Effects of neuropeptides B and W on the rat pituitary-adrenocortical axis: In vivo and in vitro studies

Hochol, Anna; Tortorella, Cinzia; Ricinski, Marcin; Ziółkowska, Agnieszka; Nussdorfer, Gastone G.; Malendowicz, Ludwik K.

doi:10.3892/ijmm.19.2.207

Cited by 12 publications

(16 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…NPB and NPW were shown to pharmacologically stimulate adrenal glucocorticoid secretion by an ACTH-independent mechanism. It was also reported that NPW stimulates in vitro aldosterone secretion by enhancing the release of medullary catecholamines, which activate beta-adrenoceptors located on zona glomerulosa cells (Hochol et al, 2007). …”

Section: Biological Activities Of Npb and Npwmentioning

confidence: 99%

See 1 more Smart Citation

NPBWR1 and NPBWR2: Implications in Energy Homeostasis, Pain, and Emotion

Sakurai¹

2013

Front. Endocrinol.

View full text Add to dashboard Cite

Neuropeptide B/W receptor-1 (NPBWR1) and NPBWR2 had been known as orphan receptors GPR7 and 8, respectively. Endogenous peptide ligands of these receptors, neuropeptide B (NPB) and neuropeptide W (NPW), were identified in 2002 and 2003 (Fujii et al., 2002; Brezillon et al., 2003; Tanaka et al., 2003). These peptides have been implicated in regulation of feeding behavior, energy homeostasis, neuroendocrine function, and modulating inflammatory pain. In addition, strong and discrete expression of their receptors in the extended amygdala and bed nucleus of the stria terminalis suggests a potential role in regulating stress responses, emotion, anxiety, and fear. Recent studies of NPB/NPW using both pharmacological and phenotypic analyses of genetically engineered mice as well as a human study support this hypothesis.

show abstract

Section: Biological Activities Of Npb and Npwmentioning

confidence: 99%

“…Expression of Npb , Npw , and Npbwr1 mRNA in both the adrenal cortex and adrenal medulla has been reported (Andreis et al, 2005; Mazzocchi et al, 2005; Hochol et al, 2007). NPB and NPW were shown to pharmacologically stimulate adrenal glucocorticoid secretion by an ACTH-independent mechanism.…”

Section: Biological Activities Of Npb and Npwmentioning

confidence: 99%

NPBWR1 and NPBWR2: Implications in Energy Homeostasis, Pain, and Emotion

Sakurai¹

2013

Front. Endocrinol.

View full text Add to dashboard Cite

show abstract

“…It should also be noted that galanin is involved in the central control of feeding (2,3), and that other peptides playing a similar role (e.g. neuropeptide-Y, neuropeptide-W, leptin, orexins and cholecystokinins) are able to control glucocorticoid secretion acting on both the central and the peripheral branch of the hypothalamic-pituitary-adrenal axis (34)(35)(36)(37)(38). In addition, the possible adrenocortical secretagogue action of another preprogalanin-derived peptide, the galanin-like peptide (GALP), should be investigated.…”

Section: Discussionmentioning

confidence: 99%

Galanin stimulates cortisol secretion from human adrenocortical cells through the activation of galanin receptor subtype 1 coupled to the adenylate cyclase-dependent signaling cascade

Belloni

Malendowicz²,

Ruciński³

et al. 2007

Int J Mol Med

Self Cite

View full text Add to dashboard Cite

Abstract. Previous studies showed that galanin receptors are expressed in the rat adrenal, and galanin modulates glucocorticoid secretion in this species. Hence, we investigated the expression of the various galanin receptor subtypes (GAL-R1, GAL-R2 and GAL-R3) in the human adrenocortical cells, and the possible involvement of galanin in the control of cortisol secretion. Reverse transcription-polymerase chain reaction detected the expression of GAL-R1 (but not GAL-R2 and GAL-R3) in the inner zones of the human adrenal cortex. The galanin concentration dependently enhanced basal, but not ACTH-stimulated secretion of cortisol from dispersed inner adrenocortical cells (maximal effective concentration, 10 -8 M). The cortisol response to 10 -8 M galanin was abrogated by GAL-R1 immunoneutralization, and unaffected by GAL-R2 or GAL-R3 immunoneutralization. Galanin (10 -8 M) and ACTH (10 -9 M) enhanced cyclic-AMP production from dispersed cells, and the response was suppressed by the adenylate cyclase inhibitor SQ-22536 (10 -4 M). Galanin did not affect inositol triphosphate release, which, in contrast, was raised by angiotensin-II (10 -8 M). SQ-22536 and the protein kinase (PK)A inhibitor H-89 (10 -5 M) abolished the cortisol response to 10 -8 M galanin, while the phospholipase C inhibitor U-73122 and the PKC inhibitor calphostin-C were ineffective. Preincubation with pertussis toxin (Ptx) (0.5 μg/ml) partially inhibited the cortisol response to galanin. We conclude that galanin stimulates cortisol secretion from human inner adrenocortical cells, acting through GAL-R1 coupled to the adenylate cyclase/PKA-dependent signaling cascade via a Ptx-sensitive Gα protein.

show abstract

“…Methods applied were described earlier (21,(37)(38)(39)(40)(41). Applied primers for studies of NPB, NPW, NPBWR1 and similar to NPBWR2 gene expression (Table I) were purchased from the Laboratory of DNA Sequencing and Oligonucleotide Synthesis, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw.…”

Section: Methodsmentioning

confidence: 99%

Neuropeptide B (NPB) and neuropeptide W (NPW) system in cultured rat calvarial osteoblast-like (ROB) cells: NPW and NPB inhibit proliferative activity of ROB cells

Ziółkowska

Ruciński²,

Tyczewska³

et al. 2009

Int J Mol Med

View full text Add to dashboard Cite

Abstract. Neuropeptides B (NPB) and W (NPW) have been identified as endogenous ligands of two G-protein-coupled receptors, neuropeptides B/W receptor 1 (NPBWR1, formerly known as GPR7) and neuropeptides B/W receptor 2 (NPBWR2, formerly known as GPR8). In rodents where NPBWR2 is absent, its counterpart is named the similar to neuropeptides B/W receptor 2 (similar to NPBWR2, formerly GPR8-like). Both NPB and NPW play a role in the control of feeding, neuroendocrine axis functions, memory and learning processes as well as in pain regulation. The present study aimed to investigate the expression of NPB, NPW, NPBWR1 and the similar to NPBWR2 genes in cultured rat calvarial osteoblastlike (ROB) cells and the effects of both peptides on proliferative activity and osteocalcin secretion by ROB cells. Classic RT-PCR technique revealed the presence of ppNPB mRNA, ppNPW mRNA, and NPBWR1 mRNA, but not similar to NPBWR2 mRNA in ROB cells. QPCR revealed gradual (days 7, 14 and 21 of culture) increase of the ppNPB gene expression, while expression of ppNPW gene was the highest at day 14 and was comparable to that seen in freshly isolated cells. In ROB cells, expression of NPBWR1 gene was notable at day 7 of culture, lower at day 21, and negligible at day 14. Neither NPB nor NPW changed osteocalcin secretion by cultured osteoblast-like cells while both neuropeptides inhibited their proliferative activity. Results of the present study suggest that the systems of NPW, NPB and NPBWR1 directly regulate proliferative activity of cultured rat calvaria osteoblastlike cells. The physiological significance of this osteoblastic system remains unclear, and requires further investigation.

show abstract

Effects of neuropeptides B and W on the rat pituitary-adrenocortical axis: In vivo and in vitro studies

Cited by 12 publications

References 30 publications

NPBWR1 and NPBWR2: Implications in Energy Homeostasis, Pain, and Emotion

NPBWR1 and NPBWR2: Implications in Energy Homeostasis, Pain, and Emotion

Galanin stimulates cortisol secretion from human adrenocortical cells through the activation of galanin receptor subtype 1 coupled to the adenylate cyclase-dependent signaling cascade

Neuropeptide B (NPB) and neuropeptide W (NPW) system in cultured rat calvarial osteoblast-like (ROB) cells: NPW and NPB inhibit proliferative activity of ROB cells

Contact Info

Product

Resources

About