Effects of Nitric Oxide on Ovulation and Ovarian Steroidogenesis and Prostaglandin Production in the Rabbit*

Yamauchi, Jun; Miyazaki, Toshihisa; Iwasaki, Shinya; Kishi, Ikuko; Kuroshima, Masako; Tei, Chisei; Yoshimura, Yasunori

doi:10.1210/endo.138.9.5392

Cited by 82 publications

(13 citation statements)

References 49 publications

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“…This indicates that there are species-specific differences in the pattern of eNOS protein expression. During folliculogenesis, NO not only regulates angiogenesis and blood vessel function, but also is involved in the regulation of steroidogenesis by influencing estradiol and progesterone production by granulosa and theca cells in several species (Olson et al 1996, Yamauchi et al 1997, Basini et al 1998, Rosselli et al 1998, Maul et al 2003. Therefore, it appears that NO plays a complex role in regulation of follicular development.…”

Section: Discussionmentioning

confidence: 99%

“…In mice, decreased or fully suppressed eNOS expression was associated with reduced ovulation rates (Yamauchi et al 1997, Jablonka-Shariff & Olson 1998. Moreover, it has been suggested that NO upregulates the ovulatory process by stimulation of prostaglandin production and/or through interactions with cytokines (Ahsan et al 1997, Yamauchi et al 1997Rosselli et al 1998, Gregg 2003, Mitchell et al 2004.…”

Section: Discussionmentioning

confidence: 99%

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Expression of endothelial nitric oxide synthase in the ovine ovary throughout the estrous cycle

Grazul-Bilska¹,

Navanukraw²,

Johnson³

et al. 2006

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This study was conducted to evaluate the expression of endothelial nitric oxide synthase (eNOS) in ovarian follicles and corpora lutea (CL) throughout the estrous cycle in sheep. Three experiments were conducted to (1) immunolocalize eNOS protein, (2) determine expression of mRNA for eNOS and its receptor guanylate cyclase 1 soluble b3 (GUCY1B3), and (3) co-localize eNOS and vascular endothelial growth factor (VEGF) proteins in the follicles and/or CL throughout the estrous cycle. In experiment 1, ovaries were collected from ewes treated with FSH, to induce follicular growth or atresia. In experiment 2, ovaries were collected from ewes treated with FSH and hCG to induce follicular growth and ovulation. In experiment 3, ovaries were collected from superovulated ewes to generate multiple CL on days 2, 4, 10, and 15 of the estrous cycle. In experiments 1 and 2, the expression of eNOS protein was detected in the blood vessels of the theca externa and interna of healthy ovarian follicles. However, in early and advanced atretic follicles, eNOS protein expression was absent or reduced. During the immediate postovulatory period, eNOS protein expression was detected in thecal-derived cells that appeared to be invading the granulosa layer. Expression of eNOS mRNA tended to increase in granulosa cells at 12 and 24 h, and in theca cells 48 h after hCG injection. In experiment 3, eNOS protein was located in the blood vessels of the CL during the estrous cycle. Dual localization of eNOS and VEGF proteins in the CL demonstrated that both were found in the blood vessels.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Expression of endothelial nitric oxide synthase in the ovine ovary throughout the estrous cycle

Grazul-Bilska¹,

Navanukraw²,

Johnson³

et al. 2006

Reproduction

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“…In the ovary and luteal tissue, endogenous NO, besides regulating the activity of key enzymes such as P450/scc, ADP-ribosylating enzymes, dehydrogenases, phosphatases and other transcription factors (Gow & Ischiropoulos 2001), may also inhibit 17b-oestradiol synthesis (Yamauchi et al 1997), which exerts a well-known luteotrophic action in rabbits (Holt 1989), or stimulate luteal PGF2a release through the activation of cyclooxygenase (Estevez et al 2002). Within the CL, overproduction of NO during luteolysis may target nitration and oxidation in specific luteal cellular compartments thereby affecting specific protein function as well as causing lipid peroxidation (Aten et al 1998, Motta et al 2001 or DNA damage (Beckman et al 1990).…”

Section: Discussionmentioning

confidence: 99%

Expression patterns of cytokines, p53 and nitric oxide synthase isoenzymes in corpora lutea of pseudopregnant rabbits during spontaneous luteolysis

Boiti¹,

Guelfi²,

Zerani³

et al. 2004

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The gene expressions for macrophage chemoattractant protein-1 (MCP-1), interleukin (IL)-1b, IL-2 and p53 were examined by semi-quantitative RT-PCR in corpora lutea (CL) of rabbits during spontaneous luteolysis at days 13, 15, 18 and 22 of pseudopregnancy. In the same luteal tissue, total activity of nitric oxide (NO) synthase (NOS) and genes for both endothelial (eNOS) and inducible (iNOS) isoforms were also analysed. From day 13 to 15, MCP-1 and IL-1b mRNA levels rose (P # 0.01) almost 2-fold, and the transcript for p53 almost 8-fold, but then all dropped (P # 0.05) from day 18 onward. IL-2 mRNA abundance was higher (P # 0.01) on day 13 and then gradually declined. During luteolysis, eNOS mRNA decreased 40% (P # 0.05) by day 15, but thereafter remained unchanged, while iNOS mRNA was barely detectable and did not show any clear age-related pattern throughout the late luteal stages. Total NOS activity progressively increased (P # 0.01) from day 13 to 18 of pseudopregnancy and then dropped to the lowest (P # 0.01) levels on day 22. Luteal progesterone content also declined during CL regression from 411 to 17 pg/mg found on days 13 and 22 respectively, in parallel with the decrease in blood progesterone concentrations. These data further support a physiological role of NO as modulator of luteal demise in rabbits. Locally, luteal cytokines may be involved in the up-regulation of NOS activity, while downstream NO may inhibit steroroidogenesis and induce expression of p53 gene after removal of the protective action of progesterone.

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“…It is not clear which NOS isoform(s) is functional in porcine oocyte meiotic maturation. Aminoguanidine (AG) is a specific and selective iNOS inhibitor while Nω-nitro-L-arginine (L-NNA) and Nω-nitro-L-arginine methyl ester (L-NAME) inhibit both eNOS and iNOS (Sengoku et al, 2001;Nakamura et al, 2002;Yamauchi et al, 1997). The present study investigated which NOS isoform(s) is involved in porcine oocyte meiotic maturation and the functions of cumulus cells, and whether surrounding cumulus cells function in the process.…”

Section: Introductionmentioning

confidence: 99%

Effects of nitric oxide synthase inhibitors on porcine oocyte meiotic maturation

Tao

Xie

Hong

et al. 2005

Zygote

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As an important biological messenger, nitric oxide (NO) exhibits a wide range of effects during physiological and pathophysiological processes, including mammalian oocyte meiotic maturation. The present study investigated whether NO derived from two nitric oxide synthase (NOS) isoforms, inducible NOS (iNOS) or endothelial NOS (eNOS), is involved in the meiotic maturation of porcine oocytes. Meanwhile, the cumulus cells' function in meiotic maturation and their interaction with oocyte development and degeneration were also investigated using cumulus-enclosed oocytes (CEOs) and denuded oocytes (DOs). Different inhibitors for NOS were supplemented to the medium. Cumulus expansion, cumulus cell DNA fragmentation and oocyte meiotic resumption were evaluated 48 h after incubation. Aminoguanidine (AG), a selective inhibitor for iNOS, suppressed cumulus expansion and inhibited CEOs to resume meiosis (p < 0.05), but did not inhibit cumulus cell DNA fragmentation. Both Nω-nitro-L-arginine (L-NNA) and Nω-nitro-L-arginine methyl ester (L-NAME), inhibitors for both iNOS and eNOS, delayed cumulus expansion, inhibited cumulus cell DNA fragmentation and inhibited CEOs to resume meiosis. Such effects were not seen in DOs. These results indicate that iNOS-derived NO is necessary for cumulus expansion and meiotic maturation by mediating the function of the surrounding cumulus cells, and eNOS-derived NO is also involved in porcine meiotic maturation.

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Effects of Nitric Oxide on Ovulation and Ovarian Steroidogenesis and Prostaglandin Production in the Rabbit*

Cited by 82 publications

References 49 publications

Expression of endothelial nitric oxide synthase in the ovine ovary throughout the estrous cycle

Expression of endothelial nitric oxide synthase in the ovine ovary throughout the estrous cycle

Expression patterns of cytokines, p53 and nitric oxide synthase isoenzymes in corpora lutea of pseudopregnant rabbits during spontaneous luteolysis

Effects of nitric oxide synthase inhibitors on porcine oocyte meiotic maturation

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