Effects of Neospora caninum Infection at Mid-Gestation on Placenta in a Pregnant Mouse Model

López-Pérez, Inmaculada C.; Collantes-Fernández, Esther; Rojo-Montejo, Silvia; Navarro-Lozano, Vanesa; Risco-Castillo, Verónica; Pérez-Pérez, V.; Pereira-Bueno, J.; Ortega‐Mora, Luis Miguel

doi:10.1645/ge-2347.1

Cited by 17 publications

(15 citation statements)

References 29 publications

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“…Previously, a high parasite burden was observed in the placentas from dams infected at mid-gestation, suggesting that N. caninum primarily multiplies in the placenta during the initial stages of infection. Moreover, IL-4 was upregulated in placentas which may enhance N. caninum susceptibility at the materno-foetal interface and favour transmission to the progeny (López-Pérez et al 2010). In addition, we also previously reported that infection at the three pregnancy periods produced different foetal and neonatal mortality and transmission rates to the offspring (López-Pérez et al 2006.…”

Section: Discussionmentioning

confidence: 71%

“…In this model, we detected an increase in IL-4 expression in placentas from mice infected at mid-gestation, which might favour the multiplication of the parasite and facilitate its transmission to the foetus across the placenta. Furthermore, during the initial infection, when the parasite is mainly multiplying in the placenta, we observed an increase in TNF-α expression in N. caninum PCR-positive placentas, which could be associated with embryotoxic effects (López-Pérez et al 2010).…”

Section: Introductionmentioning

confidence: 72%

“…Synthesis of cDNA was performed with SuperScript II Reverse Transcriptase (Invitrogen), according to the manufacturer's recommendations. The primer sequences used in this study for cDNA amplification of IFN-γ, IL-10, IL-4, and β-actin were previously published (Varona et al 2005, López-Pérez et al 2010. β-actin was employed as an endogenous reference for each corresponding sample.…”

Section: Total Rna Extraction and Real-time Rt-pcrmentioning

confidence: 99%

“…Mice inoculated at early gestation show an increase in resorptions and a high vertical transmission rate; in contrast, no foetal losses and low transmission rates are detected when dams are infected late in gestation (Long and Baszler 1996;Liddell et al 1999;Quinn et al 2002b;López-Pérez et al 2006. We previously developed a pregnant mouse model to further explore the pathogenesis of N. caninum infection during gestation (López-Pérez et al 2006, 2010. In this model, we detected an increase in IL-4 expression in placentas from mice infected at mid-gestation, which might favour the multiplication of the parasite and facilitate its transmission to the foetus across the placenta.…”

Section: Introductionmentioning

confidence: 99%

“…Neospora caninum in mice at early and late gestation 253 Pérez et al 2010) could explain why infection at this stage provoked greater foetus damage than infection in early or late gestation, leading to the highest mortality and vertical transmission rates along time (López-Pérez et al 2006.…”

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confidence: 99%

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Pathological and immunological findings in placentas from pregnant BALB/c mice infected with Neospora caninum at early and late stages of gestation

López-Pérez

Collantes-Fernández

Rojo-Montejo

et al. 2011

Acta Parasitologica

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Neospora caninum is transmitted from a cow to its foetus by vertical transmission and the timing of infection in gestation is an important factor in determining the disease outcome. Few studies have explored the role of the placenta in the outcome of N. caninum infection during pregnancy. Here, we described the N. caninum presence, parasite load, local immune response, and histopathological lesions at the materno-foetal interface after infection of BALB/c mice at early and late stages of gestation. In mice infected at early gestation, N. caninum DNA was detected in foetoplacentary units 7 days post-infection (PI) and in the placenta, but not in viable foetuses on day 14 PI, indicating that the parasite was multiplying primarily in the placental tissues without reaching the foetus. Moreover, parasite DNA was detected in resorptions, suggesting that foetal death could be a consequence of infection. An increase in IFN-γ, TNF-α and IL-10 expression was observed in N. caninum PCR-positive placentas, which could favour N. caninum foetal transmission and be harmful to both the placenta and the foetus. Histopathological analysis revealed necrosis affecting both the maternal and foetal sides of the placenta. At late gestation, transmission occurred rapidly following infection (day 3 PI), but parasite were rarely found. In addition, an increase in cytokine expression was observed in spleen and placental tissues from infected animals, while a downregulation in IL-4 expression was only observed in the spleen. Finally, necrosis in the placenta was limited to the maternal side, suggesting that the parasite is mainly multiplying in the placental tissue at this stage. Thus, the results of the present study indicate that the placenta may be actively involved in N. caninum pathogenesis.

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Section: Discussionmentioning

confidence: 71%

Section: Introductionmentioning

confidence: 72%

Section: Total Rna Extraction and Real-time Rt-pcrmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Pathological and immunological findings in placentas from pregnant BALB/c mice infected with Neospora caninum at early and late stages of gestation

López-Pérez

Collantes-Fernández

Rojo-Montejo

et al. 2011

Acta Parasitologica

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Activities of 11‐Azaartemisinin andN‐Sulfonyl Derivatives againstNeospora caninumand Comparative Cytotoxicities

et al. 2017

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Neosporosis caused by the apicomplexan parasite Neospora caninum is an economically important disease that induces abortion in dairy and beef cattle. There are no vaccines or drugs available on the market for control or treatment of the disease in bovines. The peroxide artemisinin and its derivatives used clinically for treatment of malaria are active against N. caninum and other apicomplexan parasites. We have now evaluated the activities of the readily accessible and chemically robust 11-azaartemisinin 5 and selected N-sulfonyl derivatives prepared as described in the accompanying paper against N. caninum tachyzoites grown in infected human foreskin fibroblasts. Azaartemisinin elicited an IC value of 150 nm, and the 2',5'-dichloro-3'-thienylsulfonyl-11-azaartemisinin 17 was found to be the most active, with an IC value of 40 nm. Comparison with normal human fetal lung fibroblasts HFLF WI-38 revealed relatively benign cytotoxicity. The compounds were also screened in vitro against TK-10 (renal), UACC-62 (melanoma) and MCF-7 (breast) cancer cell lines; overall, in line with activities against HFLF cells, most compounds in the series were found to be inactive.

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Establishment of a model of Neospora caninum infection in pregnant mice

Jia

Xie

et al. 2020

Parasitol Res

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Effects of Neospora caninum Infection at Mid-Gestation on Placenta in a Pregnant Mouse Model

Cited by 17 publications

References 29 publications

Pathological and immunological findings in placentas from pregnant BALB/c mice infected with Neospora caninum at early and late stages of gestation

Pathological and immunological findings in placentas from pregnant BALB/c mice infected with Neospora caninum at early and late stages of gestation

Activities of 11‐Azaartemisinin andN‐Sulfonyl Derivatives againstNeospora caninumand Comparative Cytotoxicities

Establishment of a model of Neospora caninum infection in pregnant mice

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