Effects of Nelumbo nucifera Gaertn. Petal Tea Extract on Hepatotoxicity and Oxidative Stress Induced by Mancozeb in Rat Model

Abstract

Mancozeb (Mz) is one of the most widely used pesticides that has been reported to cause adverse human health risks. White Nelumbo nucifera (N. nucifera) petals have therapeutic properties to prevent toxicity. Hence, this study attempted to determine the effects of N. nucifera extract on hepatotoxicity and oxidative stress in mancozeb-treated rats. Seventy-two male rats were divided into nine groups and designed with a control; N. nucifera extract was administered at the doses of 0.55, 1.1, and 2.2 mg/kg bw/day, Mz was administered at 500 mg/kg bw/day, and the co-treatment groups (N. nucifera and Mz) were administered 0.55, 1.1, and 2.2 mg/kg bw/day of N. nucifera followed by administering Mz 500 mg/kg bw/day daily for 30 days. The results showed that all doses of N. nucifera extract did not induce hepatic toxicity and could suppress the toxicity of mancozeb by increasing body weight gain and decreasing relative liver weight, lobular inflammation, and total injury score. The combination treatment also decreased the molecular markers of oxidative stress (2-hydroxybutyric acid, 4-hydroxynonenal, l-tyrosine, pentosidine, and N6-carboxymethyllysine). Furthermore, the reduced glutathione and oxidized glutathione contents were adjusted close to the normal level. Therefore, N. nucifera extract is a natural antioxidant supplement that could decrease the toxicity of mancozeb and can be safely consumed.

Neuroprotective Effect of White Nelumbo nucifera Gaertn. Petal Tea in Rats Poisoned with Mancozeb

“…In short, mancozeb has been shown to induce relative brain weight increase in acute and subchronic periods and decrease relative brain weight at high doses in chronic time scales. Similarly, a previous studies showed increased relative brain and liver weights in albino rats poisoned with mancozeb at a dose of 500 mg/kg bw for 30 days due to hypertrophy of both tissues [50,51]. Moreover, subchronic treatment with chlorpyrifos-methyl, a type of pesticide, also caused an increase in relative brain weight due to minor histopathological changes in the brain [52].…”

“…Our present study is similar to an in vivo study of the effect of white N. nucifera petal extract on Charolais cattle sperm exposed to mancozeb at low doses of 0.22 and 0.44 µg/mL, which showed no significant differences in the AOPPs and AGEs [12]. Furthermore, a prior study showed white N. nucifera petal extract decreased the levels of oxidative stress markers (2-hydroxybutyric acid, 4-hydroxynonenal, l-tyrosine, pentosidine, and N6-carboxymethyllysine) on rat livers poisoned with mancozeb [50].…”

The dithiocarbamate pesticides maneb and mancozeb disturb the metabolism of lipids and xenobiotics in an in vitro model of metabolic dysfunction-associated steatotic liver disease

“…Zn supplementation in individuals exposed to high levels of dithiocarbamates might be an efficient means to prevent their potential hepatotoxicity. Although to the best of our knowledge there are no clinical or epidemiological studies reporting dithiocarbamateinduced liver injury, maneb and mancozeb can cause hepatic damage in rodents in particular via oxidative stress and DNA damage (Aprioku et al 2023;Ben Amara et al 2015;Nuchniyom et al 2023;Saber et al 2019;Sefi et al 2019;Zhang et al 2022b). Interestingly, Zn supplementation was reported to prevent maneb-induced teratogenicity in rats (Larsson et al 1976) and maneb-induced kidney injury in mice (Sefi et al 2020), thus suggesting that Zn could prevent dithiocarbamate toxicity in different tissues and organs.…”