Effects of nerve growth factor on the survival and synaptic function of Ia sensory neurons axotomized in neonatal rats

Miyata, Yuhei; Kashihara, Yasuhiro; Homma, Shinji; Kuno, Motoý

doi:10.1523/jneurosci.06-07-02012.1986

Cited by 120 publications

(72 citation statements)

References 29 publications

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“…and Methods Surgical procedures were exactly the same as those described in the previous paper (Miyata et al, 1986). Wistar rats were anesthetized with ether the day after birth, and the nerve to the medial gastrocnemius (MG) muscle was crushed with a Dumont forceps at the bifurcation point near its entry to the muscle in the left hind leg.…”

Section: Methodsmentioning

confidence: 99%

“…The preceding study (Miyata et al, 1986; also see Kuno et al, 1985) showed that monosynaptic excitatory postsynaptic potentials (EPSPs) evoked in spinal motoneurons by muscle afferent volleys are strikingly depressed following crush of the muscle nerve in neonatal rats and that this synaptic depression is partially reversed by daily treatment with NGF. Thus, the central synaptic function of Group Ia sensory fibers in neonatal animals appears to be influenced by exogenous NGF under certain conditions.…”

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confidence: 99%

“…It is known that crush of the peripheral nerve in neonatal rats results in massive cell death of the sensory neurons (Bondok and Sansone, 1984;. However, NGF treatment does not prevent the cell death of large sensory neurons (Miyata et al, 1986). Therefore, there is little doubt that central terminals of Group Ia fibers undergo some alterations in response to NGF, either increasing transmitter release or producing sprouting.…”

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confidence: 99%

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Effects of nerve growth factor on differentiation of muscle spindles following nerve lesion in neonatal rats

Sekiya¹,

Homma²,

Miyata³

et al. 1986

J. Neurosci.

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Central synaptic function of Group Ia sensory fibers has been shown to be enhanced by daily applications of NGF in neonatal rats. In the periphery, Group Ia sensory fibers are known to trigger the differentiation of muscle spindles. In the present study we examined, in neonatal rats, whether daily NGF treatment affects the number or structure of spindles formed in muscle whose nerve has been crushed. The results show that 25-35 d after nerve crush, de uwo supernumerary spindles were formed by treatment with NGF in the temporarily denervated muscle.

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Effects of nerve growth factor on differentiation of muscle spindles following nerve lesion in neonatal rats

Sekiya¹,

Homma²,

Miyata³

et al. 1986

J. Neurosci.

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“…Similarly. Miyata et al (1986) had reported a significant increase in the loss of motoneuron cell bodies after crush lesion of the medial gastrocnemius nerve of newborn rats after treatment with NGF (289c death after lesion in the absence of NGF, increased to 429c in NGF treated animals). At the concentration of NGF with which we obserye a reduction in BDNF-mediated motoneuron survival in vitro.…”

Section: Effects Of Combinations Of Survival Factors Formentioning

confidence: 99%

Members of several gene families influence survival of rat motoneurons in vitro and in vivo

Hughes¹,

Sendtner²,

Thoenen³

1993

J of Neuroscience Research

276

179

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The survival and functional maintenance of spinal motoneurons, both during the period of developmental cell death and in adulthood, have been shown to be dependent on trophic factors. In vitro experiments have previously been used to identify several survival factors for motoneurons, including CNTF, UF, and members of the neurotrophin, FGF, and IGF gene families. Some of these factors have also been shown to be active in vivo, either on chick motoneurons during embryonic development or on lesioned facial and spinal motoneurons of the newborn rat. Here we demonstrate that lesioned newborn rat facial motoneurons can be rescued by NT-4/5, IGF-I, and UF. Furthermore, in contrast to chick motoneurons, the survival of isolated embryonic rat motoneurons can be maintained by the neurotrophins BDNF, NT-3. and NT-4/5. IGF-I and FGF-5 were also active in this system, each supporting more than 50% of the originally plated neurons. The responsiveness of motoneurons to multiple factors in vitro and in vivo suggests that motoneuron survival and function are regulated by the coordinated actions of members of different gene families.

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“…Spinal neural circuits undergo continuous functional and structural changes in response to a peripheral nerve injury (2). Some recent studies show that glutamate receptors may be involved in sensory and motor neuron apoptosis following severe nerve injury in rats (14,17,25,50). Glutamate is an important excitatory neurotransmitter in the nervous system.…”

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confidence: 99%

The effect of memantine on functional recovery of the sciatic nerve crush injury in rat

Ghayour

Abdolmaleki²,

Rassouli³

2016

Turkish Neurosurgery

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ABSTRACTin turn, triggers free radical formation and multiple pathways leading to the initiation of apoptotic-like damage (1,5). Therefore, glutamate excitotoxicity is an attractive therapeutic target for attenuation of neural tissue damage (13,32). Hence, NMDA receptor antagonists could potentially provide neuroprotective effects in several neurodegenerative diseases manifesting excessive stimulation of NMDA receptors, including stroke, nerve injury and neuropathic pain syndromes (2,21). However, attempts to use potent competitive NMDA receptor antagonists as neuroprotectants have shown serious side effects in patients (42).Memantine is a non-competitive N-methyl-d-aspartate glutamate (NMDA) receptor antagonist and is capable of blocking excitotoxicity (12). This drug only reduces excessive NMDA receptor activation when it is pathologically activated for long █ INTRODUCTION P eripheral nerves injuries result in loss of motor, sensory and autonomic function of the affected nerves. Spinal neural circuits undergo continuous functional and structural changes in response to a peripheral nerve injury (2). Some recent studies show that glutamate receptors may be involved in sensory and motor neuron apoptosis following severe nerve injury in rats (14,17,25,50). Glutamate is an important excitatory neurotransmitter in the nervous system. Neural trauma triggers massive release of glutamate from injured cells (2). Overactivation of the N-methyl-Daspartate (NMDA) glutamate receptor subtype can result in a process called excitotoxicity that leads to cell death (20,32). Excitotoxicity is due to excessive calcium ion influx which, AIm: Following severe peripheral nerve injury (PNI), regeneration is often insufficient and functional recovery is incomplete. In this regard, glutamate N-methyl-D-aspartate (NMDA) receptor antagonist such as Memantine have been shown to have neuroprotective effects. We evaluated the effects of Memantine against sciatic nerve crush injury in male Wistar Rats. mATERIAl and mEThODS: Memantine or vehicle was given parenteraly to rats for 7 days postoperative. In Memantine treatment groups, a single dose of agent (5 and 10 mg/kg) was administered daily. The control group was given vehicle in the same manner. The rats were subjected to crush injury in the left sciatic nerve with non-serrated clamp for 30 seconds. Behavioural, electrophysiological and morphological alterations were evaluated during the experimental period. RESUlTS:Results showed that Memantine has no significant effect on regeneration process rate and functional recovery quality. In the sciatic functional index (SFI) test no significant difference was observed between Memantine treatment groups (5 and 10 mg/ kg) at any week. CONClUSION:Since the major neuroprotective effect of Memantine is due to its protective activity against NMDA receptormediated excitotoxicity, it seems that glutamate excitotoxicity is less important in motor impairment due to sciatic nerve crush injury. It is clear that more research is needed to confirm these findin...

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Effects of nerve growth factor on the survival and synaptic function of Ia sensory neurons axotomized in neonatal rats

Cited by 120 publications

References 29 publications

Effects of nerve growth factor on differentiation of muscle spindles following nerve lesion in neonatal rats

Effects of nerve growth factor on differentiation of muscle spindles following nerve lesion in neonatal rats

Members of several gene families influence survival of rat motoneurons in vitro and in vivo

The effect of memantine on functional recovery of the sciatic nerve crush injury in rat

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