Effects of Nicotine on the Translation of Stem Cell Therapy

Chan, Alex; Huang, Ngan F

doi:10.2217/rme-2020-0032

Cited by 7 publications

(9 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, the selective α7 nAChR agonist ICH3, was shown to enhance MSCs migration [70]. Likewise, α-BTX, the α7 nAChR selective antagonist had the ability to block the detrimental effects of nicotine in several MSCs [67,71,72]. These data display an interesting potential for targeting ion channels that influence MSC function.…”

Section: Achrs As a Potential Target To Regulate Mscs Functionmentioning

confidence: 90%

“…The study also provides in vivo data demonstrating impaired migration of transplanted hMSCs to the bone marrow and spleen in mice as a result of nicotine exposure. Indeed, in a separate study, it has been suggested that higher doses of nicotine cause apoptosis and impair proliferation, while at non-toxic concentrations it decreases the migratory potential of MSCs [67].…”

Section: Expression Of Nachrs In Mscs Bone Marrow Mscs (Bm-mscs)mentioning

confidence: 99%

See 1 more Smart Citation

Acetylcholine Receptors in Mesenchymal Stem Cells

Alqahtani

Butcher

Ramage

et al. 2023

Stem Cells and Development

View full text Add to dashboard Cite

Mesenchymal stem cells (MSCs) are well known for their regenerative potential. Despite the fact that the ability of MSCs to proliferate and differentiate has been studied extensively, there still remains much to learn about the signalling mechanisms and pathways which control proliferation and influence differentiation phenotype. In recent years, there has been growing evidence for the utility of non-neuronal cholinergic signalling systems and that acetylcholine (ACh) plays an important, ubiquitous, role in cell-to-cell communication. Indeed, cholinergic signalling is hypothesised to occur in stem cells and ACh synthesis, as well as ACh receptor (AChR) expression, has been identified in several stem cell populations; including MSCs. Furthermore, AChRs have been found to influence MSC regenerative potential. In humans, there are two major classes of AChRs, muscarinic AChRs and nicotinic AChRs, with each class possessing several subtypes or subunits. In this review, the expression and function of AChRs in different types of MSC will be summarised with the aim of highlighting how AChRs play a pivotal role in regulating MSC regenerative function.

show abstract

Section: Achrs As a Potential Target To Regulate Mscs Functionmentioning

confidence: 90%

Section: Expression Of Nachrs In Mscs Bone Marrow Mscs (Bm-mscs)mentioning

confidence: 99%

Acetylcholine Receptors in Mesenchymal Stem Cells

Alqahtani

Butcher

Ramage

et al. 2023

Stem Cells and Development

View full text Add to dashboard Cite

show abstract

“…Smoker-derived MSCs generated significantly less bone matrix in comparison to cells isolated from nonsmokers ( Zhao et al, 2018 ). Upon autologous implantation of these scaffolds, the stability of implants was higher in nonsmokers ( Chan and Huang, 2020 ). Intriguingly, smoker-derived MSCs produced an elevated level of lipid contents even after several weeks of culture in a nicotine-free medium compared with cells isolated from nonsmokers ( Ng et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

“…The growing trend of cardiovascular patients, which has doubled from 1990 to 2019, necessitates urgent action for designing and implementing more efficient therapeutic strategies ( Roth et al, 2020 ). New policies resort to using the potential of stem cells, and thereby, nearly one-fifth of all stem cell-based clinical trials in the new decade of 2020 were designed to be conducted in the area of CVD ( Chan and Huang, 2020 ). Despite promising findings, the clinical translation of stem cell-based therapeutics has not reached a consensus yet.…”

Section: Introductionmentioning

confidence: 99%

The impact of high nicotine concentrations on the viability and cardiac differentiation of mesenchymal stromal cells: a barrier to regenerative therapy for smokers

Gheisari,

Nosrati,

Zare

et al. 2024

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Background: Current treatment methods are not successful in restoring the lost cardiomyocytes after injury. Stem cell-based strategies have attracted much attention in this regard. Smoking, as a strong cardiovascular risk factor, not only affects the cardiac cells adversely but also deteriorates the function of stem cells. Since mesenchymal stem cells (MSCs) are one of the popular candidates in cardiovascular disease (CVD) clinical trials, we investigated the impact of nicotine on the regenerative properties (viability and cardiac differentiation) of these cells.Methods: MSCs were isolated from rat bone marrow and characterized based on morphology, differentiation capability, and the expression of specific mesenchymal markers. The MTT assay was used to assess the viability of MSCs after being exposed to different concentrations of nicotine. Based on MTT findings and according to the concentration of nicotine in smokers’ blood, the growth curve and population doubling time were investigated for eight consecutive days. Cells were treated with 5-azacytidine (an inducer of cardiac differentiation), and then the expressions of cardiac-specific markers were calculated by qPCR.Results: MSCs were spindle-shaped, capable of differentiating into adipocyte and osteocyte, and expressed CD73 and CD90. The viability of MSCs was reduced upon exposure to nicotine in a concentration- and time-dependent manner. The growth curve showed that nicotine reduced the proliferation of MSCs, and treated cells needed more time to double. In addition, the expressions of GATA4 and troponin were downregulated in nicotine-treated cells on day 3. However, these two cardiac markers were overexpressed on day 7.Conclusion: Nicotine decreased normal growth and reduced the expression of cardiac markers in MSCs. This aspect is of eminent importance to smokers with cardiovascular disease who are candidates for stem cell therapy.

show abstract

“…For example, emerging research in preclinical models suggests that lifestyle choices such as tobacco exposure can negatively affect the efficacy of stem cell therapy for treatment of PAD. 110,111 Therefore, autologous stem cells from some patients may need preconditioning to reverse or reprogram the cells into a functional state before transplantation. In this regard, biomaterials can become a tool to modulate cellular function.…”

Section: Current Challenges and Future Directionsmentioning

confidence: 99%

Bioengineering Cell Therapy for Treatment of Peripheral Artery Disease

Huang,

Stern,

Oropeza

et al. 2024

ATVB

Self Cite

View full text Add to dashboard Cite

Peripheral artery disease is an atherosclerotic disease associated with limb ischemia that necessitates limb amputation in severe cases. Cell therapies comprised of adult mononuclear or stromal cells have been clinically tested and show moderate benefits. Bioengineering strategies can be applied to modify cell behavior and function in a controllable fashion. Using mechanically tunable or spatially controllable biomaterials, we highlight examples in which biomaterials can increase the survival and function of the transplanted cells to improve their revascularization efficacy in preclinical models. Biomaterials can be used in conjunction with soluble factors or genetic approaches to further modulate the behavior of transplanted cells and the locally implanted tissue environment in vivo. We critically assess the advances in bioengineering strategies such as 3-dimensional bioprinting and immunomodulatory biomaterials that can be applied to the treatment of peripheral artery disease and then discuss the current challenges and future directions in the implementation of bioengineering strategies.

show abstract

Effects of Nicotine on the Translation of Stem Cell Therapy

Cited by 7 publications

References 51 publications

Acetylcholine Receptors in Mesenchymal Stem Cells

Acetylcholine Receptors in Mesenchymal Stem Cells

The impact of high nicotine concentrations on the viability and cardiac differentiation of mesenchymal stromal cells: a barrier to regenerative therapy for smokers

Bioengineering Cell Therapy for Treatment of Peripheral Artery Disease

Contact Info

Product

Resources

About