of new digestive cells for the digestion process (Agbede and Kemp, 1985;Agyei and Runham, 1995;Harrison and Foelix, 1999;Walker and Fletcher, 1987) and here these cells showed evidence of cell death after the treatment, the mitotic divisions would probably not be occurring, or would be deficiently occurring, inhibiting the adequate replacement of the cells released from the basal membrane during digestion and of the worn-out digestive cells.…”

Section: Discussionsupporting

confidence: 59%

Dinotefuran-induced morphophysiological changes in semi-engorged females Rhipicephalus sanguineus Latreille, 1806 (Acari: Ixodidae) ticks: Ultra-structural evaluation

et al. 2017

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The present study demonstrated the effects of dinotefuran (active ingredient of the acaricide Protetor Pet) on the ovary and midgut cells of semi engorged R. sanguineus females exposed to different concentrations of this chemical. For this, 120 semi-engorged females were divided into four treatment groups with 30 individuals each: group I or control (distilled water), group II (5000ppm), groups III (6250ppm) and group IV (8334ppm of dinotefuran). All the ticks were immersed in the different concentrations of dinotefuran or in distilled water for 5min and then dried and kept in BOD incubator for 7days. The results showed alterations mainly regarding the damaged cell structures, such as yolk granules, organelles and the plasma membrane of the germ cells. In addition, structures related with defense mechanisms were found, such as vacuoles, cytoskeletal filaments, and myelin figures in the germ cells. Damages in the generative cells of the midgut, alterations in the size of digestive cells, the number of endosomes, digestive vacuoles, digestive residues, lipid drops and organelles in the cytoplasm of the digestive cells and the presence of microvilli in the plasma membrane of these cells also demonstrate the progressive damages caused by the action of dinotefuran in the midgut and germ cells of R. sanguineus semi-engorged females. The concentrations applied partially impaired the digestive processes; and, without proper nutrition, all the ectoparasite's physiologic events are prevented from occurring, leading the individual to death. The germ cells were also damaged, and probably would not be able to advance in their development (I-V) and complete the vitellogenesis, which would affect the fertility of the female and consequently impede the formation of a new individual.

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Section: Discussionsupporting

confidence: 59%

Dinotefuran-induced morphophysiological changes in semi-engorged females Rhipicephalus sanguineus Latreille, 1806 (Acari: Ixodidae) ticks: Ultra-structural evaluation

et al. 2017

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“…In this work, we observed the same changes caused by anacardic acid, a phenolic lipid that constitutes the most of the liquid from the bark of the cashew nut. The changes described in this work are mainly caused due to a loss of control of the ionic balance and water intake that is commonly reported in treatments with oils, plant extracts and toxins (Scudeler et al, 2014). The loss of the cytoplasm due to the release of cytoplasmic protrusions may be indicative of a toxic effect of anacardic acid in the columnar cells.…”

Section: Histological Analysesmentioning

confidence: 79%

“…The epithelial cells showed stratified, irregular nucleus and vacuoles. Scudeler et al (2014) define that the stratification and transformation of a simple columnar epithelium into a stratified epithelium, that is, new replacement of cell layers showed that a sophisticated defense may occur to replace the loss as a result of local lesions induced by cells acid. Costa et al (2012), in studies on A. aegypti larvae exposed with methanolic extract of Annona coriacea (Magnoliales: Annonaceae), described serious histopathological changes, such as vacuolization of the cytoplasm, hypertrophy of epithelial cells and their nucleus, deterioration of the brush border, cell disintegration and apical vesicles formation that released their material in the intestinal lumen.…”

Section: Histological Analysesmentioning

confidence: 99%

Effects of cashew nut shell liquid (CNSL) component upon Aedes aegypti Lin. (Diptera: Culicidae) larvaes midgut

Dourado¹,

Rosa²,

Porto³

et al. 2015

Afr. J. Biotechnol.

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The cashew nut shell liquid (CNSL) has been associated with a number of biological activities. The aim of this study was to evaluate the insecticidal potential caused by CNSL from Anacardium occidentalle L. (Anacardiaceae) upon Aedes aegypti and verify histomorphological alterations in the larval midgut. The experiments were carried out using third instar A. aegypti larvae, exposed to CNSL at different concentrations. After 24 h, the larvae were treated and stained with hematoxylin-eosin (HE). Morphometric analyzes were performed on the A. aegypti larvae midgut and registered by photomicroscopy. Anacardic acid was identified in CNSL by high performance liquid chromatography (HPLC) and showed 69% purity. The minimum concentration of CNSL that promoted mortality of A. aegypti larvae (LC 10) was 0.01 mg mL-1 ; the LC 90 was 0.139 mg mL-1 and the LC 50 was 0.07 mg mL-1. Changes in the midgut were severe in larvae treated with CNSL, especially at concentrations of 1.0 to 0.01 mg mL-1 ; degeneration of the lining, hypersecretion of epithelial cells, increased vacuoles, and separation of the epithelial cells from the basal membrane, and disintegration of the brush border and damage of the peritrophic membrane occurred. CNSL caused damage to the midgut of 3 rd instars of A. aegypti by irreversibly disrupting their complete larval development.

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“…In advanced stages, that Bunder nutrition^will lead the ectoparasite to death. Other studies by Scudeler et al (2014) also detected the appearance of the histochemical alterations in the midgut of C. claveri larvae treated with neem oil.…”

Section: Discussionmentioning

confidence: 79%

Dinotefuran-induced morphophysiological changes in the ovaries and midgut of semi-engorged females Rhipicephalus sanguineus Latreille, 1806 (Acari: Ixodidae) ticks

et al. 2015

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The present study demonstrated the effects of dinotefuran (active compound of the Protetor Pet® acaricide) in germ cells and the digestive processes of semi-engorged females of R. sanguineus exposed to different concentrations of the chemical. For this purpose, 120 semi-engorged females were divided into four treatment groups with 30 individuals each: group I or control (distilled water), group II (5000 ppm), group III (6250 ppm), and group IV (8334 ppm of dinotefuran). All ticks were immersed in different concentrations of dinotefuran or in distilled water for 5 min and then were dried and stored in biological oxygen demand (BOD) incubator for 7 days. The results show the action of this compound, exhibiting morphohistologic and histochemical changes in the oocytes and the midgut cells of individuals of different groups, which were compared with those of group I (control). The alterations occurred mainly in relation to the size of the germ cells and yolk granules; presence, quantity, size, and location of vacuoles found in the cytoplasm of these germ cells; the damage occurred in the generative cells of the midgut; the size of the digestive cells; the quantity of blood elements captured, accumulated digestive wastes and digestive vacuoles found in the cytoplasm of the digestive cells of the midgut, as well as the amount and distribution of proteins, polysaccharides, lipids of all cells in both organs. So, it has demonstrated the effectiveness of dinotefuran in the reduction of fertility and digestive processes of semi-engorged females of R. sanguineus, data that points the possibility of employing this chemical to control these ectoparasites.

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Effects of neem oil (Azadirachta indica A. Juss) on the replacement of the midgut epithelium in the lacewing Ceraeochrysa claveri during larval-pupal metamorphosis

Cited by 29 publications

References 66 publications

Dinotefuran-induced morphophysiological changes in semi-engorged females Rhipicephalus sanguineus Latreille, 1806 (Acari: Ixodidae) ticks: Ultra-structural evaluation

Dinotefuran-induced morphophysiological changes in semi-engorged females Rhipicephalus sanguineus Latreille, 1806 (Acari: Ixodidae) ticks: Ultra-structural evaluation

Effects of cashew nut shell liquid (CNSL) component upon Aedes aegypti Lin. (Diptera: Culicidae) larvaes midgut

Dinotefuran-induced morphophysiological changes in the ovaries and midgut of semi-engorged females Rhipicephalus sanguineus Latreille, 1806 (Acari: Ixodidae) ticks

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